##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII AclI AgeI AscI AseI
AsnI AviII BciVI BsaBI BsiWI
Bst1107I BstZ17I CpoI DrdI EcoRV
FspI HincII HindII HpaI MamI
MluI NdeI NheI NotI NruI
PacI PaeR7I PflMI PinAI PmeI
PshBI Psp1406I RsrII SalI SapI
SbfI ScaI SexAI SfiI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 956 GGTACC G^GTACC
AccIII 1836 TCCGGA T^CCGGA
AflII 652 CTTAAG C^TTAAG
AhdI 4357 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3720 GTGCAC G^TGCAC
Aor51HI 2783 AGCGCT AGC^GCT
ApaLI 3720 GTGCAC G^TGCAC
Asp718I 956 GGTACC G^GTACC
AspEI 4357 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3750 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4994 CCTAGG C^CTAGG
BamHI 3870 GGATCC G^GATCC
BbrPI 4167 CACGTG CAC^GTG
BbsI 1740 GTCTTC GAAGAC(2/6)
BfrI 652 CTTAAG C^TTAAG
BglI 346 GCCTTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4994 CCTAGG C^CTAGG
BlpI 4066 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4066 GCTGAGC GC^T[ACGT]AGC
BpuAI 1740 GTCTTC GAAGAC(2/6)
BseAI 1836 TCCGGA T^CCGGA
BspDI 2357 ATCGAT AT^CGAT
BspEI 1836 TCCGGA T^CCGGA
BsrBI 4799 CCGCTC CCGCTC(-3/-3)
BssSI 1210 CTCGTG CACGAG(-5/-1)
BstAPI 3959 GCAAGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2690 TTCGAA TT^CGAA
BstEII 904 GGTCACC G^GT[ACGT]ACC
BstPI 904 GGTCACC G^GT[ACGT]ACC
CelII 4066 GCTGAGC GC^T[ACGT]AGC
ClaI 2357 ATCGAT AT^CGAT
DraIII 1872 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 145 CGGCCG C^GGCCG
Eam1105I 4357 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 145 CGGCCG C^GGCCG
Eco47III 2783 AGCGCT AGC^GCT
Eco52I 145 CGGCCG C^GGCCG
EcoO65I 904 GGTCACC G^GT[ACGT]ACC
EcoRI 841 GAATTC G^AATTC
EcoT22I 1019 ATGCAT ATGCA^T
FseI 3778 GGCCGGCC GGCCGG^CC
HindIII 4521 AAGCTT A^AGCTT
KpnI 956 GGTACC GGTAC^C
MfeI 4496 CAATTG C^AATTG
MroI 1836 TCCGGA T^CCGGA
MunI 4496 CAATTG C^AATTG
NcoI 317 CCATGG C^CATGG
NsiI 1019 ATGCAT ATGCA^T
NspV 2690 TTCGAA TT^CGAA
PflFI 3750 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4167 CACGTG CAC^GTG
PmlI 4167 CACGTG CAC^GTG
PvuI 580 CGATCG CGAT^CG
SfuI 2690 TTCGAA TT^CGAA
SpeI 2481 ACTAGT A^CTAGT
Tth111I 3750 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4034 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 523 GTAGAC GT^[AC][GT]AC
795 GTCTAC
AocI 1783 CCTCAGG CC^T[ACGT]AGG
4005 CCTTAGG
Asp700I 3201 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3561 GAAGGGCTTC
BalI 285 TGGCCA TGG^CCA
401 TGGCCA
BcgI 3504 CGAGTGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3652 CGACGGGCTTGC
BglII 3977 AGATCT A^GATCT
4671 AGATCT
BsmBI 2976 CGTCTC CGTCTC(1/5)
2833 GAGACG
BspLU11I 2128 ACATGT A^CATGT
4739 ACATGT
BsrFI 3473 GCCGGC [GA]^CCGG[CT]
3779 GCCGGC
BstXI 690 CCAGGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3127 CCAGGTGAATGG
Bsu36I 1783 CCTCAGG CC^T[ACGT]AGG
4005 CCTTAGG
Cfr10I 3473 GCCGGC [GA]^CCGG[CT]
3779 GCCGGC
Eco81I 1783 CCTCAGG CC^T[ACGT]AGG
4005 CCTTAGG
EcoNI 1124 CCTATCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3366 CCTCTGCAAGG
KspI 240 CCGCGG CCGC^GG
446 CCGCGG
MluNI 285 TGGCCA TGG^CCA
401 TGGCCA
MscI 285 TGGCCA TGG^CCA
401 TGGCCA
NaeI 3473 GCCGGC GCC^GGC
3779 GCCGGC
NgoMIV 3473 GCCGGC G^CCGGC
3779 GCCGGC
PpuMI 160 AGGTCCC [GA]G^G[AT]CC[CT]
4346 AGGTCCT
PshAI 2835 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3311 GACCCTGGTC
SacII 240 CCGCGG CCGC^GG
446 CCGCGG
StuI 2574 AGGCCT AGG^CCT
3152 AGGCCT
XcmI 951 CCAGCGGTACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2872 CCAGGGATAGCTTGG
XmnI 3201 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3561 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbeI BbvCI BbvI BclI
BcnI BfaI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrGI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII