################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## Acc65I AccII AflII AflIII AgeI Aor51HI AscI Asp718I AviII AvrII BanI BbeI BciVI BfrI BlnI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII BssSI BstEII BstPI BstUI CfoI ClaI CpoI DrdI Eco47III EcoO65I EcoRI FspI HaeII HhaI HinP1I HpaI KasI KpnI KspI MluI MvnI NarI NheI NotI NruI PacI PaeR7I PinAI PmeI PshAI PvuI RcaI RsrII SacII SalI SapI SbfI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SspBI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 3447 GACGTC GACGT^C AclI 502 AACGTT AA^CGTT AcyI 3447 GACGTC G[GA]^CG[CT]C AhdI 3442 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3453 GTGCAC G^TGCAC AocI 2015 CCTGAGG CC^T[ACGT]AGG ApaLI 3453 GTGCAC G^TGCAC AspEI 3442 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 2789 GGATCC G^GATCC BbrPI 3419 CACGTG CAC^GTG BcgI 699 GCACTTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 3776 TGATCA T^GATCA BcnI 3643 CCCGG CC^[GC]GG BglI 2250 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1362 AGATCT A^GATCT BlpI 2651 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2651 GCTCAGC GC^T[ACGT]AGC BsaBI 375 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 3447 GACGTC G[GA]^CG[CT]C BsrBI 4852 GAGCGG CCGCTC(-3/-3) Bst1107I 3016 GTATAC GTA^TAC BstBI 238 TTCGAA TT^CGAA BstZ17I 3016 GTATAC GTA^TAC Bsu36I 2015 CCTGAGG CC^T[ACGT]AGG CelII 2651 GCTCAGC GC^T[ACGT]AGC Eam1105I 3442 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2015 CCTGAGG CC^T[ACGT]AGG FbaI 3776 TGATCA T^GATCA FseI 5112 GGCCGGCC GGCCGG^CC Hin1I 3447 GACGTC G[GA]^CG[CT]C HindIII 4493 AAGCTT A^AGCTT MamI 375 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2045 CAATTG C^AATTG MunI 2045 CAATTG C^AATTG NciI 3643 CCCGG CC^[GC]GG NspV 238 TTCGAA TT^CGAA PmaCI 3419 CACGTG CAC^GTG PmlI 3419 CACGTG CAC^GTG Psp1406I 502 AACGTT AA^CGTT SfuI 238 TTCGAA TT^CGAA SpeI 2515 ACTAGT A^CTAGT SphI 1207 GCATGC GCATG^C ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AccIII 919 TCCGGA T^CCGGA 2659 TCCGGA ApaI 935 GGGCCC GGGCC^C 3640 GGGCCC AseI 2732 ATTAAT AT^TAAT 3220 ATTAAT AsnI 2732 ATTAAT AT^TAAT 3220 ATTAAT Asp700I 130 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1441 GAAACTATTC AspI 3734 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3902 GACGAGGTC AvaI 45 CTCGGG C^[CT]CG[GA]G 4874 CCCGAG BbsI 547 GTCTTC GAAGAC(2/6) 4227 GTCTTC BbvCI 526 CCTCAGC CCTCAGC(-5/-2) 3827 CCTCAGC BpuAI 547 GTCTTC GAAGAC(2/6) 4227 GTCTTC BsaAI 2991 TACGTG [CT]AC^GT[GA] 3419 CACGTG BseAI 919 TCCGGA T^CCGGA 2659 TCCGGA BsiEI 3645 CGGCCG CG[GA][CT]^CG 4923 CGGCCG BsoBI 45 CTCGGG C^[CT]CG[GA]G 4874 CCCGAG BspEI 919 TCCGGA T^CCGGA 2659 TCCGGA BsrDI 4238 CATTGC GCAATG(2/0) 5184 CATTGC BstYI 1362 AGATCT [GA]^GATC[CT] 2789 GGATCC DraIII 1596 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 4163 CACATAGTG EagI 3645 CGGCCG C^GGCCG 4923 CGGCCG EclXI 3645 CGGCCG C^GGCCG 4923 CGGCCG Eco52I 3645 CGGCCG C^GGCCG 4923 CGGCCG EcoRV 1338 GATATC GAT^ATC 4047 GATATC EcoT22I 972 ATGCAT ATGCA^T 2352 ATGCAT FauI 655 GCGGG CCCGC(4/6) 4887 GCGGG HincII 2834 GTTGAC GT[CT]^[GA]AC 4173 GTTGAC HindII 2834 GTTGAC GT[CT]^[GA]AC 4173 GTTGAC MflI 1362 AGATCT [GA]^GATC[CT] 2789 GGATCC MroI 919 TCCGGA T^CCGGA 2659 TCCGGA NaeI 663 GCCGGC GCC^GGC 5113 GCCGGC NdeI 3521 CATATG CA^TATG 4431 CATATG NgoMIV 663 GCCGGC G^CCGGC 5113 GCCGGC NsiI 972 ATGCAT ATGCA^T 2352 ATGCAT PflFI 3734 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3902 GACGAGGTC PshBI 2732 ATTAAT AT^TAAT 3220 ATTAAT PstI 1881 CTGCAG CTGCA^G 4009 CTGCAG SacI 401 GAGCTC GAGCT^C 4918 GAGCTC ScaI 160 AGTACT AGT^ACT 1509 AGTACT SexAI 149 ACCTGGT A^CC[AT]GGT 1129 ACCAGGT Tth111I 3734 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3902 GACGAGGTC XhoII 1362 AGATCT [GA]^GATC[CT] 2789 GGATCC XmnI 130 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1441 GAAACTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BalI BanII BbvI BfaI BmyI BpmI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI Cac8I Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI