##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Alw44I Aor51HI ApaLI AscI
AspI AviII BbeI BbrPI BcgI
BglI BglII BsaAI BsaBI BsaHI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HgaI Hin1I KasI KspI
MamI MluI MroI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspBI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 4580 GGTACC G^GTACC
AccI 3508 GTAGAC GT^[AC][GT]AC
AhdI 4343 GACGTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 52 GGGCCC GGGCC^C
Asp718I 4580 GGTACC G^GTACC
AspEI 4343 GACGTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2057 GAGCTC G[AT]GC[AT]^C
AvrII 2476 CCTAGG C^CTAGG
BamHI 3767 GGATCC G^GATCC
BclI 211 TGATCA T^GATCA
BlnI 2476 CCTAGG C^CTAGG
BlpI 3595 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3595 GCTCAGC GC^T[ACGT]AGC
BsaI 1229 GGTCTC GGTCTC(1/5)
BseRI 2413 CTCCTC GAGGAG(10/8)
BsiHKAI 2057 GAGCTC G[AT]GC[AT]^C
BspLU11I 4735 ACATGT A^CATGT
BsrBI 777 GAGCGG CCGCTC(-3/-3)
BstXI 1692 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3595 GCTCAGC GC^T[ACGT]AGC
CfoI 770 GCGC GCG^C
DsaI 1809 CCGTGG C^C[GA][CT]GG
Eam1105I 4343 GACGTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 779 GCGGG CCCGC(4/6)
FbaI 211 TGATCA T^GATCA
HaeII 769 GGCGCT [GA]GCGC^[CT]
HhaI 770 GCGC GCG^C
HinP1I 770 GCGC G^CGC
KpnI 4580 GGTACC GGTAC^C
MfeI 5705 CAATTG C^AATTG
MunI 5705 CAATTG C^AATTG
NdeI 630 CATATG CA^TATG
SacI 2057 GAGCTC GAGCT^C
SexAI 1258 ACCTGGT A^CC[AT]GGT
SmaI 1526 CCCGGG CCC^GGG
SpeI 4387 ACTAGT A^CTAGT
StuI 1269 AGGCCT AGG^CCT
XmaCI 1526 CCCGGG C^CCGGG
XmaI 1526 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 2465 CTTAAG C^TTAAG
2972 CTTAAG
AflIII 172 ACACGT A^C[GA][CT]GT
4735 ACATGT
BanI 782 GGCACC G^G[CT][GA]CC
4580 GGTACC
BciVI 3828 GGATAC GTATCC(6/5)
4146 GTATCC
BfrI 2465 CTTAAG C^TTAAG
2972 CTTAAG
BsaWI 3415 TCCGGT [AT]^CCGG[AT]
3948 ACCGGA
BsmBI 687 GAGACG CGTCTC(1/5)
1620 GAGACG
BspHI 1403 TCATGA T^CATGA
2903 TCATGA
BstYI 2093 GGATCT [GA]^GATC[CT]
3767 GGATCC
EcoNI 3446 CCTACATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4260 CCTCCCCCAGG
HpaI 2503 GTTAAC GTT^AAC
4466 GTTAAC
MflI 2093 GGATCT [GA]^GATC[CT]
3767 GGATCC
MspA1I 657 CAGCTG C[AC]G^C[GT]G
5397 CAGCTG
NspI 1579 GCATGT [GA]CATG^[CT]
4735 ACATGT
PflMI 3134 CCATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3563 CCACATCCTGG
PvuII 657 CAGCTG CAG^CTG
5397 CAGCTG
RcaI 1403 TCATGA T^CATGA
2903 TCATGA
TaqI 1222 TCGA T^CGA
3721 TCGA
TthHB8I 1222 TCGA T^CGA
3721 TCGA
Van91I 3134 CCATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3563 CCACATCCTGG
XcmI 451 CCAAGAAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
649 CCAACAAGCAGCTGG
XhoII 2093 GGATCT [GA]^GATC[CT]
3767 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
Asp700I AvaI AvaII BalI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BssSI BstAPI BstF5I BstNI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PshBI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XbaI XmnI