##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccIII AgeI AhdI AocI
ApaI AscI AseI AsnI AspEI
AspI AviII BbeI BbrPI BbvCI
BcgI BlpI Bpu1102I BsaAI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BsrBI BssHII BssSI BstBI Bsu36I
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoT22I FseI FspI KasI
KspI MluI MroI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI PvuI
RcaI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 4652 GGTACC G^GTACC
AccII 168 CGCG CG^CG
AclI 3405 AACGTT AA^CGTT
AcyI 2859 GGCGTC G[GA]^CG[CT]C
AflII 2880 CTTAAG C^TTAAG
Aor51HI 195 AGCGCT AGC^GCT
Asp718I 4652 GGTACC G^GTACC
AvrII 5063 CCTAGG C^CTAGG
BamHI 1960 GGATCC G^GATCC
BfrI 2880 CTTAAG C^TTAAG
BglI 1778 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2968 AGATCT A^GATCT
BlnI 5063 CCTAGG C^CTAGG
BsaHI 2859 GGCGTC G[GA]^CG[CT]C
BsaWI 265 TCCGGT [AT]^CCGG[AT]
BsmBI 2861 CGTCTC CGTCTC(1/5)
BsrDI 555 GCAATG GCAATG(2/0)
BstAPI 5596 GCATAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1594 GGTGACC G^GT[ACGT]ACC
BstPI 1594 GGTGACC G^GT[ACGT]ACC
BstUI 168 CGCG CG^CG
Eco47III 195 AGCGCT AGC^GCT
EcoO65I 1594 GGTGACC G^GT[ACGT]ACC
EcoRI 1887 GAATTC G^AATTC
EcoRV 3208 GATATC GAT^ATC
HaeII 195 AGCGCT [GA]GCGC^[CT]
HgaI 2860 GCGTC GACGC(5/10)
Hin1I 2859 GGCGTC G[GA]^CG[CT]C
KpnI 4652 GGTACC GGTAC^C
MfeI 1814 CAATTG C^AATTG
MunI 1814 CAATTG C^AATTG
MvnI 168 CGCG CG^CG
NdeI 5487 CATATG CA^TATG
PaeR7I 4887 CTCGAG C^TCGAG
Psp1406I 3405 AACGTT AA^CGTT
SacI 3676 GAGCTC GAGCT^C
SexAI 3806 ACCTGGT A^CC[AT]GGT
StuI 744 AGGCCT AGG^CCT
SwaI 2024 ATTTAAAT ATTT^AAAT
XbaI 49 TCTAGA T^CTAGA
XhoI 4887 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Asp700I 3296 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3994 GAAATGCTTC
BalI 1386 TGGCCA TGG^CCA
2225 TGGCCA
BciVI 69 GTATCC GTATCC(6/5)
86 GTATCC
BclI 1032 TGATCA T^GATCA
4328 TGATCA
BcnI 17 CCGGG CC^[GC]GG
129 CCCGG
BsaBI 2261 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3208 GATATCAATC
BsaI 3667 GAGACC GGTCTC(1/5)
241 GGTCTC
BsmI 2157 GAATGC GAATGC(1/-1)
449 GCATTC
Bsp1407I 738 TGTACA T^GTACA
1688 TGTACA
BsrGI 738 TGTACA T^GTACA
1688 TGTACA
DsaI 4292 CCACGG C^C[GA][CT]GG
5519 CCGTGG
FauI 21 GCGGG CCCGC(4/6)
3873 GCGGG
FbaI 1032 TGATCA T^GATCA
4328 TGATCA
HindIII 2117 AAGCTT A^AGCTT
5290 AAGCTT
HpaI 2610 GTTAAC GTT^AAC
4160 GTTAAC
MamI 2261 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3208 GATATCAATC
MluNI 1386 TGGCCA TGG^CCA
2225 TGGCCA
MscI 1386 TGGCCA TGG^CCA
2225 TGGCCA
NciI 17 CCGGG CC^[GC]GG
129 CCCGG
PflMI 3374 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4640 CCACCTCCTGG
PpuMI 884 GGGACCT [GA]G^G[AT]CC[CT]
2002 AGGTCCT
SspBI 738 TGTACA T^GTACA
1688 TGTACA
TaqI 1800 TCGA T^CGA
4888 TCGA
TthHB8I 1800 TCGA T^CGA
4888 TCGA
Van91I 3374 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4640 CCACCTCCTGG
XcmI 316 CCAAAAGGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2499 CCACAAAGACTGTGG
XmnI 3296 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3994 GAAATGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BspMI BsrFI
BsrI BssKI Bst1107I BstF5I BstNI
BstXI BstYI BstZ17I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshAI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII