##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AccII Aor51HI AscI
Asp718I AviII AvrII BglI BglII
BlnI BlpI Bpu1102I BsaI BsiWI
BsmBI BspDI BsrBI BssHII Bst1107I
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI Eco47III EcoO65I FspI
HindIII HpaI KpnI KspI MfeI
MluI MunI MvnI NheI NotI
NruI PacI PmeI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SmaI SnaBI Sse8387I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AcyI 4361 GGCGCC G[GA]^CG[CT]C
AgeI 2597 ACCGGT A^CCGGT
AhdI 908 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1235 GTGCAC G^TGCAC
ApaI 24 GGGCCC GGGCC^C
ApaLI 1235 GTGCAC G^TGCAC
AspEI 908 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4228 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4424 TGGCCA TGG^CCA
BamHI 795 GGATCC G^GATCC
BanI 4361 GGCGCC G^G[CT][GA]CC
BbeI 4361 GGCGCC GGCGC^C
BbrPI 2308 CACGTG CAC^GTG
BbvCI 4332 CCTCAGC CCTCAGC(-5/-2)
BcgI 1614 CGAACACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 2308 CACGTG [CT]AC^GT[GA]
BsaHI 4361 GGCGCC G[GA]^CG[CT]C
BsiEI 162 CGGCCG CG[GA][CT]^CG
BspHI 682 TCATGA T^CATGA
BstBI 2196 TTCGAA TT^CGAA
BstXI 4475 CCATAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 2489 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 6786 GACCCAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 162 CGGCCG C^GGCCG
Eam1105I 908 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 162 CGGCCG C^GGCCG
Eco52I 162 CGGCCG C^GGCCG
EcoNI 5330 CCTTTAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 449 GATATC GAT^ATC
FseI 133 GGCCGGCC GGCCGG^CC
HaeII 4361 GGCGCC [GA]GCGC^[CT]
HgaI 3242 GCGTC GACGC(5/10)
Hin1I 4361 GGCGCC G[GA]^CG[CT]C
KasI 4361 GGCGCC G^GCGCC
MluNI 4424 TGGCCA TGG^CCA
MscI 4424 TGGCCA TGG^CCA
NarI 4361 GGCGCC GG^CGCC
NdeI 2479 CATATG CA^TATG
NspV 2196 TTCGAA TT^CGAA
PflFI 4228 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1878 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2597 ACCGGT A^CCGGT
PmaCI 2308 CACGTG CAC^GTG
PmlI 2308 CACGTG CAC^GTG
PshAI 2092 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1853 CAGCTG CAG^CTG
RcaI 682 TCATGA T^CATGA
SapI 3710 GCTCTTC GCTCTTC(1/4)
SfoI 4361 GGCGCC GGC^GCC
SfuI 2196 TTCGAA TT^CGAA
SgrAI 2596 CACCGGTG C[GA]^CCGG[CT]G
SphI 6469 GCATGC GCATG^C
Tth111I 4228 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1878 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1092 CCAGGGAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1016 GTAGAC GT^[AC][GT]AC
2345 GTAGAC
AccIII 180 TCCGGA T^CCGGA
5288 TCCGGA
AclI 2703 AACGTT AA^CGTT
6361 AACGTT
AflII 3029 CTTAAG C^TTAAG
4223 CTTAAG
AlwNI 267 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3269 CAGTGGCTG
AocI 953 CCTGAGG CC^T[ACGT]AGG
2546 CCTCAGG
AvaI 4341 CTCGAG C^[CT]CG[GA]G
6183 CTCGAG
BciVI 2115 GGATAC GTATCC(6/5)
3898 GTATCC
BfrI 3029 CTTAAG C^TTAAG
4223 CTTAAG
BsaBI 290 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1522 GATGGACATC
BseAI 180 TCCGGA T^CCGGA
5288 TCCGGA
BseRI 75 CTCCTC GAGGAG(10/8)
1199 GAGGAG
BsgI 7053 CTGCAC GTGCAG(16/14)
2128 GTGCAG
BsoBI 4341 CTCGAG C^[CT]CG[GA]G
6183 CTCGAG
BspEI 180 TCCGGA T^CCGGA
5288 TCCGGA
BspMI 4460 ACCTGC ACCTGC(4/8)
4888 GCAGGT
Bsu36I 953 CCTGAGG CC^T[ACGT]AGG
2546 CCTCAGG
Eco81I 953 CCTGAGG CC^T[ACGT]AGG
2546 CCTCAGG
EcoRI 2865 GAATTC G^AATTC
6003 GAATTC
MamI 290 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1522 GATGGACATC
MroI 180 TCCGGA T^CCGGA
5288 TCCGGA
NaeI 134 GCCGGC GCC^GGC
160 GCCGGC
NgoMIV 134 GCCGGC G^CCGGC
160 GCCGGC
PaeR7I 4341 CTCGAG C^TCGAG
6183 CTCGAG
Psp1406I 2703 AACGTT AA^CGTT
6361 AACGTT
SpeI 3759 ACTAGT A^CTAGT
6215 ACTAGT
StuI 2287 AGGCCT AGG^CCT
4150 AGGCCT
SwaI 1146 ATTTAAAT ATTT^AAAT
2978 ATTTAAAT
XhoI 4341 CTCGAG C^TCGAG
6183 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BanII BbsI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsaWI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspLU11I BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI PshBI
PstI RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI