##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AclI AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp700I
Asp718I AspEI AviII BamHI BbrPI
BbsI BcgI BglII BpuAI BsaAI
BsiWI BspDI BspLU11I BsrBI BsrFI
Bst1107I BstBI BstZ17I Cfr10I ClaI
Eam1105I Eco47III EcoRI FseI FspI
HindIII KpnI MfeI MluI MunI
NaeI NdeI NgoMIV NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
Sse8387I SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 3231 GACGTC GACGT^C
AflII 3772 CTTAAG C^TTAAG
AocI 1845 CCTCAGG CC^T[ACGT]AGG
AseI 4643 ATTAAT AT^TAAT
AsnI 4643 ATTAAT AT^TAAT
AvrII 568 CCTAGG C^CTAGG
BfrI 3772 CTTAAG C^TTAAG
BlnI 568 CCTAGG C^CTAGG
BlpI 4443 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4443 GCTAAGC GC^T[ACGT]AGC
BsiEI 26 CGGCCG CG[GA][CT]^CG
Bsp1407I 3052 TGTACA T^GTACA
BspHI 3710 TCATGA T^CATGA
BsrGI 3052 TGTACA T^GTACA
BssHII 211 GCGCGC G^CGCGC
BssSI 4079 CTCGTG CACGAG(-5/-1)
BstEII 57 GGTCACC G^GT[ACGT]ACC
BstPI 57 GGTCACC G^GT[ACGT]ACC
Bsu36I 1845 CCTCAGG CC^T[ACGT]AGG
CelII 4443 GCTAAGC GC^T[ACGT]AGC
CpoI 2 CGGTCCG CG^G[AT]CCG
DraI 4578 TTTAAA TTT^AAA
DrdI 1498 GACTCAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 26 CGGCCG C^GGCCG
EclXI 26 CGGCCG C^GGCCG
Eco52I 26 CGGCCG C^GGCCG
Eco81I 1845 CCTCAGG CC^T[ACGT]AGG
EcoO65I 57 GGTCACC G^GT[ACGT]ACC
HgaI 84 GACGC GACGC(5/10)
HpaI 2538 GTTAAC GTT^AAC
NcoI 1164 CCATGG C^CATGG
NheI 491 GCTAGC G^CTAGC
NotI 25 GCGGCCGC GC^GGCCGC
PflMI 742 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 335 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4643 ATTAAT AT^TAAT
RcaI 3710 TCATGA T^CATGA
RsrII 2 CGGTCCG CG^G[AT]CCG
ScaI 3679 AGTACT AGT^ACT
SpeI 1316 ACTAGT A^CTAGT
SspBI 3052 TGTACA T^GTACA
Van91I 742 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2105 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 227 TCCGGA T^CCGGA
3274 TCCGGA
ApaI 38 GGGCCC GGGCC^C
3108 GGGCCC
AspI 1101 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2886 GACTCCGTC
BbeI 985 GGCGCC GGCGC^C
2767 GGCGCC
BclI 1795 TGATCA T^GATCA
3414 TGATCA
BsaBI 3578 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4062 GATCCCAATC
BseAI 227 TCCGGA T^CCGGA
3274 TCCGGA
BspEI 227 TCCGGA T^CCGGA
3274 TCCGGA
BsrDI 1288 CATTGC GCAATG(2/0)
574 GCAATG
BstAPI 2208 GCACTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2827 GCAGAGGCTGC
DraIII 641 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
806 CACAATGTG
EcoRV 2352 GATATC GAT^ATC
2932 GATATC
FbaI 1795 TGATCA T^GATCA
3414 TGATCA
HincII 616 GTCAAC GT[CT]^[GA]AC
2538 GTTAAC
HindII 616 GTCAAC GT[CT]^[GA]AC
2538 GTTAAC
KasI 985 GGCGCC G^GCGCC
2767 GGCGCC
KspI 29 CCGCGG CCGC^GG
43 CCGCGG
MaeII 3093 ACGT A^CGT
3232 ACGT
MamI 3578 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4062 GATCCCAATC
MroI 227 TCCGGA T^CCGGA
3274 TCCGGA
NarI 985 GGCGCC GG^CGCC
2767 GGCGCC
PflFI 1101 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2886 GACTCCGTC
SacI 1960 GAGCTC GAGCT^C
3500 GAGCTC
SacII 29 CCGCGG CCGC^GG
43 CCGCGG
SfoI 985 GGCGCC GGC^GCC
2767 GGCGCC
SphI 1875 GCATGC GCATG^C
3992 GCATGC
Tth111I 1101 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2886 GACTCCGTC
XcmI 291 CCAGAATGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
665 CCAACCCTCTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BciVI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI