##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AlwNI Aor51HI ApaI
AscI Asp718I BbeI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspHI BsrBI BsrFI BssHII
Bst1107I BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoO65I FauI FseI
Hin1I HpaI KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NgoMIV NheI NotI NruI
PacI PinAI PmeI PshAI Psp1406I
PvuI RcaI RsrII SacII SapI
SbfI SexAI SfiI SfoI SgrAI
SnaBI Sse8387I XcmI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 91 GTCGAC GT^[AC][GT]AC
AflIII 4874 ACATGT A^C[GA][CT]GT
Alw44I 3850 GTGCAC G^TGCAC
AocI 1600 CCTGAGG CC^T[ACGT]AGG
ApaLI 3850 GTGCAC G^TGCAC
AspI 3110 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4196 TGGCCA TGG^CCA
BamHI 3525 GGATCC G^GATCC
BbrPI 5458 CACGTG CAC^GTG
BglII 816 AGATCT A^GATCT
BsaAI 5458 CACGTG [CT]AC^GT[GA]
BseRI 3325 GAGGAG GAGGAG(10/8)
BsmBI 2849 GAGACG CGTCTC(1/5)
Bsp1407I 5688 TGTACA T^GTACA
BspLU11I 4874 ACATGT A^CATGT
BsrGI 5688 TGTACA T^GTACA
BssSI 2276 CACGAG CACGAG(-5/-1)
BstAPI 1994 GCACCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2630 TTCGAA TT^CGAA
Bsu36I 1600 CCTGAGG CC^T[ACGT]AGG
DraIII 1947 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 850 GACCTATTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 4196 TGGCCA [CT]^GGCC[GA]
Eco81I 1600 CCTGAGG CC^T[ACGT]AGG
EcoNI 4394 CCTTTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3507 GATATC GAT^ATC
EcoT22I 5903 ATGCAT ATGCA^T
HindIII 1304 AAGCTT A^AGCTT
MfeI 5612 CAATTG C^AATTG
MluNI 4196 TGGCCA TGG^CCA
MscI 4196 TGGCCA TGG^CCA
MunI 5612 CAATTG C^AATTG
NdeI 4077 CATATG CA^TATG
NsiI 5903 ATGCAT ATGCA^T
NspV 2630 TTCGAA TT^CGAA
PaeR7I 5222 CTCGAG C^TCGAG
PflFI 3110 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2547 CCACTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 5458 CACGTG CAC^GTG
PmlI 5458 CACGTG CAC^GTG
PvuII 4639 CAGCTG CAG^CTG
SalI 91 GTCGAC G^TCGAC
SfuI 2630 TTCGAA TT^CGAA
SmaI 3618 CCCGGG CCC^GGG
SpeI 1554 ACTAGT A^CTAGT
SspBI 5688 TGTACA T^GTACA
StuI 2438 AGGCCT AGG^CCT
Tth111I 3110 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2547 CCACTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 5222 CTCGAG C^TCGAG
XmaCI 3618 CCCGGG C^CCGGG
XmaI 3618 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflII 3559 CTTAAG C^TTAAG
5530 CTTAAG
AhdI 215 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2799 GACCTGAAGTC
AseI 3085 ATTAAT AT^TAAT
5289 ATTAAT
AsnI 3085 ATTAAT AT^TAAT
5289 ATTAAT
AspEI 215 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2799 GACCTGAAGTC
AviII 158 TGCGCA TGC^GCA
2061 TGCGCA
BbvCI 2030 CCTCAGC CCTCAGC(-5/-2)
5244 GCTGAGG
BcgI 4638 GCAGCTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5000 GCAATGGTATCG
BfrI 3559 CTTAAG C^TTAAG
5530 CTTAAG
BglI 40 GCCTCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2003 GCCTTCCAGGC
BlpI 1956 GCTCAGC GC^T[ACGT]AGC
3944 GCTTAGC
Bpu1102I 1956 GCTCAGC GC^T[ACGT]AGC
3944 GCTTAGC
CelII 1956 GCTCAGC GC^T[ACGT]AGC
3944 GCTTAGC
DsaI 2299 CCATGG C^C[GA][CT]GG
2311 CCATGG
Eam1105I 215 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2799 GACCTGAAGTC
FspI 158 TGCGCA TGC^GCA
2061 TGCGCA
HaeII 2541 AGCGCC [GA]GCGC^[CT]
5779 GGCGCT
HincII 91 GTCGAC GT[CT]^[GA]AC
4663 GTTGAC
HindII 91 GTCGAC GT[CT]^[GA]AC
4663 GTTGAC
MspA1I 3514 CCGCTG C[AC]G^C[GT]G
4639 CAGCTG
NcoI 2299 CCATGG C^CATGG
2311 CCATGG
PshBI 3085 ATTAAT AT^TAAT
5289 ATTAAT
SacI 2144 GAGCTC GAGCT^C
2594 GAGCTC
ScaI 1461 AGTACT AGT^ACT
4419 AGTACT
SwaI 3747 ATTTAAAT ATTT^AAAT
5565 ATTTAAAT
XbaI 3550 TCTAGA T^CTAGA
5033 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaI AvaII
AvrII BanI BanII BbsI BbvI
BciVI BclI BcnI BfaI BlnI
BmyI BpmI BpuAI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI