##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AccIII AscI AvrII BamHI
BcgI BglI BlnI BseAI BsiWI
BspDI BspEI BssHII BssSI BstBI
ClaI CpoI DraIII DrdI EcoRV
FseI HpaI KspI MfeI MluI
MroI MunI NcoI NdeI NheI
NruI NspV PacI PaeR7I PmeI
PshAI PvuI RsrII SacI SacII
SalI SapI SbfI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AclI 2083 AACGTT AA^CGTT
ApaI 3550 GGGCCC GGGCC^C
AspI 4258 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5354 CTCGGG C^[CT]CG[GA]G
BanII 3550 GGGCCC G[GA]GC[CT]^C
BbrPI 3618 CACGTG CAC^GTG
BsaAI 3618 CACGTG [CT]AC^GT[GA]
BsiEI 3 CGGCCG CG[GA][CT]^CG
BsmBI 4742 GAGACG CGTCTC(1/5)
BsoBI 5354 CTCGGG C^[CT]CG[GA]G
Bsp1407I 151 TGTACA T^GTACA
BsrBI 3497 GAGCGG CCGCTC(-3/-3)
BsrGI 151 TGTACA T^GTACA
BstEII 5966 GGTGACC G^GT[ACGT]ACC
BstPI 5966 GGTGACC G^GT[ACGT]ACC
BstXI 5411 CCAGGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 3 CGGCCG C^GGCCG
EclXI 3 CGGCCG C^GGCCG
Eco52I 3 CGGCCG C^GGCCG
EcoO65I 5966 GGTGACC G^GT[ACGT]ACC
EcoRI 6164 GAATTC G^AATTC
NaeI 29 GCCGGC GCC^GGC
NgoMIV 29 GCCGGC G^CCGGC
NotI 2 GCGGCCGC GC^GGCCGC
PflFI 4258 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3618 CACGTG CAC^GTG
PmlI 3618 CACGTG CAC^GTG
PpuMI 5695 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 2083 AACGTT AA^CGTT
PvuII 3561 CAGCTG CAG^CTG
SexAI 1313 ACCAGGT A^CC[AT]GGT
SspBI 151 TGTACA T^GTACA
StuI 4349 AGGCCT AGG^CCT
Tth111I 4258 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1247 CCAGTATATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 3766 GGTACC G^GTACC
4332 GGTACC
AgeI 570 ACCGGT A^CCGGT
3734 ACCGGT
AhdI 1367 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2867 GACCACATGTC
Alw44I 626 GTGCAC G^TGCAC
3817 GTGCAC
Aor51HI 676 AGCGCT AGC^GCT
1669 AGCGCT
ApaLI 626 GTGCAC G^TGCAC
3817 GTGCAC
Asp718I 3766 GGTACC G^GTACC
4332 GGTACC
AspEI 1367 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2867 GACCACATGTC
AviII 195 TGCGCA TGC^GCA
4243 TGCGCA
BbeI 321 GGCGCC GGCGC^C
351 GGCGCC
BciVI 5795 GGATAC GTATCC(6/5)
4661 GTATCC
BclI 4170 TGATCA T^GATCA
5589 TGATCA
BlpI 4795 GCTCAGC GC^T[ACGT]AGC
4880 GCTCAGC
Bpu1102I 4795 GCTCAGC GC^T[ACGT]AGC
4880 GCTCAGC
BsaWI 570 ACCGGT [AT]^CCGG[AT]
3734 ACCGGT
BsrDI 1940 CATTGC GCAATG(2/0)
3096 CATTGC
BstAPI 254 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5365 GCAACCTCTGC
CelII 4795 GCTCAGC GC^T[ACGT]AGC
4880 GCTCAGC
Eam1105I 1367 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2867 GACCACATGTC
Eco47III 676 AGCGCT AGC^GCT
1669 AGCGCT
FauI 3608 CCCGC CCCGC(4/6)
5827 CCCGC
FbaI 4170 TGATCA T^GATCA
5589 TGATCA
FspI 195 TGCGCA TGC^GCA
4243 TGCGCA
HincII 952 GTTGAC GT[CT]^[GA]AC
6130 GTCAAC
HindII 952 GTTGAC GT[CT]^[GA]AC
6130 GTCAAC
HindIII 2227 AAGCTT A^AGCTT
2528 AAGCTT
KasI 321 GGCGCC G^GCGCC
351 GGCGCC
KpnI 3766 GGTACC GGTAC^C
4332 GGTACC
NarI 321 GGCGCC GG^CGCC
351 GGCGCC
PinAI 570 ACCGGT A^CCGGT
3734 ACCGGT
ScaI 4717 AGTACT AGT^ACT
5274 AGTACT
SfaNI 6361 GATGC GCATC(5/9)
2939 GCATC
SfoI 321 GGCGCC GGC^GCC
351 GGCGCC
SpeI 2162 ACTAGT A^CTAGT
2300 ACTAGT
SwaI 2414 ATTTAAAT ATTT^AAAT
2756 ATTTAAAT
TaqI 124 TCGA T^CGA
3743 TCGA
TthHB8I 124 TCGA T^CGA
3743 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI AlwI
AlwNI AocI ApoI AseI AsnI
Asp700I AspHI AvaII BalI BanI
BbsI BbvCI BbvI BcnI BfaI
BfrI BglII BmyI BpmI BpuAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BspLU11I
BspMI BsrFI BsrI BssKI Bst1107I
BstF5I BstNI BstUI BstYI BstZ17I
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PshBI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XbaI XhoII XmnI