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# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII AccIII AclI AgeI AocI
AscI AvrII BlnI BseAI BsiWI
BspDI BspEI BstEII BstPI Bsu36I
ClaI CpoI Eco81I EcoNI EcoO65I
EcoRV EcoT22I FseI HindIII HpaI
MroI NdeI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
Psp1406I RsrII SalI ScaI SfiI
SgrAI SnaBI SpeI SwaI XhoI
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# ONE CUT ENZYMES ( 69 enzymes) #
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AcyI 1 GGCGCC G[GA]^CG[CT]C
AflII 1475 CTTAAG C^TTAAG
AhdI 4024 GACGGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3163 AGCGCT AGC^GCT
AseI 4510 ATTAAT AT^TAAT
AsnI 4510 ATTAAT AT^TAAT
AspEI 4024 GACGGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2466 TGCGCA TGC^GCA
BbeI 1 GGCGCC GGCGC^C
BbrPI 1725 CACGTG CAC^GTG
BbvCI 2001 GCTGAGG CCTCAGC(-5/-2)
BcgI 289 CGAATCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1154 GGATAC GTATCC(6/5)
BfrI 1475 CTTAAG C^TTAAG
BglII 3782 AGATCT A^GATCT
BlpI 268 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 268 GCTCAGC GC^T[ACGT]AGC
BsaBI 4152 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1 GGCGCC G[GA]^CG[CT]C
BsaWI 2378 TCCGGT [AT]^CCGG[AT]
BsmI 2787 GAATGC GAATGC(1/-1)
Bsp1407I 4002 TGTACA T^GTACA
BspHI 3068 TCATGA T^CATGA
BsrBI 3326 CCGCTC CCGCTC(-3/-3)
BsrDI 1783 GCAATG GCAATG(2/0)
BsrGI 4002 TGTACA T^GTACA
BssSI 446 CACGAG CACGAG(-5/-1)
Bst1107I 3355 GTATAC GTA^TAC
BstBI 1441 TTCGAA TT^CGAA
BstZ17I 3355 GTATAC GTA^TAC
CelII 268 GCTCAGC GC^T[ACGT]AGC
DrdI 3506 GACCCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4024 GACGGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3163 AGCGCT AGC^GCT
FspI 2466 TGCGCA TGC^GCA
HgaI 66 GACGC GACGC(5/10)
Hin1I 1 GGCGCC G[GA]^CG[CT]C
HincII 2857 GTCAAC GT[CT]^[GA]AC
HindII 2857 GTCAAC GT[CT]^[GA]AC
KasI 1 GGCGCC G^GCGCC
MamI 4152 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1858 CAATTG C^AATTG
MluI 67 ACGCGT A^CGCGT
MunI 1858 CAATTG C^AATTG
NaeI 2754 GCCGGC GCC^GGC
NarI 1 GGCGCC GG^CGCC
NgoMIV 2754 GCCGGC G^CCGGC
NspV 1441 TTCGAA TT^CGAA
PflMI 1943 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1725 CACGTG CAC^GTG
PmlI 1725 CACGTG CAC^GTG
PpuMI 3766 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2245 GACACCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4510 ATTAAT AT^TAAT
PvuI 3640 CGATCG CGAT^CG
RcaI 3068 TCATGA T^CATGA
SacI 207 GAGCTC GAGCT^C
SapI 209 GCTCTTC GCTCTTC(1/4)
SbfI 245 CCTGCAGG CCTGCA^GG
SexAI 2221 ACCAGGT A^CC[AT]GGT
SfoI 1 GGCGCC GGC^GCC
SfuI 1441 TTCGAA TT^CGAA
Sse8387I 245 CCTGCAGG CCTGCA^GG
SspBI 4002 TGTACA T^GTACA
SspI 4513 AATATT AAT^ATT
StuI 1865 AGGCCT AGG^CCT
Van91I 1943 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4877 TCTAGA T^CTAGA
XcmI 3086 CCAGACTCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 2476 GGTACC G^GTACC
3081 GGTACC
Alw44I 1743 GTGCAC G^TGCAC
2771 GTGCAC
ApaI 1229 GGGCCC GGGCC^C
2968 GGGCCC
ApaLI 1743 GTGCAC G^TGCAC
2771 GTGCAC
Asp718I 2476 GGTACC G^GTACC
3081 GGTACC
BfaI 838 CTAG C^TAG
4878 CTAG
BsaI 958 GAGACC GGTCTC(1/5)
1272 GAGACC
BsmBI 1977 GAGACG CGTCTC(1/5)
3636 GAGACG
BspLU11I 2407 ACATGT A^CATGT
4610 ACATGT
BssHII 524 GCGCGC G^CGCGC
546 GCGCGC
BstAPI 1667 GCACTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1926 GCAGGTGCTGC
DraIII 1692 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2917 CACCCTGTG
EagI 92 CGGCCG C^GGCCG
129 CGGCCG
EclXI 92 CGGCCG C^GGCCG
129 CGGCCG
Eco52I 92 CGGCCG C^GGCCG
129 CGGCCG
EcoRI 3647 GAATTC G^AATTC
3901 GAATTC
KpnI 2476 GGTACC GGTAC^C
3081 GGTACC
KspI 6 CCGCGG CCGC^GG
187 CCGCGG
MaeI 838 CTAG C^TAG
4878 CTAG
SacII 6 CCGCGG CCGC^GG
187 CCGCGG
SmaI 1967 CCCGGG CCC^GGG
3205 CCCGGG
SphI 1595 GCATGC GCATG^C
1766 GCATGC
XmaCI 1967 CCCGGG C^CCGGG
3205 CCCGGG
XmaI 1967 CCCGGG C^CCGGG
3205 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvI BclI BcnI BglI BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII XmnI