##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AccII AflII AflIII AgeI
Aor51HI AscI Asp718I AviII AvrII
BanI BbeI BciVI BfrI BlnI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII BssSI BstEII BstPI
BstUI CfoI ClaI CpoI DrdI
Eco47III EcoO65I EcoRI FspI HaeII
HhaI HinP1I HpaI KasI KpnI
KspI MluI MvnI NarI NheI
NotI NruI PacI PaeR7I PinAI
PmeI PshAI PvuI RcaI RsrII
SacII SalI SapI SbfI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SspBI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 3447 GACGTC GACGT^C
AclI 502 AACGTT AA^CGTT
AcyI 3447 GACGTC G[GA]^CG[CT]C
AhdI 3442 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3453 GTGCAC G^TGCAC
AocI 2015 CCTGAGG CC^T[ACGT]AGG
ApaLI 3453 GTGCAC G^TGCAC
AspEI 3442 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2789 GGATCC G^GATCC
BbrPI 3419 CACGTG CAC^GTG
BcgI 699 GCACTTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3776 TGATCA T^GATCA
BcnI 3643 CCCGG CC^[GC]GG
BglI 2250 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1362 AGATCT A^GATCT
BlpI 2651 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2651 GCTCAGC GC^T[ACGT]AGC
BsaBI 375 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3447 GACGTC G[GA]^CG[CT]C
BsrBI 4852 GAGCGG CCGCTC(-3/-3)
Bst1107I 3016 GTATAC GTA^TAC
BstBI 238 TTCGAA TT^CGAA
BstZ17I 3016 GTATAC GTA^TAC
Bsu36I 2015 CCTGAGG CC^T[ACGT]AGG
CelII 2651 GCTCAGC GC^T[ACGT]AGC
Eam1105I 3442 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2015 CCTGAGG CC^T[ACGT]AGG
FbaI 3776 TGATCA T^GATCA
FseI 5112 GGCCGGCC GGCCGG^CC
Hin1I 3447 GACGTC G[GA]^CG[CT]C
HindIII 4493 AAGCTT A^AGCTT
MamI 375 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2045 CAATTG C^AATTG
MunI 2045 CAATTG C^AATTG
NciI 3643 CCCGG CC^[GC]GG
NspV 238 TTCGAA TT^CGAA
PmaCI 3419 CACGTG CAC^GTG
PmlI 3419 CACGTG CAC^GTG
Psp1406I 502 AACGTT AA^CGTT
SfuI 238 TTCGAA TT^CGAA
SpeI 2515 ACTAGT A^CTAGT
SphI 1207 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 919 TCCGGA T^CCGGA
2659 TCCGGA
ApaI 935 GGGCCC GGGCC^C
3640 GGGCCC
AseI 2732 ATTAAT AT^TAAT
3220 ATTAAT
AsnI 2732 ATTAAT AT^TAAT
3220 ATTAAT
Asp700I 130 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1441 GAAACTATTC
AspI 3734 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3902 GACGAGGTC
AvaI 45 CTCGGG C^[CT]CG[GA]G
4874 CCCGAG
BbsI 547 GTCTTC GAAGAC(2/6)
4227 GTCTTC
BbvCI 526 CCTCAGC CCTCAGC(-5/-2)
3827 CCTCAGC
BpuAI 547 GTCTTC GAAGAC(2/6)
4227 GTCTTC
BsaAI 2991 TACGTG [CT]AC^GT[GA]
3419 CACGTG
BseAI 919 TCCGGA T^CCGGA
2659 TCCGGA
BsiEI 3645 CGGCCG CG[GA][CT]^CG
4923 CGGCCG
BsoBI 45 CTCGGG C^[CT]CG[GA]G
4874 CCCGAG
BspEI 919 TCCGGA T^CCGGA
2659 TCCGGA
BsrDI 4238 CATTGC GCAATG(2/0)
5184 CATTGC
BstYI 1362 AGATCT [GA]^GATC[CT]
2789 GGATCC
DraIII 1596 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4163 CACATAGTG
EagI 3645 CGGCCG C^GGCCG
4923 CGGCCG
EclXI 3645 CGGCCG C^GGCCG
4923 CGGCCG
Eco52I 3645 CGGCCG C^GGCCG
4923 CGGCCG
EcoRV 1338 GATATC GAT^ATC
4047 GATATC
EcoT22I 972 ATGCAT ATGCA^T
2352 ATGCAT
FauI 655 GCGGG CCCGC(4/6)
4887 GCGGG
HincII 2834 GTTGAC GT[CT]^[GA]AC
4173 GTTGAC
HindII 2834 GTTGAC GT[CT]^[GA]AC
4173 GTTGAC
MflI 1362 AGATCT [GA]^GATC[CT]
2789 GGATCC
MroI 919 TCCGGA T^CCGGA
2659 TCCGGA
NaeI 663 GCCGGC GCC^GGC
5113 GCCGGC
NdeI 3521 CATATG CA^TATG
4431 CATATG
NgoMIV 663 GCCGGC G^CCGGC
5113 GCCGGC
NsiI 972 ATGCAT ATGCA^T
2352 ATGCAT
PflFI 3734 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3902 GACGAGGTC
PshBI 2732 ATTAAT AT^TAAT
3220 ATTAAT
PstI 1881 CTGCAG CTGCA^G
4009 CTGCAG
SacI 401 GAGCTC GAGCT^C
4918 GAGCTC
ScaI 160 AGTACT AGT^ACT
1509 AGTACT
SexAI 149 ACCTGGT A^CC[AT]GGT
1129 ACCAGGT
Tth111I 3734 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3902 GACGAGGTC
XhoII 1362 AGATCT [GA]^GATC[CT]
2789 GGATCC
XmnI 130 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1441 GAAACTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanII BbvI BfaI BmyI
BpmI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI Cac8I Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XcmI