##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        ApaI        
AscI        Asp700I     Asp718I     AvaI        AviII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BcnI        BsaAI       BsiWI       BsoBI       BspDI       
BsrFI       BssHII      BstBI       BstEII      BstPI       
Cfr10I      ClaI        CpoI        DrdI        EagI        
EclXI       Eco52I      EcoO65I     FauI        FseI        
FspI        KasI        KpnI        KspI        MluI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SwaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII        4963  CGCG                 CG^CG
AcyI         1429  GGCGTC               G[GA]^CG[CT]C
AflII        5450  CTTAAG               C^TTAAG
AhdI         1263  GACGAGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      2935  AGCGCT               AGC^GCT
AseI         4284  ATTAAT               AT^TAAT
AsnI         4284  ATTAAT               AT^TAAT
AspEI        1263  GACGAGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1507  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        5523  CCTAGG               C^CTAGG
BanI          637  GGTGCC               G^G[CT][GA]CC
BclI         2607  TGATCA               T^GATCA
BfrI         5450  CTTAAG               C^TTAAG
BglI         2490  GCCTAGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         5523  CCTAGG               C^CTAGG
BsaHI        1429  GGCGTC               G[GA]^CG[CT]C
BsiEI        3101  CGGTCG               CG[GA][CT]^CG
BspHI        5288  TCATGA               T^CATGA
BsrBI        2813  CCGCTC               CCGCTC(-3/-3)
BssSI        4924  CTCGTG               CACGAG(-5/-1)
BstUI        4963  CGCG                 CG^CG
Eam1105I     1263  GACGAGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2935  AGCGCT               AGC^GCT
EcoNI        1556  CCTTGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        2666  GATATC               GAT^ATC
FbaI         2607  TGATCA               T^GATCA
HaeII        2935  AGCGCT               [GA]GCGC^[CT]
Hin1I        1429  GGCGTC               G[GA]^CG[CT]C
MfeI         2744  CAATTG               C^AATTG
MunI         2744  CAATTG               C^AATTG
MvnI         4963  CGCG                 CG^CG
PflFI        1507  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         841  CCAGGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1628  GGGTCCC              [GA]G^G[AT]CC[CT]
PshBI        4284  ATTAAT               AT^TAAT
RcaI         5288  TCATGA               T^CATGA
ScaI         1420  AGTACT               AGT^ACT
SpeI         5189  ACTAGT               A^CTAGT
Tth111I      1507  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        841  CCAGGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         3132  TCTAGA               T^CTAGA
XcmI         1902  CCAAGGAGTCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI         2559  GTATAC               GT^[AC][GT]AC
             3212  GTATAC              
AccIII        956  TCCGGA               T^CCGGA
             2772  TCCGGA              
AlwNI           3  CAGACGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2219  CAGGGACTG           
AvaII         807  GGTCC                G^G[AT]CC
             1629  GGTCC               
BalI          662  TGGCCA               TGG^CCA
             5104  TGGCCA              
BamHI        3532  GGATCC               G^GATCC
             3555  GGATCC              
BglII        4393  AGATCT               A^GATCT
             4567  AGATCT              
BsaBI        1203  GATGGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2076  GATTCCTATC          
BsaWI         956  TCCGGA               [AT]^CCGG[AT]
             2772  TCCGGA              
BseAI         956  TCCGGA               T^CCGGA
             2772  TCCGGA              
BseRI         347  GAGGAG               GAGGAG(10/8)
             2869  GAGGAG              
BspEI         956  TCCGGA               T^CCGGA
             2772  TCCGGA              
Bst1107I     2559  GTATAC               GTA^TAC
             3212  GTATAC              
BstXI        3487  CCAAGTGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5067  CCAAAGTGCTGG        
BstZ17I      2559  GTATAC               GTA^TAC
             3212  GTATAC              
DsaI          284  CCGTGG               C^C[GA][CT]GG
             2434  CCACGG              
EcoT22I      2801  ATGCAT               ATGCA^T
             3221  ATGCAT              
HapII         957  CCGG                 C^CGG
             2773  CCGG                
HpaI         2394  GTTAAC               GTT^AAC
             3279  GTTAAC              
HpaII         957  CCGG                 C^CGG
             2773  CCGG                
MamI         1203  GATGGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2076  GATTCCTATC          
MluNI         662  TGGCCA               TGG^CCA
             5104  TGGCCA              
MroI          956  TCCGGA               T^CCGGA
             2772  TCCGGA              
MscI          662  TGGCCA               TGG^CCA
             5104  TGGCCA              
MspI          957  CCGG                 C^CGG
             2773  CCGG                
NsiI         2801  ATGCAT               ATGCA^T
             3221  ATGCAT              
SacI          577  GAGCTC               GAGCT^C
             4519  GAGCTC              
SapI         1153  GAAGAGC              GCTCTTC(1/4)
             1999  GCTCTTC             


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
Alw44I      AlwI        AocI        ApaLI       ApoI        
AspHI       BanII       BbsI        BbvI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaI        BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
Bsp1286I    Bsp1407I    BspLU11I    BspMI       BsrDI       
BsrGI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      EaeI        EarI        Eco81I      
EcoO109I    EcoRI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HgaI        HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MvaI        MwoI        NdeI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspBI       
SspI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII