##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AgeI ApaI
AscI Asp700I Asp718I AvaI AviII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BsaAI BsiWI BsoBI BspDI
BsrFI BssHII BstBI BstEII BstPI
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco52I EcoO65I FauI FseI
FspI KasI KpnI KspI MluI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SwaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 4963 CGCG CG^CG
AcyI 1429 GGCGTC G[GA]^CG[CT]C
AflII 5450 CTTAAG C^TTAAG
AhdI 1263 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2935 AGCGCT AGC^GCT
AseI 4284 ATTAAT AT^TAAT
AsnI 4284 ATTAAT AT^TAAT
AspEI 1263 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1507 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5523 CCTAGG C^CTAGG
BanI 637 GGTGCC G^G[CT][GA]CC
BclI 2607 TGATCA T^GATCA
BfrI 5450 CTTAAG C^TTAAG
BglI 2490 GCCTAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 5523 CCTAGG C^CTAGG
BsaHI 1429 GGCGTC G[GA]^CG[CT]C
BsiEI 3101 CGGTCG CG[GA][CT]^CG
BspHI 5288 TCATGA T^CATGA
BsrBI 2813 CCGCTC CCGCTC(-3/-3)
BssSI 4924 CTCGTG CACGAG(-5/-1)
BstUI 4963 CGCG CG^CG
Eam1105I 1263 GACGAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2935 AGCGCT AGC^GCT
EcoNI 1556 CCTTGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2666 GATATC GAT^ATC
FbaI 2607 TGATCA T^GATCA
HaeII 2935 AGCGCT [GA]GCGC^[CT]
Hin1I 1429 GGCGTC G[GA]^CG[CT]C
MfeI 2744 CAATTG C^AATTG
MunI 2744 CAATTG C^AATTG
MvnI 4963 CGCG CG^CG
PflFI 1507 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 841 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1628 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 4284 ATTAAT AT^TAAT
RcaI 5288 TCATGA T^CATGA
ScaI 1420 AGTACT AGT^ACT
SpeI 5189 ACTAGT A^CTAGT
Tth111I 1507 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 841 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3132 TCTAGA T^CTAGA
XcmI 1902 CCAAGGAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2559 GTATAC GT^[AC][GT]AC
3212 GTATAC
AccIII 956 TCCGGA T^CCGGA
2772 TCCGGA
AlwNI 3 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
2219 CAGGGACTG
AvaII 807 GGTCC G^G[AT]CC
1629 GGTCC
BalI 662 TGGCCA TGG^CCA
5104 TGGCCA
BamHI 3532 GGATCC G^GATCC
3555 GGATCC
BglII 4393 AGATCT A^GATCT
4567 AGATCT
BsaBI 1203 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2076 GATTCCTATC
BsaWI 956 TCCGGA [AT]^CCGG[AT]
2772 TCCGGA
BseAI 956 TCCGGA T^CCGGA
2772 TCCGGA
BseRI 347 GAGGAG GAGGAG(10/8)
2869 GAGGAG
BspEI 956 TCCGGA T^CCGGA
2772 TCCGGA
Bst1107I 2559 GTATAC GTA^TAC
3212 GTATAC
BstXI 3487 CCAAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5067 CCAAAGTGCTGG
BstZ17I 2559 GTATAC GTA^TAC
3212 GTATAC
DsaI 284 CCGTGG C^C[GA][CT]GG
2434 CCACGG
EcoT22I 2801 ATGCAT ATGCA^T
3221 ATGCAT
HapII 957 CCGG C^CGG
2773 CCGG
HpaI 2394 GTTAAC GTT^AAC
3279 GTTAAC
HpaII 957 CCGG C^CGG
2773 CCGG
MamI 1203 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2076 GATTCCTATC
MluNI 662 TGGCCA TGG^CCA
5104 TGGCCA
MroI 956 TCCGGA T^CCGGA
2772 TCCGGA
MscI 662 TGGCCA TGG^CCA
5104 TGGCCA
MspI 957 CCGG C^CGG
2773 CCGG
NsiI 2801 ATGCAT ATGCA^T
3221 ATGCAT
SacI 577 GAGCTC GAGCT^C
4519 GAGCTC
SapI 1153 GAAGAGC GCTCTTC(1/4)
1999 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AocI ApaLI ApoI
AspHI BanII BbsI BbvI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII EaeI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HgaI HhaI HinP1I
HincII HindII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII