##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AscI AseI AsnI Asp718I
AviII BbeI BcgI BciVI BsaBI
BsaHI BseAI BsiEI BspDI BspEI
BspHI BsrFI BssHII Bst1107I BstBI
BstUI BstZ17I Cfr10I ClaI CpoI
DrdI EagI EclXI Eco52I EcoRV
FseI FspI HgaI Hin1I HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshBI
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SpeI Sse8387I StuI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AclI 3908 AACGTT AA^CGTT
AflII 3027 CTTAAG C^TTAAG
Alw44I 575 GTGCAC G^TGCAC
Aor51HI 2791 AGCGCT AGC^GCT
ApaI 2344 GGGCCC GGGCC^C
ApaLI 575 GTGCAC G^TGCAC
Asp700I 2367 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3072 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 965 CTCGGG C^[CT]CG[GA]G
AvrII 24 CCTAGG C^CTAGG
BamHI 242 GGATCC G^GATCC
BanI 4396 GGCACC G^G[CT][GA]CC
BbrPI 2204 CACGTG CAC^GTG
BfrI 3027 CTTAAG C^TTAAG
BglI 1899 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 24 CCTAGG C^CTAGG
BsaI 1768 GAGACC GGTCTC(1/5)
BsiWI 4267 CGTACG C^GTACG
BsoBI 965 CTCGGG C^[CT]CG[GA]G
BspLU11I 5325 ACATGT A^CATGT
BssSI 2691 CACGAG CACGAG(-5/-1)
BstXI 154 CCAGCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eco47III 2791 AGCGCT AGC^GCT
EcoT22I 3360 ATGCAT ATGCA^T
HaeII 2791 AGCGCT [GA]GCGC^[CT]
HincII 1749 GTTGAC GT[CT]^[GA]AC
HindII 1749 GTTGAC GT[CT]^[GA]AC
HindIII 192 AAGCTT A^AGCTT
MfeI 4361 CAATTG C^AATTG
MunI 4361 CAATTG C^AATTG
NsiI 3360 ATGCAT ATGCA^T
PflFI 3072 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2204 CACGTG CAC^GTG
PmlI 2204 CACGTG CAC^GTG
PshAI 5324 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3908 AACGTT AA^CGTT
SnaBI 942 TACGTA TAC^GTA
SphI 2056 GCATGC GCATG^C
TaqI 2267 TCGA T^CGA
Tth111I 3072 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2267 TCGA T^CGA
XmnI 2367 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 2538 GTAGAC GT^[AC][GT]AC
3849 GTAGAC
AflIII 2203 ACACGT A^C[GA][CT]GT
5325 ACATGT
AhdI 120 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5118 GACATGCTGTC
AocI 1641 CCTGAGG CC^T[ACGT]AGG
3420 CCTAAGG
AspEI 120 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5118 GACATGCTGTC
BclI 476 TGATCA T^GATCA
4101 TGATCA
BlpI 93 GCTCAGC GC^T[ACGT]AGC
776 GCTGAGC
Bpu1102I 93 GCTCAGC GC^T[ACGT]AGC
776 GCTGAGC
BsaWI 1666 TCCGGT [AT]^CCGG[AT]
4468 ACCGGA
BsmBI 204 GAGACG CGTCTC(1/5)
3900 GAGACG
BsrBI 4340 CCGCTC CCGCTC(-3/-3)
594 GAGCGG
BstAPI 1322 GCACAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1418 GCAGCCACTGC
Bsu36I 1641 CCTGAGG CC^T[ACGT]AGG
3420 CCTAAGG
CelII 93 GCTCAGC GC^T[ACGT]AGC
776 GCTGAGC
DraI 3455 TTTAAA TTT^AAA
5372 TTTAAA
DraIII 1323 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4524 CACAGAGTG
Eam1105I 120 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5118 GACATGCTGTC
Eco81I 1641 CCTGAGG CC^T[ACGT]AGG
3420 CCTAAGG
EcoNI 2147 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2354 CCTGTGTGAGG
FbaI 476 TGATCA T^GATCA
4101 TGATCA
SacI 3298 GAGCTC GAGCT^C
4430 GAGCTC
ScaI 895 AGTACT AGT^ACT
3031 AGTACT
SspI 1105 AATATT AAT^ATT
5298 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanII BbsI BbvCI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoO65I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII