##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
Asp718I AvrII BamHI BclI BfrI
BglII BlnI BsaBI BsiWI BsmI
BspDI BspHI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI DraI EcoO65I EcoT22I FbaI
HpaI KpnI MamI MfeI MunI
NdeI NheI NsiI NspV PacI
PaeR7I PinAI PmeI Psp1406I PvuI
RcaI SalI SfiI SfuI SgrAI
SmlI SnaBI SphI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 2950 GACGTC GACGT^C
AocI 4139 CCTGAGG CC^T[ACGT]AGG
AseI 2422 ATTAAT AT^TAAT
AsnI 2422 ATTAAT AT^TAAT
AviII 1488 TGCGCA TGC^GCA
BbrPI 4913 CACGTG CAC^GTG
BbvCI 3480 CCTCAGC CCTCAGC(-5/-2)
BcgI 3972 GCATCCCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2943 GGATAC GTATCC(6/5)
BsaAI 4913 CACGTG [CT]AC^GT[GA]
BsmBI 3991 CGTCTC CGTCTC(1/5)
BspLU11I 930 ACATGT A^CATGT
BstXI 4785 CCAAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4139 CCTGAGG CC^T[ACGT]AGG
CpoI 728 CGGACCG CG^G[AT]CCG
EagI 383 CGGCCG C^GGCCG
EclXI 383 CGGCCG C^GGCCG
Eco52I 383 CGGCCG C^GGCCG
Eco81I 4139 CCTGAGG CC^T[ACGT]AGG
EcoRI 629 GAATTC G^AATTC
EcoRV 5105 GATATC GAT^ATC
FseI 4007 GGCCGGCC GGCCGG^CC
FspI 1488 TGCGCA TGC^GCA
HincII 1267 GTTGAC GT[CT]^[GA]AC
HindII 1267 GTTGAC GT[CT]^[GA]AC
MluI 1856 ACGCGT A^CGCGT
NotI 382 GCGGCCGC GC^GGCCGC
NruI 1923 TCGCGA TCG^CGA
PmaCI 4913 CACGTG CAC^GTG
PmlI 4913 CACGTG CAC^GTG
PshBI 2422 ATTAAT AT^TAAT
RsrII 728 CGGACCG CG^G[AT]CCG
ScaI 2915 AGTACT AGT^ACT
SpeI 2918 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 2471 GTCTAC GT^[AC][GT]AC
4850 GTCTAC
AhdI 1505 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4835 GACTTTGTGTC
Alw44I 5207 GTGCAC G^TGCAC
5404 GTGCAC
Aor51HI 3690 AGCGCT AGC^GCT
3871 AGCGCT
ApaLI 5207 GTGCAC G^TGCAC
5404 GTGCAC
AspEI 1505 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4835 GACTTTGTGTC
Bsp1407I 1830 TGTACA T^GTACA
3720 TGTACA
BsrGI 1830 TGTACA T^GTACA
3720 TGTACA
BstAPI 400 GCACGCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4078 GCAGACTCTGC
DraIII 332 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
401 CACGCGGTG
Eam1105I 1505 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4835 GACTTTGTGTC
Eco47III 3690 AGCGCT AGC^GCT
3871 AGCGCT
HindIII 3167 AAGCTT A^AGCTT
4877 AAGCTT
KspI 228 CCGCGG CCGC^GG
2688 CCGCGG
PshAI 1249 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1789 GACAGCCGTC
SacII 228 CCGCGG CCGC^GG
2688 CCGCGG
SexAI 1611 ACCAGGT A^CC[AT]GGT
4947 ACCTGGT
SspBI 1830 TGTACA T^GTACA
3720 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI Asp700I AspHI AspI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SfoI
SmaI Sse8387I StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI