##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI Alw44I ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BcgI BfrI BlnI
BsaAI BseAI BsiWI BspEI BspHI
BspMI BsrDI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstZ17I CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV FseI FspI HindIII
HpaI KpnI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SalI SbfI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflIII 593 ACATGT A^C[GA][CT]GT
Aor51HI 13 AGCGCT AGC^GCT
BalI 910 TGGCCA TGG^CCA
BbvCI 289 GCTGAGG CCTCAGC(-5/-2)
BclI 2240 TGATCA T^GATCA
BlpI 4137 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4137 GCTCAGC GC^T[ACGT]AGC
BsaBI 2157 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1800 CGGTCG CG[GA][CT]^CG
BsmI 1493 GCATTC GAATGC(1/-1)
Bsp1407I 4432 TGTACA T^GTACA
BspDI 3853 ATCGAT AT^CGAT
BspLU11I 593 ACATGT A^CATGT
BsrGI 4432 TGTACA T^GTACA
BstBI 533 TTCGAA TT^CGAA
BstXI 413 CCAGTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 4137 GCTCAGC GC^T[ACGT]AGC
ClaI 3853 ATCGAT AT^CGAT
Eco47III 13 AGCGCT AGC^GCT
EcoNI 3934 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2041 ATGCAT ATGCA^T
FbaI 2240 TGATCA T^GATCA
HincII 3400 GTCAAC GT[CT]^[GA]AC
HindII 3400 GTCAAC GT[CT]^[GA]AC
KspI 181 CCGCGG CCGC^GG
MamI 2157 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 910 TGGCCA TGG^CCA
MscI 910 TGGCCA TGG^CCA
NcoI 2184 CCATGG C^CATGG
NsiI 2041 ATGCAT ATGCA^T
NspV 533 TTCGAA TT^CGAA
PpuMI 3611 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 2050 GAGCTC GAGCT^C
SacII 181 CCGCGG CCGC^GG
SexAI 2537 ACCTGGT A^CC[AT]GGT
SfuI 533 TTCGAA TT^CGAA
SmaI 2002 CCCGGG CCC^GGG
SspBI 4432 TGTACA T^GTACA
XbaI 1734 TCTAGA T^CTAGA
XmaCI 2002 CCCGGG C^CCGGG
XmaI 2002 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 3441 GTAGAC GT^[AC][GT]AC
3553 GTAGAC
AcyI 177 GGCGCC G[GA]^CG[CT]C
2405 GGCGCC
AgeI 3517 ACCGGT A^CCGGT
3791 ACCGGT
AocI 1458 CCTCAGG CC^T[ACGT]AGG
4104 CCTCAGG
AseI 1085 ATTAAT AT^TAAT
1986 ATTAAT
AsnI 1085 ATTAAT AT^TAAT
1986 ATTAAT
Asp700I 482 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1672 GAACACATTC
AspHI 2050 GAGCTC G[AT]GC[AT]^C
4748 GAGCAC
BbeI 177 GGCGCC GGCGC^C
2405 GGCGCC
BciVI 3469 GGATAC GTATCC(6/5)
3205 GTATCC
BglII 1048 AGATCT A^GATCT
1872 AGATCT
BsaHI 177 GGCGCC G[GA]^CG[CT]C
2405 GGCGCC
BsaI 3715 GAGACC GGTCTC(1/5)
4797 GGTCTC
BsgI 416 GTGCAG GTGCAG(16/14)
2616 GTGCAG
BsiHKAI 2050 GAGCTC G[AT]GC[AT]^C
4748 GAGCAC
BsmBI 396 CGTCTC CGTCTC(1/5)
2298 GAGACG
Bsu36I 1458 CCTCAGG CC^T[ACGT]AGG
4104 CCTCAGG
DraI 2117 TTTAAA TTT^AAA
4765 TTTAAA
Eco81I 1458 CCTCAGG CC^T[ACGT]AGG
4104 CCTCAGG
HgaI 395 GCGTC GACGC(5/10)
1500 GCGTC
Hin1I 177 GGCGCC G[GA]^CG[CT]C
2405 GGCGCC
KasI 177 GGCGCC G^GCGCC
2405 GGCGCC
NarI 177 GGCGCC GG^CGCC
2405 GGCGCC
NspI 593 ACATGT [GA]CATG^[CT]
4133 ACATGC
PinAI 3517 ACCGGT A^CCGGT
3791 ACCGGT
PshAI 2342 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3243 GACATTCGTC
PshBI 1085 ATTAAT AT^TAAT
1986 ATTAAT
SapI 2052 GCTCTTC GCTCTTC(1/4)
3351 GCTCTTC
ScaI 806 AGTACT AGT^ACT
1896 AGTACT
SfoI 177 GGCGCC GGC^GCC
2405 GGCGCC
XcmI 2798 CCAGATTTCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3735 CCAGAGAAGCCCTGG
XmnI 482 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1672 GAACACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII