##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AgeI Aor51HI AscI
BamHI BbrPI BcgI BglI BsaAI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstPI Cfr10I ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FseI KspI MfeI MluI MroI
MunI NaeI NgoMIV NotI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacII SbfI SexAI SfiI SfuI
SgrAI SnaBI Sse8387I StuI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 152 GGTACC G^GTACC
AccII 4699 CGCG CG^CG
AclI 1640 AACGTT AA^CGTT
AhdI 5596 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 5476 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2831 CCTTAGG CC^T[ACGT]AGG
Asp718I 152 GGTACC G^GTACC
AspEI 5596 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1278 CCCGGG C^[CT]CG[GA]G
AviII 5827 TGCGCA TGC^GCA
BbeI 1380 GGCGCC GGCGC^C
BciVI 637 GTATCC GTATCC(6/5)
BclI 5516 TGATCA T^GATCA
BsoBI 1278 CCCGGG C^[CT]CG[GA]G
Bsp1407I 6271 TGTACA T^GTACA
BsrGI 6271 TGTACA T^GTACA
BstAPI 2204 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 4699 CGCG CG^CG
BstXI 1571 CCAGCTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2831 CCTTAGG CC^T[ACGT]AGG
Eam1105I 5596 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2831 CCTTAGG CC^T[ACGT]AGG
EcoNI 50 CCTAGTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 5516 TGATCA T^GATCA
FspI 5827 TGCGCA TGC^GCA
HaeII 1380 GGCGCC [GA]GCGC^[CT]
HgaI 5803 GCGTC GACGC(5/10)
HpaI 3868 GTTAAC GTT^AAC
KasI 1380 GGCGCC G^GCGCC
KpnI 152 GGTACC GGTAC^C
MvnI 4699 CGCG CG^CG
NarI 1380 GGCGCC GG^CGCC
NdeI 2891 CATATG CA^TATG
NheI 6127 GCTAGC G^CTAGC
NruI 4698 TCGCGA TCG^CGA
PflMI 747 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 5450 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 1640 AACGTT AA^CGTT
SacI 5571 GAGCTC GAGCT^C
SalI 5640 GTCGAC G^TCGAC
SapI 5568 GAAGAGC GCTCTTC(1/4)
ScaI 2554 AGTACT AGT^ACT
SfoI 1380 GGCGCC GGC^GCC
SmaI 1278 CCCGGG CCC^GGG
SspBI 6271 TGTACA T^GTACA
Van91I 747 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2926 CCAACCCTACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1278 CCCGGG C^CCGGG
XmaI 1278 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 1380 GGCGCC G[GA]^CG[CT]C
5802 GGCGTC
AflII 10 CTTAAG C^TTAAG
1688 CTTAAG
AflIII 3992 ACATGT A^C[GA][CT]GT
4487 ACATGT
Alw44I 1140 GTGCAC G^TGCAC
2061 GTGCAC
ApaI 3845 GGGCCC GGGCC^C
5410 GGGCCC
ApaLI 1140 GTGCAC G^TGCAC
2061 GTGCAC
AspI 2142 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5811 GACTTAGTC
AvaII 3486 GGACC G^G[AT]CC
5451 GGTCC
BanI 152 GGTACC G^G[CT][GA]CC
1380 GGCGCC
BfrI 10 CTTAAG C^TTAAG
1688 CTTAAG
BlpI 3902 GCTGAGC GC^T[ACGT]AGC
4901 GCTAAGC
Bpu1102I 3902 GCTGAGC GC^T[ACGT]AGC
4901 GCTAAGC
BsaBI 3344 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5684 GATTTTGATC
BsaHI 1380 GGCGCC G[GA]^CG[CT]C
5802 GGCGTC
BspLU11I 3992 ACATGT A^CATGT
4487 ACATGT
CelII 3902 GCTGAGC GC^T[ACGT]AGC
4901 GCTAAGC
FauI 4714 CCCGC CCCGC(4/6)
3862 GCGGG
Hin1I 1380 GGCGCC G[GA]^CG[CT]C
5802 GGCGTC
HindIII 4363 AAGCTT A^AGCTT
6825 AAGCTT
MamI 3344 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5684 GATTTTGATC
MslI 2995 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5675 CATGAGTGTG
PflFI 2142 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5811 GACTTAGTC
PstI 623 CTGCAG CTGCA^G
5089 CTGCAG
SpeI 698 ACTAGT A^CTAGT
4021 ACTAGT
Tth111I 2142 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5811 GACTTAGTC
XbaI 2342 TCTAGA T^CTAGA
3853 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvrII BalI BanII BbsI
BbvCI BbvI BcnI BfaI BglII
BlnI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI Bst1107I BstF5I BstNI BstYI
BstZ17I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PshBI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI