##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AatII AflII AhdI AscI AspEI
AvrII BbrPI BclI BfrI BlnI
BsaAI BsiWI BspLU11I Bst1107I BstZ17I
DraI DrdI Eam1105I EcoT22I FbaI
HincII HindII HpaI MfeI MluI
MunI NotI NruI NsiI PmaCI
PmeI PmlI PshAI SalI SbfI
SexAI SfiI SgrAI SnaBI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 3527 GGTACC G^GTACC
AclI 847 AACGTT AA^CGTT
AflIII 3917 ACGTGT A^C[GA][CT]GT
Alw44I 2950 GTGCAC G^TGCAC
ApaLI 2950 GTGCAC G^TGCAC
AseI 1444 ATTAAT AT^TAAT
AsnI 1444 ATTAAT AT^TAAT
Asp718I 3527 GGTACC G^GTACC
AspI 7362 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5994 TGCGCA TGC^GCA
BamHI 1221 GGATCC G^GATCC
BcgI 5391 CGAGCGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1998 GGATAC GTATCC(6/5)
BsaBI 2865 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 4588 GAATGC GAATGC(1/-1)
Bsp1407I 1710 TGTACA T^GTACA
BspHI 1002 TCATGA T^CATGA
BsrGI 1710 TGTACA T^GTACA
BssHII 4281 GCGCGC G^CGCGC
BssSI 2807 CACGAG CACGAG(-5/-1)
CpoI 3532 CGGTCCG CG^G[AT]CCG
EagI 6524 CGGCCG C^GGCCG
EclXI 6524 CGGCCG C^GGCCG
Eco52I 6524 CGGCCG C^GGCCG
EcoRV 1501 GATATC GAT^ATC
FseI 6606 GGCCGGCC GGCCGG^CC
FspI 5994 TGCGCA TGC^GCA
KpnI 3527 GGTACC GGTAC^C
KspI 6662 CCGCGG CCGC^GG
MamI 2865 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 3249 GCTAGC G^CTAGC
PacI 15 TTAATTAA TTAAT^TAA
PflFI 7362 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1444 ATTAAT AT^TAAT
Psp1406I 847 AACGTT AA^CGTT
PvuI 4841 CGATCG CGAT^CG
RcaI 1002 TCATGA T^CATGA
RsrII 3532 CGGTCCG CG^G[AT]CCG
SacI 7418 GAGCTC GAGCT^C
SacII 6662 CCGCGG CCGC^GG
ScaI 4195 AGTACT AGT^ACT
SpeI 1044 ACTAGT A^CTAGT
SspBI 1710 TGTACA T^GTACA
Tth111I 7362 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6285 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 766 GTCTAC GT^[AC][GT]AC
1393 GTCTAC
Aor51HI 5393 AGCGCT AGC^GCT
5694 AGCGCT
BbsI 2317 GAAGAC GAAGAC(2/6)
2422 GAAGAC
BglI 1381 GCCTATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4331 GCCAGGGTGGC
BglII 3015 AGATCT A^GATCT
3232 AGATCT
BlpI 3936 GCTCAGC GC^T[ACGT]AGC
6955 GCTCAGC
Bpu1102I 3936 GCTCAGC GC^T[ACGT]AGC
6955 GCTCAGC
BpuAI 2317 GAAGAC GAAGAC(2/6)
2422 GAAGAC
BspDI 2162 ATCGAT AT^CGAT
4498 ATCGAT
BspMI 5051 ACCTGC ACCTGC(4/8)
5257 ACCTGC
CelII 3936 GCTCAGC GC^T[ACGT]AGC
6955 GCTCAGC
ClaI 2162 ATCGAT AT^CGAT
4498 ATCGAT
Eco47III 5393 AGCGCT AGC^GCT
5694 AGCGCT
EcoNI 1818 CCTTTACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4245 CCTGCTGAAGG
PvuII 4574 CAGCTG CAG^CTG
5630 CAGCTG
SapI 4788 GAAGAGC GCTCTTC(1/4)
4258 GCTCTTC
SmaI 4111 CCCGGG CCC^GGG
6039 CCCGGG
SphI 2989 GCATGC GCATG^C
6911 GCATGC
SspI 1688 AATATT AAT^ATT
2489 AATATT
XmaCI 4111 CCCGGG C^CCGGG
6039 CCCGGG
XmaI 4111 CCCGGG C^CCGGG
6039 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AgeI AluI AlwI AlwNI
AocI ApaI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvCI BbvI BcnI BfaI
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspEI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstBI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoO109I EcoO65I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NlaIII NlaIV NspI
NspV PaeR7I PflMI PinAI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SfuI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII
XmnI