##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AviII AvrII BanI BbeI
BbrPI BbvCI BglI BlnI BlpI
Bpu1102I BsaHI BsaI BseAI BseRI
BsiEI BsiWI BspEI BspMI BsrBI
BssHII BssSI BstBI BstUI Bsu36I
CelII CfoI CpoI DraII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV FseI FspI HaeII HhaI
Hin1I HinP1I HindIII KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PvuI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I StuI Van91I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 3368 GTATAC GT^[AC][GT]AC
AclI 3902 AACGTT AA^CGTT
AspHI 879 GAGCAC G[AT]GC[AT]^C
AvaI 10 CTCGGG C^[CT]CG[GA]G
BalI 2569 TGGCCA TGG^CCA
BamHI 555 GGATCC G^GATCC
BanII 5093 GAGCCC G[GA]GC[CT]^C
BcgI 824 CGATGAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 805 CCCGG CC^[GC]GG
BsaAI 3669 TACGTG [CT]AC^GT[GA]
BsaWI 4898 ACCGGA [AT]^CCGG[AT]
BsiHKAI 879 GAGCAC G[AT]GC[AT]^C
BsmBI 2813 GAGACG CGTCTC(1/5)
BsoBI 10 CTCGGG C^[CT]CG[GA]G
BspDI 396 ATCGAT AT^CGAT
BspLU11I 772 ACATGT A^CATGT
BsrDI 594 GCAATG GCAATG(2/0)
BsrFI 69 GCCGGT [GA]^CCGG[CT]
Bst1107I 3368 GTATAC GTA^TAC
BstAPI 4642 GCATTAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4377 GGTGACC G^GT[ACGT]ACC
BstPI 4377 GGTGACC G^GT[ACGT]ACC
BstZ17I 3368 GTATAC GTA^TAC
Cfr10I 69 GCCGGT [GA]^CCGG[CT]
ClaI 396 ATCGAT AT^CGAT
DraIII 960 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2569 TGGCCA [CT]^GGCC[GA]
EcoO65I 4377 GGTGACC G^GT[ACGT]ACC
EcoT14I 1121 CCAAGG C^C[AT][AT]GG
EcoT22I 2723 ATGCAT ATGCA^T
FauI 35 CCCGC CCCGC(4/6)
HincII 3550 GTTAAC GT[CT]^[GA]AC
HindII 3550 GTTAAC GT[CT]^[GA]AC
HpaI 3550 GTTAAC GTT^AAC
MluNI 2569 TGGCCA TGG^CCA
MscI 2569 TGGCCA TGG^CCA
NciI 805 CCCGG CC^[GC]GG
NsiI 2723 ATGCAT ATGCA^T
Psp1406I 3902 AACGTT AA^CGTT
PstI 439 CTGCAG CTGCA^G
PvuII 4321 CAGCTG CAG^CTG
StyI 1121 CCAAGG C^C[AT][AT]GG
SwaI 2350 ATTTAAAT ATTT^AAAT
XbaI 5262 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 772 ACATGT A^C[GA][CT]GT
1893 ACACGT
AseI 2793 ATTAAT AT^TAAT
4054 ATTAAT
AsnI 2793 ATTAAT AT^TAAT
4054 ATTAAT
AspI 1852 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1916 GACTGTGTC
AvaII 522 GGTCC G^G[AT]CC
1857 GGTCC
BciVI 599 GGATAC GTATCC(6/5)
3240 GGATAC
BclI 1684 TGATCA T^GATCA
2177 TGATCA
BsaBI 399 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5131 GATCAATATC
BsgI 3157 CTGCAC GTGCAG(16/14)
5269 CTGCAC
BsiYI 103 CCGTAATGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
5096 CCCCTGGCTGG
BslI 103 CCGTAATGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
5096 CCCCTGGCTGG
BsmI 434 GAATGC GAATGC(1/-1)
4423 GAATGC
Cac8I 2140 GCAAGC GC[ACGT]^[ACGT]GC
2904 GCAGGC
FbaI 1684 TGATCA T^GATCA
2177 TGATCA
HaeIII 2570 GGCC GG^CC
3292 GGCC
HgaI 47 GCGTC GACGC(5/10)
97 GCGTC
MamI 399 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5131 GATCAATATC
MspA1I 36 CCGCTG C[AC]G^C[GT]G
4321 CAGCTG
NlaIV 555 GGATCC GG[ACGT]^[ACGT]CC
3787 GGCTCC
PflFI 1852 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1916 GACTGTGTC
PshBI 2793 ATTAAT AT^TAAT
4054 ATTAAT
SapI 3113 GCTCTTC GCTCTTC(1/4)
5227 GCTCTTC
ScaI 4497 AGTACT AGT^ACT
4782 AGTACT
SfcI 439 CTGCAG C^T[GA][CT]AG
1442 CTGTAG
Tth111I 1852 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1916 GACTGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI Asp700I BbsI BbvI
BfaI BfrI BglII BmyI BpmI
BpuAI BsaJI BsmAI BsmFI Bsp1286I
Bsp1407I BspHI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cfr13I
DdeI DpnII DraI EarI EcoRII
Fnu4HI FokI HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeI NdeII NlaIII NspI PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI SpeI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI