##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AviII BamHI BbeI BbrPI
BglI BlpI Bpu1102I BsaBI BsiEI
BsiWI BspDI BspLU11I BssHII BstAPI
BstBI BstEII BstPI CelII ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FseI FspI KasI KpnI
KspI MamI MluI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PpuMI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SmaI SnaBI SpeI
Sse8387I SwaI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 3202 CGCG CG^CG
AccIII 3397 TCCGGA T^CCGGA
AclI 3538 AACGTT AA^CGTT
AcyI 3268 GACGCC G[GA]^CG[CT]C
AflII 1863 CTTAAG C^TTAAG
AocI 1255 CCTCAGG CC^T[ACGT]AGG
AseI 2562 ATTAAT AT^TAAT
AsnI 2562 ATTAAT AT^TAAT
AvrII 2837 CCTAGG C^CTAGG
BanI 1573 GGTGCC G^G[CT][GA]CC
BbvCI 164 CCTCAGC CCTCAGC(-5/-2)
BcgI 567 GCATCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1759 GTATCC GTATCC(6/5)
BfrI 1863 CTTAAG C^TTAAG
BglII 1800 AGATCT A^GATCT
BlnI 2837 CCTAGG C^CTAGG
BsaHI 3268 GACGCC G[GA]^CG[CT]C
BseAI 3397 TCCGGA T^CCGGA
BsmBI 34 GAGACG CGTCTC(1/5)
Bsp1407I 829 TGTACA T^GTACA
BspEI 3397 TCCGGA T^CCGGA
BsrBI 11 GAGCGG CCGCTC(-3/-3)
BsrDI 1818 GCAATG GCAATG(2/0)
BsrFI 1339 ACCGGC [GA]^CCGG[CT]
BsrGI 829 TGTACA T^GTACA
BssSI 1057 CACGAG CACGAG(-5/-1)
BstUI 3202 CGCG CG^CG
BstXI 4182 CCAAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1255 CCTCAGG CC^T[ACGT]AGG
CfoI 705 GCGC GCG^C
Cfr10I 1339 ACCGGC [GA]^CCGG[CT]
DraII 465 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eco81I 1255 CCTCAGG CC^T[ACGT]AGG
EcoNI 1244 CCTCAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 465 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 412 GATATC GAT^ATC
FauI 620 CCCGC CCCGC(4/6)
HaeII 704 GGCGCT [GA]GCGC^[CT]
HhaI 705 GCGC GCG^C
Hin1I 3268 GACGCC G[GA]^CG[CT]C
HinP1I 705 GCGC G^CGC
MroI 3397 TCCGGA T^CCGGA
MvnI 3202 CGCG CG^CG
NcoI 2887 CCATGG C^CATGG
NdeI 1126 CATATG CA^TATG
PaeR7I 1854 CTCGAG C^TCGAG
PshAI 2133 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2562 ATTAAT AT^TAAT
Psp1406I 3538 AACGTT AA^CGTT
PstI 1509 CTGCAG CTGCA^G
SacI 974 GAGCTC GAGCT^C
SgrAI 1338 CACCGGCG C[GA]^CCGG[CT]G
SphI 652 GCATGC GCATG^C
SspBI 829 TGTACA T^GTACA
XhoI 1854 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 5183 ACACGT A^C[GA][CT]GT
5191 ACGTGT
Asp700I 262 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3011 GAATAGTTTC
AspI 528 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1393 GACAGCGTC
AvaI 1142 CCCGAG C^[CT]CG[GA]G
1854 CTCGAG
AvaII 1462 GGACC G^G[AT]CC
3044 GGACC
BsaAI 5184 CACGTA [CT]AC^GT[GA]
5190 TACGTG
BsaI 1404 GAGACC GGTCTC(1/5)
3090 GAGACC
BsoBI 1142 CCCGAG C^[CT]CG[GA]G
1854 CTCGAG
Bst1107I 5179 GTATAC GTA^TAC
5187 GTATAC
BstZ17I 5179 GTATAC GTA^TAC
5187 GTATAC
DsaI 1468 CCGTGG C^C[GA][CT]GG
2887 CCATGG
HpaI 719 GTTAAC GTT^AAC
2517 GTTAAC
MfeI 2135 CAATTG C^AATTG
5427 CAATTG
MunI 2135 CAATTG C^AATTG
5427 CAATTG
NspI 652 GCATGC [GA]CATG^[CT]
5011 GCATGT
PflFI 528 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1393 GACAGCGTC
SapI 57 GAAGAGC GCTCTTC(1/4)
424 GAAGAGC
ScaI 772 AGTACT AGT^ACT
4327 AGTACT
Tth111I 528 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1393 GACAGCGTC
XbaI 2258 TCTAGA T^CTAGA
2331 TCTAGA
XmnI 262 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3011 GAATAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI BalI
BanII BbsI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HgaI HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII