##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AviII BalI BamHI BbrPI
BbvCI BcgI BglI BpmI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BstBI ClaI CpoI DraIII DrdI
Eam1105I Eco47III EcoRI FseI FspI
HpaI KpnI MluI MluNI MroI
MscI NcoI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SapI
SbfI SexAI SfiI SfuI SpeI
Sse8387I
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Alw44I 2342 GTGCAC G^TGCAC
ApaLI 2342 GTGCAC G^TGCAC
AspI 2036 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5129 CCTAGG C^CTAGG
BbeI 193 GGCGCC GGCGC^C
BciVI 2362 GGATAC GTATCC(6/5)
BlnI 5129 CCTAGG C^CTAGG
BsaAI 2933 TACGTA [CT]AC^GT[GA]
BsaI 1183 GGTCTC GGTCTC(1/5)
BsrDI 2618 GCAATG GCAATG(2/0)
BssSI 458 CTCGTG CACGAG(-5/-1)
Bst1107I 4958 GTATAC GTA^TAC
BstXI 4615 CCACATAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4958 GTATAC GTA^TAC
EagI 147 CGGCCG C^GGCCG
EclXI 147 CGGCCG C^GGCCG
Eco52I 147 CGGCCG C^GGCCG
EcoNI 4664 CCTTCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 4208 ATGCAT ATGCA^T
HindIII 1447 AAGCTT A^AGCTT
KasI 193 GGCGCC G^GCGCC
NaeI 149 GCCGGC GCC^GGC
NarI 193 GGCGCC GG^CGCC
NgoMIV 149 GCCGGC G^CCGGC
NheI 130 GCTAGC G^CTAGC
NsiI 4208 ATGCAT ATGCA^T
PaeR7I 2482 CTCGAG C^TCGAG
PflFI 2036 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1941 CAGCTG CAG^CTG
SalI 2001 GTCGAC G^TCGAC
SfoI 193 GGCGCC GGC^GCC
SgrAI 542 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 125 CCCGGG CCC^GGG
SnaBI 2933 TACGTA TAC^GTA
StuI 4702 AGGCCT AGG^CCT
SwaI 3515 ATTTAAAT ATTT^AAAT
Tth111I 2036 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2482 CTCGAG C^TCGAG
XmaCI 125 CCCGGG C^CCGGG
XmaI 125 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 2001 GTCGAC GT^[AC][GT]AC
4958 GTATAC
AcyI 193 GGCGCC G[GA]^CG[CT]C
237 GGCGTC
AflII 4405 CTTAAG C^TTAAG
5961 CTTAAG
AlwNI 1941 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3610 CAGTTACTG
AocI 6002 CCTAAGG CC^T[ACGT]AGG
6675 CCTAAGG
BanII 465 GAGCTC G[GA]GC[CT]^C
2165 GAGCTC
BfrI 4405 CTTAAG C^TTAAG
5961 CTTAAG
BlpI 95 GCTCAGC GC^T[ACGT]AGC
2162 GCTGAGC
Bpu1102I 95 GCTCAGC GC^T[ACGT]AGC
2162 GCTGAGC
BsaBI 2072 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4711 GATGATGATC
BsaHI 193 GGCGCC G[GA]^CG[CT]C
237 GGCGTC
BseRI 2475 GAGGAG GAGGAG(10/8)
3374 GAGGAG
BsiEI 82 CGGTCG CG[GA][CT]^CG
147 CGGCCG
BsmI 3102 GCATTC GAATGC(1/-1)
6192 GCATTC
BsrFI 149 GCCGGC [GA]^CCGG[CT]
543 GCCGGT
BstAPI 1316 GCAATTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6374 GCAATATATGC
BstEII 3079 GGTTACC G^GT[ACGT]ACC
6751 GGTCACC
BstPI 3079 GGTTACC G^GT[ACGT]ACC
6751 GGTCACC
Bsu36I 6002 CCTAAGG CC^T[ACGT]AGG
6675 CCTAAGG
CelII 95 GCTCAGC GC^T[ACGT]AGC
2162 GCTGAGC
Cfr10I 149 GCCGGC [GA]^CCGG[CT]
543 GCCGGT
DraII 513 AGGTCCT [GA]G^G[ACGT]CC[CT]
1790 AGGTCCC
EaeI 147 CGGCCG [CT]^GGCC[GA]
3224 CGGCCA
Eco81I 6002 CCTAAGG CC^T[ACGT]AGG
6675 CCTAAGG
EcoO109I 513 AGGTCCT [GA]G^G[ACGT]CC[CT]
1790 AGGTCCC
EcoO65I 3079 GGTTACC G^GT[ACGT]ACC
6751 GGTCACC
HaeII 193 GGCGCC [GA]GCGC^[CT]
3112 AGCGCC
HgaI 238 GCGTC GACGC(5/10)
437 GCGTC
Hin1I 193 GGCGCC G[GA]^CG[CT]C
237 GGCGTC
MamI 2072 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4711 GATGATGATC
PflMI 1168 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1445 CCAAGCTTTGG
PpuMI 513 AGGTCCT [GA]G^G[AT]CC[CT]
1790 AGGTCCC
SacI 465 GAGCTC GAGCT^C
2165 GAGCTC
ScaI 378 AGTACT AGT^ACT
1696 AGTACT
SphI 1437 GCATGC GCATG^C
6370 GCATGC
Van91I 1168 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1445 CCAAGCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AvaI AvaII BanI
BbsI BbvI BclI BcnI BfaI
BglII BmyI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspHI BspLU11I
BspMI BsrBI BsrGI BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EarI EcoRII EcoRV
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PleI
PshBI PstI RcaI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XcmI XhoII
XmnI