##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AscI Asp718I AviII BamHI BbrPI
BfrI BglI BsaWI BseAI BsiWI
BspDI BspEI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I ClaI
DraIII DrdI EcoO65I EcoRI FseI
FspI KpnI MfeI MluI MroI
MunI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1572 GACGTC GACGT^C
AccI 2961 GTCTAC GT^[AC][GT]AC
Alw44I 4395 GTGCAC G^TGCAC
AlwNI 21 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1615 CCTCAGG CC^T[ACGT]AGG
Aor51HI 384 AGCGCT AGC^GCT
ApaI 1561 GGGCCC GGGCC^C
ApaLI 4395 GTGCAC G^TGCAC
AspI 1922 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4089 CCTAGG C^CTAGG
BbeI 101 GGCGCC GGCGC^C
BglII 1754 AGATCT A^GATCT
BlnI 4089 CCTAGG C^CTAGG
BsaAI 1337 TACGTA [CT]AC^GT[GA]
BsaI 1970 GAGACC GGTCTC(1/5)
BsgI 4989 GTGCAG GTGCAG(16/14)
BspHI 4132 TCATGA T^CATGA
Bsu36I 1615 CCTCAGG CC^T[ACGT]AGG
CpoI 212 CGGACCG CG^G[AT]CCG
EagI 289 CGGCCG C^GGCCG
EclXI 289 CGGCCG C^GGCCG
Eco47III 384 AGCGCT AGC^GCT
Eco52I 289 CGGCCG C^GGCCG
Eco81I 1615 CCTCAGG CC^T[ACGT]AGG
EcoNI 3662 CCTTGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 3579 AAGCTT A^AGCTT
HpaI 4204 GTTAAC GTT^AAC
KasI 101 GGCGCC G^GCGCC
KspI 480 CCGCGG CCGC^GG
NaeI 2176 GCCGGC GCC^GGC
NarI 101 GGCGCC GG^CGCC
NgoMIV 2176 GCCGGC G^CCGGC
NheI 2085 GCTAGC G^CTAGC
NotI 288 GCGGCCGC GC^GGCCGC
PaeR7I 1018 CTCGAG C^TCGAG
PflFI 1922 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 139 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 4132 TCATGA T^CATGA
RsrII 212 CGGACCG CG^G[AT]CCG
SacI 3146 GAGCTC GAGCT^C
SacII 480 CCGCGG CCGC^GG
SbfI 242 CCTGCAGG CCTGCA^GG
SfoI 101 GGCGCC GGC^GCC
SnaBI 1337 TACGTA TAC^GTA
SpeI 559 ACTAGT A^CTAGT
Sse8387I 242 CCTGCAGG CCTGCA^GG
SspI 3067 AATATT AAT^ATT
Tth111I 1922 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3477 TCTAGA T^CTAGA
XhoI 1018 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 3354 ACATGT A^C[GA][CT]GT
4717 ACATGT
AhdI 3263 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4506 GACAGTCAGTC
AseI 3113 ATTAAT AT^TAAT
5454 ATTAAT
AsnI 3113 ATTAAT AT^TAAT
5454 ATTAAT
AspEI 3263 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4506 GACAGTCAGTC
AspHI 3146 GAGCTC G[AT]GC[AT]^C
4395 GTGCAC
BalI 2204 TGGCCA TGG^CCA
5543 TGGCCA
BanI 101 GGCGCC G^G[CT][GA]CC
2680 GGTGCC
BbvCI 281 CCTCAGC CCTCAGC(-5/-2)
2028 CCTCAGC
BsaBI 4807 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5487 GATTTCTATC
BsiHKAI 3146 GAGCTC G[AT]GC[AT]^C
4395 GTGCAC
BsmBI 391 CGTCTC CGTCTC(1/5)
256 GAGACG
BsmI 5249 GCATTC GAATGC(1/-1)
5470 GCATTC
Bsp1407I 2716 TGTACA T^GTACA
3498 TGTACA
BspLU11I 3354 ACATGT A^CATGT
4717 ACATGT
BsrBI 292 CCGCTC CCGCTC(-3/-3)
29 GAGCGG
BsrFI 1142 ACCGGC [GA]^CCGG[CT]
2176 GCCGGC
BsrGI 2716 TGTACA T^GTACA
3498 TGTACA
BssSI 1596 CTCGTG CACGAG(-5/-1)
1983 CTCGTG
Cfr10I 1142 ACCGGC [GA]^CCGG[CT]
2176 GCCGGC
Eam1105I 3263 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4506 GACAGTCAGTC
MamI 4807 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5487 GATTTCTATC
MluNI 2204 TGGCCA TGG^CCA
5543 TGGCCA
MscI 2204 TGGCCA TGG^CCA
5543 TGGCCA
NcoI 1939 CCATGG C^CATGG
4097 CCATGG
PflMI 4022 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5022 CCAGCATATGG
PshBI 3113 ATTAAT AT^TAAT
5454 ATTAAT
PvuI 86 CGATCG CGAT^CG
4998 CGATCG
SmaI 157 CCCGGG CCC^GGG
5094 CCCGGG
SspBI 2716 TGTACA T^GTACA
3498 TGTACA
Van91I 4022 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5022 CCAGCATATGG
XmaCI 157 CCCGGG C^CCGGG
5094 CCCGGG
XmaI 157 CCCGGG C^CCGGG
5094 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI Asp700I AvaI
AvaII BanII BbsI BbvI BcgI
BciVI BclI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BseRI BsiEI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoRV EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeI NdeII NlaIII
NlaIV NsiI NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI