##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AccIII AgeI AhdI Aor51HI
AscI AspEI AviII AvrII BglII
BlnI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BssHII Bst1107I BstZ17I
ClaI EagI Eam1105I EclXI Eco47III
Eco52I EcoT22I FseI FspI HpaI
KspI MfeI MluI MroI MunI
NdeI NotI NruI NsiI PacI
PinAI PmeI PvuI SacII SapI
SbfI SfiI SgrAI SnaBI Sse8387I
SspI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 2116 GGTACC G^GTACC
AclI 1176 AACGTT AA^CGTT
AflII 2222 CTTAAG C^TTAAG
AflIII 3510 ACACGT A^C[GA][CT]GT
Alw44I 649 GTGCAC G^TGCAC
AocI 4012 CCTCAGG CC^T[ACGT]AGG
ApaI 3497 GGGCCC GGGCC^C
ApaLI 649 GTGCAC G^TGCAC
Asp718I 2116 GGTACC G^GTACC
AspI 4937 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1911 GGATCC G^GATCC
BbeI 369 GGCGCC GGCGC^C
BbrPI 3511 CACGTG CAC^GTG
BfrI 2222 CTTAAG C^TTAAG
BglI 372 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsrBI 2999 GAGCGG CCGCTC(-3/-3)
BssSI 2597 CACGAG CACGAG(-5/-1)
BstBI 3678 TTCGAA TT^CGAA
Bsu36I 4012 CCTCAGG CC^T[ACGT]AGG
CpoI 3840 CGGTCCG CG^G[AT]CCG
DraIII 2150 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 4012 CCTCAGG CC^T[ACGT]AGG
HindIII 4741 AAGCTT A^AGCTT
KasI 369 GGCGCC G^GCGCC
KpnI 2116 GGTACC GGTAC^C
NarI 369 GGCGCC GG^CGCC
NheI 967 GCTAGC G^CTAGC
NspV 3678 TTCGAA TT^CGAA
PaeR7I 3174 CTCGAG C^TCGAG
PflFI 4937 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3511 CACGTG CAC^GTG
PmlI 3511 CACGTG CAC^GTG
PshAI 3030 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1176 AACGTT AA^CGTT
RsrII 3840 CGGTCCG CG^G[AT]CCG
SacI 1355 GAGCTC GAGCT^C
SalI 1339 GTCGAC G^TCGAC
ScaI 4350 AGTACT AGT^ACT
SfoI 369 GGCGCC GGC^GCC
SfuI 3678 TTCGAA TT^CGAA
SmaI 3213 CCCGGG CCC^GGG
SpeI 2987 ACTAGT A^CTAGT
SphI 3861 GCATGC GCATG^C
Tth111I 4937 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3094 TCTAGA T^CTAGA
XhoI 3174 CTCGAG C^TCGAG
XmaCI 3213 CCCGGG C^CCGGG
XmaI 3213 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AseI 3434 ATTAAT AT^TAAT
5305 ATTAAT
AsnI 3434 ATTAAT AT^TAAT
5305 ATTAAT
BcgI 1845 CGATCATCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
636 GCAATGTTATCG
BclI 3084 TGATCA T^GATCA
5082 TGATCA
BsaAI 3511 CACGTG [CT]AC^GT[GA]
5614 TACGTG
BsaWI 2008 ACCGGA [AT]^CCGG[AT]
2389 TCCGGT
BsmBI 5884 CGTCTC CGTCTC(1/5)
719 GAGACG
BsmI 680 GAATGC GAATGC(1/-1)
5719 GAATGC
BspHI 4032 TCATGA T^CATGA
4645 TCATGA
BstEII 2338 GGTGACC G^GT[ACGT]ACC
3664 GGTCACC
BstPI 2338 GGTGACC G^GT[ACGT]ACC
3664 GGTCACC
BstXI 1344 CCATTCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4148 CCATCGCGCTGG
DraI 1681 TTTAAA TTT^AAA
5098 TTTAAA
DrdI 2515 GACGCTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5320 GACCCCATAGTC
EcoO65I 2338 GGTGACC G^GT[ACGT]ACC
3664 GGTCACC
EcoRI 4578 GAATTC G^AATTC
5983 GAATTC
FbaI 3084 TGATCA T^GATCA
5082 TGATCA
NaeI 971 GCCGGC GCC^GGC
2935 GCCGGC
NgoMIV 971 GCCGGC G^CCGGC
2935 GCCGGC
PshBI 3434 ATTAAT AT^TAAT
5305 ATTAAT
RcaI 4032 TCATGA T^CATGA
4645 TCATGA
SexAI 954 ACCAGGT A^CC[AT]GGT
2334 ACCTGGT
StuI 1761 AGGCCT AGG^CCT
3115 AGGCCT
SwaI 1680 ATTTAAAT ATTT^AAAT
5097 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrDI BsrFI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI