##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AccI AclI AflII AscI AviII
BbvCI BcgI BciVI BclI BfrI
BsaBI BsiWI BspDI Bst1107I BstXI
BstZ17I ClaI CpoI EcoRV FbaI
FseI FspI HindIII HpaI MamI
MfeI MluI MunI NcoI NdeI
NruI PacI PmeI PshAI Psp1406I
RsrII SalI ScaI SnaBI SpeI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 4274 GGTACC G^GTACC
AocI 5342 CCTGAGG CC^T[ACGT]AGG
Asp700I 2362 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4274 GGTACC G^GTACC
AvrII 1663 CCTAGG C^CTAGG
BbsI 3794 GAAGAC GAAGAC(2/6)
BlnI 1663 CCTAGG C^CTAGG
BpuAI 3794 GAAGAC GAAGAC(2/6)
Bsp1407I 5253 TGTACA T^GTACA
BspLU11I 2562 ACATGT A^CATGT
BsrGI 5253 TGTACA T^GTACA
BssHII 2 GCGCGC G^CGCGC
Bsu36I 5342 CCTGAGG CC^T[ACGT]AGG
DraI 835 TTTAAA TTT^AAA
DrdI 795 GACTAGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5342 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2650 ATGCAT ATGCA^T
KpnI 4274 GGTACC GGTAC^C
NheI 2527 GCTAGC G^CTAGC
NotI 3158 GCGGCCGC GC^GGCCGC
NsiI 2650 ATGCAT ATGCA^T
PaeR7I 1498 CTCGAG C^TCGAG
PvuI 4630 CGATCG CGAT^CG
SbfI 3006 CCTGCAGG CCTGCA^GG
SfiI 5015 GGCCTGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 3006 CCTGCAGG CCTGCA^GG
SspBI 5253 TGTACA T^GTACA
SspI 2406 AATATT AAT^ATT
XhoI 1498 CTCGAG C^TCGAG
XmnI 2362 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 2537 GACGTC GACGT^C
4825 GACGTC
AccIII 1591 TCCGGA T^CCGGA
3125 TCCGGA
AgeI 3288 ACCGGT A^CCGGT
5363 ACCGGT
AhdI 3116 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3965 GACAGCCCGTC
Aor51HI 1764 AGCGCT AGC^GCT
4834 AGCGCT
AseI 406 ATTAAT AT^TAAT
3704 ATTAAT
AsnI 406 ATTAAT AT^TAAT
3704 ATTAAT
AspEI 3116 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3965 GACAGCCCGTC
BamHI 3472 GGATCC G^GATCC
3640 GGATCC
BsaI 342 GAGACC GGTCTC(1/5)
4378 GGTCTC
BseAI 1591 TCCGGA T^CCGGA
3125 TCCGGA
BsmBI 4075 CGTCTC CGTCTC(1/5)
128 GAGACG
BspEI 1591 TCCGGA T^CCGGA
3125 TCCGGA
BstBI 464 TTCGAA TT^CGAA
2016 TTCGAA
BstEII 973 GGTGACC G^GT[ACGT]ACC
1102 GGTTACC
BstPI 973 GGTGACC G^GT[ACGT]ACC
1102 GGTTACC
DraIII 279 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
434 CACATGGTG
Eam1105I 3116 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3965 GACAGCCCGTC
Eco47III 1764 AGCGCT AGC^GCT
4834 AGCGCT
EcoO65I 973 GGTGACC G^GT[ACGT]ACC
1102 GGTTACC
EcoRI 1280 GAATTC G^AATTC
2366 GAATTC
HincII 2182 GTTGAC GT[CT]^[GA]AC
2504 GTCAAC
HindII 2182 GTTGAC GT[CT]^[GA]AC
2504 GTCAAC
MroI 1591 TCCGGA T^CCGGA
3125 TCCGGA
NaeI 248 GCCGGC GCC^GGC
2262 GCCGGC
NgoMIV 248 GCCGGC G^CCGGC
2262 GCCGGC
NspV 464 TTCGAA TT^CGAA
2016 TTCGAA
PinAI 3288 ACCGGT A^CCGGT
5363 ACCGGT
PshBI 406 ATTAAT AT^TAAT
3704 ATTAAT
SfuI 464 TTCGAA TT^CGAA
2016 TTCGAA
SgrAI 4777 CACCGGCG C[GA]^CCGG[CT]G
5362 CACCGGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 155 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspHI AspI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbvI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RcaI
RsaI SacI SacII SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI