################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AatII AccIII AscI AvrII BamHI BcgI BglI BlnI BseAI BsiWI BspDI BspEI BssHII BssSI BstBI ClaI CpoI DraIII DrdI EcoRV FseI HpaI KspI MfeI MluI MroI MunI NcoI NdeI NheI NruI NspV PacI PaeR7I PmeI PshAI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfuI SgrAI SmaI SnaBI SphI Sse8387I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AclI 2083 AACGTT AA^CGTT ApaI 3550 GGGCCC GGGCC^C AspI 4258 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 5354 CTCGGG C^[CT]CG[GA]G BanII 3550 GGGCCC G[GA]GC[CT]^C BbrPI 3618 CACGTG CAC^GTG BsaAI 3618 CACGTG [CT]AC^GT[GA] BsiEI 3 CGGCCG CG[GA][CT]^CG BsmBI 4742 GAGACG CGTCTC(1/5) BsoBI 5354 CTCGGG C^[CT]CG[GA]G Bsp1407I 151 TGTACA T^GTACA BsrBI 3497 GAGCGG CCGCTC(-3/-3) BsrGI 151 TGTACA T^GTACA BstEII 5966 GGTGACC G^GT[ACGT]ACC BstPI 5966 GGTGACC G^GT[ACGT]ACC BstXI 5411 CCAGGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EagI 3 CGGCCG C^GGCCG EclXI 3 CGGCCG C^GGCCG Eco52I 3 CGGCCG C^GGCCG EcoO65I 5966 GGTGACC G^GT[ACGT]ACC EcoRI 6164 GAATTC G^AATTC NaeI 29 GCCGGC GCC^GGC NgoMIV 29 GCCGGC G^CCGGC NotI 2 GCGGCCGC GC^GGCCGC PflFI 4258 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3618 CACGTG CAC^GTG PmlI 3618 CACGTG CAC^GTG PpuMI 5695 GGGACCT [GA]G^G[AT]CC[CT] Psp1406I 2083 AACGTT AA^CGTT PvuII 3561 CAGCTG CAG^CTG SexAI 1313 ACCAGGT A^CC[AT]GGT SspBI 151 TGTACA T^GTACA StuI 4349 AGGCCT AGG^CCT Tth111I 4258 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1247 CCAGTATATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 3766 GGTACC G^GTACC 4332 GGTACC AgeI 570 ACCGGT A^CCGGT 3734 ACCGGT AhdI 1367 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2867 GACCACATGTC Alw44I 626 GTGCAC G^TGCAC 3817 GTGCAC Aor51HI 676 AGCGCT AGC^GCT 1669 AGCGCT ApaLI 626 GTGCAC G^TGCAC 3817 GTGCAC Asp718I 3766 GGTACC G^GTACC 4332 GGTACC AspEI 1367 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2867 GACCACATGTC AviII 195 TGCGCA TGC^GCA 4243 TGCGCA BbeI 321 GGCGCC GGCGC^C 351 GGCGCC BciVI 5795 GGATAC GTATCC(6/5) 4661 GTATCC BclI 4170 TGATCA T^GATCA 5589 TGATCA BlpI 4795 GCTCAGC GC^T[ACGT]AGC 4880 GCTCAGC Bpu1102I 4795 GCTCAGC GC^T[ACGT]AGC 4880 GCTCAGC BsaWI 570 ACCGGT [AT]^CCGG[AT] 3734 ACCGGT BsrDI 1940 CATTGC GCAATG(2/0) 3096 CATTGC BstAPI 254 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5365 GCAACCTCTGC CelII 4795 GCTCAGC GC^T[ACGT]AGC 4880 GCTCAGC Eam1105I 1367 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2867 GACCACATGTC Eco47III 676 AGCGCT AGC^GCT 1669 AGCGCT FauI 3608 CCCGC CCCGC(4/6) 5827 CCCGC FbaI 4170 TGATCA T^GATCA 5589 TGATCA FspI 195 TGCGCA TGC^GCA 4243 TGCGCA HincII 952 GTTGAC GT[CT]^[GA]AC 6130 GTCAAC HindII 952 GTTGAC GT[CT]^[GA]AC 6130 GTCAAC HindIII 2227 AAGCTT A^AGCTT 2528 AAGCTT KasI 321 GGCGCC G^GCGCC 351 GGCGCC KpnI 3766 GGTACC GGTAC^C 4332 GGTACC NarI 321 GGCGCC GG^CGCC 351 GGCGCC PinAI 570 ACCGGT A^CCGGT 3734 ACCGGT ScaI 4717 AGTACT AGT^ACT 5274 AGTACT SfaNI 6361 GATGC GCATC(5/9) 2939 GCATC SfoI 321 GGCGCC GGC^GCC 351 GGCGCC SpeI 2162 ACTAGT A^CTAGT 2300 ACTAGT SwaI 2414 ATTTAAAT ATTT^AAAT 2756 ATTTAAAT TaqI 124 TCGA T^CGA 3743 TCGA TthHB8I 124 TCGA T^CGA 3743 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflII AflIII AluI AlwI AlwNI AocI ApoI AseI AsnI Asp700I AspHI AvaII BalI BanI BbsI BbvCI BbvI BcnI BfaI BfrI BglII BmyI BpmI BpuAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspLU11I BspMI BsrFI BsrI BssKI Bst1107I BstF5I BstNI BstUI BstYI BstZ17I Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PshBI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfcI SmlI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XbaI XhoII XmnI