##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
Asp718I BclI BfrI BsiWI BsmI
Bsp1407I BspDI BsrGI Bst1107I BstAPI
BstZ17I ClaI CpoI DraI DraIII
EcoRV EcoT22I FbaI HincII HindII
HpaI KpnI KspI MfeI MluI
MunI NheI NotI NruI NsiI
PacI PaeR7I PinAI PmeI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 5316 GACGTC GACGT^C
AccI 2498 GTCTAC GT^[AC][GT]AC
AocI 4771 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2460 AGCGCT AGC^GCT
AseI 2128 ATTAAT AT^TAAT
AsnI 2128 ATTAAT AT^TAAT
Asp700I 2041 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 356 TGCGCA TGC^GCA
BbeI 4893 GGCGCC GGCGC^C
BcgI 4832 GCAGGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 626 GTATCC GTATCC(6/5)
BsaBI 1354 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 3246 TCATGA T^CATGA
BspLU11I 2065 ACATGT A^CATGT
BstBI 2447 TTCGAA TT^CGAA
Bsu36I 4771 CCTGAGG CC^T[ACGT]AGG
Eco47III 2460 AGCGCT AGC^GCT
Eco81I 4771 CCTGAGG CC^T[ACGT]AGG
EcoRI 4671 GAATTC G^AATTC
FseI 4457 GGCCGGCC GGCCGG^CC
FspI 356 TGCGCA TGC^GCA
KasI 4893 GGCGCC G^GCGCC
MamI 1354 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 4893 GGCGCC GG^CGCC
NdeI 1346 CATATG CA^TATG
NspV 2447 TTCGAA TT^CGAA
PshBI 2128 ATTAAT AT^TAAT
RcaI 3246 TCATGA T^CATGA
SfoI 4893 GGCGCC GGC^GCC
SfuI 2447 TTCGAA TT^CGAA
SgrAI 2600 CGCCGGTG C[GA]^CCGG[CT]G
XbaI 4675 TCTAGA T^CTAGA
XmnI 2041 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AhdI 2088 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3278 GACATCTAGTC
ApaI 3419 GGGCCC GGGCC^C
3484 GGGCCC
AspEI 2088 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3278 GACATCTAGTC
BbrPI 382 CACGTG CAC^GTG
4444 CACGTG
BbvCI 1883 CCTCAGC CCTCAGC(-5/-2)
3791 GCTGAGG
BssHII 35 GCGCGC G^CGCGC
4182 GCGCGC
BstEII 3348 GGTGACC G^GT[ACGT]ACC
3989 GGTCACC
BstPI 3348 GGTGACC G^GT[ACGT]ACC
3989 GGTCACC
Eam1105I 2088 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3278 GACATCTAGTC
EarI 1505 CTCTTC CTCTTC(1/4)
4780 CTCTTC
EcoNI 947 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5432 CCTTGAGTAGG
EcoO65I 3348 GGTGACC G^GT[ACGT]ACC
3989 GGTCACC
HindIII 5068 AAGCTT A^AGCTT
5520 AAGCTT
Ksp632I 1505 CTCTTC CTCTTC(1/4)
4780 CTCTTC
PmaCI 382 CACGTG CAC^GTG
4444 CACGTG
PmlI 382 CACGTG CAC^GTG
4444 CACGTG
PstI 496 CTGCAG CTGCA^G
1159 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaLI ApoI AspHI AspI
AvaI AvaII AvrII BalI BamHI
BanI BanII BbsI BbvI BcnI
BfaI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PshAI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI