################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## Acc65I AclI AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp700I Asp718I AspEI AviII BamHI BbrPI BbsI BcgI BglII BpuAI BsaAI BsiWI BspDI BspLU11I BsrBI BsrFI Bst1107I BstBI BstZ17I Cfr10I ClaI Eam1105I Eco47III EcoRI FseI FspI HindIII KpnI MfeI MluI MunI NaeI NdeI NgoMIV NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI SalI SbfI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 3231 GACGTC GACGT^C AflII 3772 CTTAAG C^TTAAG AocI 1845 CCTCAGG CC^T[ACGT]AGG AseI 4643 ATTAAT AT^TAAT AsnI 4643 ATTAAT AT^TAAT AvrII 568 CCTAGG C^CTAGG BfrI 3772 CTTAAG C^TTAAG BlnI 568 CCTAGG C^CTAGG BlpI 4443 GCTAAGC GC^T[ACGT]AGC Bpu1102I 4443 GCTAAGC GC^T[ACGT]AGC BsiEI 26 CGGCCG CG[GA][CT]^CG Bsp1407I 3052 TGTACA T^GTACA BspHI 3710 TCATGA T^CATGA BsrGI 3052 TGTACA T^GTACA BssHII 211 GCGCGC G^CGCGC BssSI 4079 CTCGTG CACGAG(-5/-1) BstEII 57 GGTCACC G^GT[ACGT]ACC BstPI 57 GGTCACC G^GT[ACGT]ACC Bsu36I 1845 CCTCAGG CC^T[ACGT]AGG CelII 4443 GCTAAGC GC^T[ACGT]AGC CpoI 2 CGGTCCG CG^G[AT]CCG DraI 4578 TTTAAA TTT^AAA DrdI 1498 GACTCAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 26 CGGCCG C^GGCCG EclXI 26 CGGCCG C^GGCCG Eco52I 26 CGGCCG C^GGCCG Eco81I 1845 CCTCAGG CC^T[ACGT]AGG EcoO65I 57 GGTCACC G^GT[ACGT]ACC HgaI 84 GACGC GACGC(5/10) HpaI 2538 GTTAAC GTT^AAC NcoI 1164 CCATGG C^CATGG NheI 491 GCTAGC G^CTAGC NotI 25 GCGGCCGC GC^GGCCGC PflMI 742 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 335 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4643 ATTAAT AT^TAAT RcaI 3710 TCATGA T^CATGA RsrII 2 CGGTCCG CG^G[AT]CCG ScaI 3679 AGTACT AGT^ACT SpeI 1316 ACTAGT A^CTAGT SspBI 3052 TGTACA T^GTACA Van91I 742 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2105 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccIII 227 TCCGGA T^CCGGA 3274 TCCGGA ApaI 38 GGGCCC GGGCC^C 3108 GGGCCC AspI 1101 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2886 GACTCCGTC BbeI 985 GGCGCC GGCGC^C 2767 GGCGCC BclI 1795 TGATCA T^GATCA 3414 TGATCA BsaBI 3578 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4062 GATCCCAATC BseAI 227 TCCGGA T^CCGGA 3274 TCCGGA BspEI 227 TCCGGA T^CCGGA 3274 TCCGGA BsrDI 1288 CATTGC GCAATG(2/0) 574 GCAATG BstAPI 2208 GCACTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2827 GCAGAGGCTGC DraIII 641 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 806 CACAATGTG EcoRV 2352 GATATC GAT^ATC 2932 GATATC FbaI 1795 TGATCA T^GATCA 3414 TGATCA HincII 616 GTCAAC GT[CT]^[GA]AC 2538 GTTAAC HindII 616 GTCAAC GT[CT]^[GA]AC 2538 GTTAAC KasI 985 GGCGCC G^GCGCC 2767 GGCGCC KspI 29 CCGCGG CCGC^GG 43 CCGCGG MaeII 3093 ACGT A^CGT 3232 ACGT MamI 3578 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4062 GATCCCAATC MroI 227 TCCGGA T^CCGGA 3274 TCCGGA NarI 985 GGCGCC GG^CGCC 2767 GGCGCC PflFI 1101 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2886 GACTCCGTC SacI 1960 GAGCTC GAGCT^C 3500 GAGCTC SacII 29 CCGCGG CCGC^GG 43 CCGCGG SfoI 985 GGCGCC GGC^GCC 2767 GGCGCC SphI 1875 GCATGC GCATG^C 3992 GCATGC Tth111I 1101 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2886 GACTCCGTC XcmI 291 CCAGAATGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 665 CCAACCCTCTCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BciVI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI