##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccI AcyI AgeI Aor51HI
ApaI AscI AspI AvaI AviII
BbeI BclI BglI BglII BlpI
Bpu1102I BsaHI BsiWI BsoBI BspDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI Hin1I
HpaI KasI MfeI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PaeR7I PflFI PinAI
PmeI PshAI PvuI RsrII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 2313 GGTACC G^GTACC
AccIII 2536 TCCGGA T^CCGGA
AclI 420 AACGTT AA^CGTT
AhdI 3971 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1400 GTGCAC G^TGCAC
ApaLI 1400 GTGCAC G^TGCAC
AseI 2689 ATTAAT AT^TAAT
AsnI 2689 ATTAAT AT^TAAT
Asp718I 2313 GGTACC G^GTACC
AspEI 3971 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1108 GGATCC G^GATCC
BbrPI 2225 CACGTG CAC^GTG
BbsI 5006 GTCTTC GAAGAC(2/6)
BcgI 142 CGAGCCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 5006 GTCTTC GAAGAC(2/6)
BsaWI 2536 TCCGGA [AT]^CCGG[AT]
BseAI 2536 TCCGGA T^CCGGA
BsmBI 3476 GAGACG CGTCTC(1/5)
BsmI 926 GCATTC GAATGC(1/-1)
BspEI 2536 TCCGGA T^CCGGA
BspHI 1386 TCATGA T^CATGA
BsrBI 89 CCGCTC CCGCTC(-3/-3)
BssSI 40 CTCGTG CACGAG(-5/-1)
DraI 5394 TTTAAA TTT^AAA
DrdI 76 GACCCGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3971 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 3566 ATGCAT ATGCA^T
HaeII 287 GGCGCT [GA]GCGC^[CT]
KpnI 2313 GGTACC GGTAC^C
KspI 57 CCGCGG CCGC^GG
MluI 467 ACGCGT A^CGCGT
MroI 2536 TCCGGA T^CCGGA
NsiI 3566 ATGCAT ATGCA^T
PacI 5449 TTAATTAA TTAAT^TAA
PflMI 4597 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2225 CACGTG CAC^GTG
PmlI 2225 CACGTG CAC^GTG
PshBI 2689 ATTAAT AT^TAAT
Psp1406I 420 AACGTT AA^CGTT
PvuII 3063 CAGCTG CAG^CTG
RcaI 1386 TCATGA T^CATGA
SacII 57 CCGCGG CCGC^GG
SapI 3319 GAAGAGC GCTCTTC(1/4)
SspI 3646 AATATT AAT^ATT
Van91I 4597 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflII 1282 CTTAAG C^TTAAG
1507 CTTAAG
AocI 3557 CCTAAGG CC^T[ACGT]AGG
4898 CCTCAGG
Asp700I 1414 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3644 GAAATATTTC
AvrII 2797 CCTAGG C^CTAGG
4468 CCTAGG
BalI 2950 TGGCCA TGG^CCA
4542 TGGCCA
BbvCI 4586 CCTCAGC CCTCAGC(-5/-2)
4782 CCTCAGC
BfrI 1282 CTTAAG C^TTAAG
1507 CTTAAG
BlnI 2797 CCTAGG C^CTAGG
4468 CCTAGG
BsaAI 2225 CACGTG [CT]AC^GT[GA]
5212 CACGTA
BsaI 2639 GAGACC GGTCTC(1/5)
4552 GGTCTC
BsgI 210 GTGCAG GTGCAG(16/14)
4728 GTGCAG
BsiEI 201 CGACCG CG[GA][CT]^CG
3216 CGACCG
BspLU11I 916 ACATGT A^CATGT
4376 ACATGT
BsrDI 378 CATTGC GCAATG(2/0)
4722 GCAATG
BstXI 713 CCAAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4926 CCAAAGTGCTGG
Bsu36I 3557 CCTAAGG CC^T[ACGT]AGG
4898 CCTCAGG
EaeI 2950 TGGCCA [CT]^GGCC[GA]
4542 TGGCCA
Eco81I 3557 CCTAAGG CC^T[ACGT]AGG
4898 CCTCAGG
HindIII 3464 AAGCTT A^AGCTT
3929 AAGCTT
MluNI 2950 TGGCCA TGG^CCA
4542 TGGCCA
MscI 2950 TGGCCA TGG^CCA
4542 TGGCCA
NcoI 1447 CCATGG C^CATGG
2577 CCATGG
PpuMI 297 GGGTCCT [GA]G^G[AT]CC[CT]
2068 AGGTCCC
SacI 641 GAGCTC GAGCT^C
1781 GAGCTC
SpeI 2489 ACTAGT A^CTAGT
4500 ACTAGT
XmnI 1414 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3644 GAAATATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbvI BciVI
BcnI BfaI BmyI BpmI BsaBI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScaI ScrFI
SexAI SfaNI SfcI SmlI SphI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII