##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

Acc65I      AccII       AflII       AflIII      AgeI        
Aor51HI     AscI        Asp718I     AviII       AvrII       
BanI        BbeI        BciVI       BfrI        BlnI        
BsiWI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrGI       BssHII      BssSI       BstEII      BstPI       
BstUI       CfoI        ClaI        CpoI        DrdI        
Eco47III    EcoO65I     EcoRI       FspI        HaeII       
HhaI        HinP1I      HpaI        KasI        KpnI        
KspI        MluI        MvnI        NarI        NheI        
NotI        NruI        PacI        PaeR7I      PinAI       
PmeI        PshAI       PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        SfiI        
SfoI        SgrAI       SmaI        SnaBI       Sse8387I    
SspBI       SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII        3447  GACGTC               GACGT^C
AclI          502  AACGTT               AA^CGTT
AcyI         3447  GACGTC               G[GA]^CG[CT]C
AhdI         3442  GACAAGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       3453  GTGCAC               G^TGCAC
AocI         2015  CCTGAGG              CC^T[ACGT]AGG
ApaLI        3453  GTGCAC               G^TGCAC
AspEI        3442  GACAAGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        2789  GGATCC               G^GATCC
BbrPI        3419  CACGTG               CAC^GTG
BcgI          699  GCACTTGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         3776  TGATCA               T^GATCA
BcnI         3643  CCCGG                CC^[GC]GG
BglI         2250  GCCACTTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1362  AGATCT               A^GATCT
BlpI         2651  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2651  GCTCAGC              GC^T[ACGT]AGC
BsaBI         375  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        3447  GACGTC               G[GA]^CG[CT]C
BsrBI        4852  GAGCGG               CCGCTC(-3/-3)
Bst1107I     3016  GTATAC               GTA^TAC
BstBI         238  TTCGAA               TT^CGAA
BstZ17I      3016  GTATAC               GTA^TAC
Bsu36I       2015  CCTGAGG              CC^T[ACGT]AGG
CelII        2651  GCTCAGC              GC^T[ACGT]AGC
Eam1105I     3442  GACAAGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2015  CCTGAGG              CC^T[ACGT]AGG
FbaI         3776  TGATCA               T^GATCA
FseI         5112  GGCCGGCC             GGCCGG^CC
Hin1I        3447  GACGTC               G[GA]^CG[CT]C
HindIII      4493  AAGCTT               A^AGCTT
MamI          375  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2045  CAATTG               C^AATTG
MunI         2045  CAATTG               C^AATTG
NciI         3643  CCCGG                CC^[GC]GG
NspV          238  TTCGAA               TT^CGAA
PmaCI        3419  CACGTG               CAC^GTG
PmlI         3419  CACGTG               CAC^GTG
Psp1406I      502  AACGTT               AA^CGTT
SfuI          238  TTCGAA               TT^CGAA
SpeI         2515  ACTAGT               A^CTAGT
SphI         1207  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   41 enzymes) #
###################################

AccIII        919  TCCGGA               T^CCGGA
             2659  TCCGGA              
ApaI          935  GGGCCC               GGGCC^C
             3640  GGGCCC              
AseI         2732  ATTAAT               AT^TAAT
             3220  ATTAAT              
AsnI         2732  ATTAAT               AT^TAAT
             3220  ATTAAT              
Asp700I       130  GAACCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1441  GAAACTATTC          
AspI         3734  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3902  GACGAGGTC           
AvaI           45  CTCGGG               C^[CT]CG[GA]G
             4874  CCCGAG              
BbsI          547  GTCTTC               GAAGAC(2/6)
             4227  GTCTTC              
BbvCI         526  CCTCAGC              CCTCAGC(-5/-2)
             3827  CCTCAGC             
BpuAI         547  GTCTTC               GAAGAC(2/6)
             4227  GTCTTC              
BsaAI        2991  TACGTG               [CT]AC^GT[GA]
             3419  CACGTG              
BseAI         919  TCCGGA               T^CCGGA
             2659  TCCGGA              
BsiEI        3645  CGGCCG               CG[GA][CT]^CG
             4923  CGGCCG              
BsoBI          45  CTCGGG               C^[CT]CG[GA]G
             4874  CCCGAG              
BspEI         919  TCCGGA               T^CCGGA
             2659  TCCGGA              
BsrDI        4238  CATTGC               GCAATG(2/0)
             5184  CATTGC              
BstYI        1362  AGATCT               [GA]^GATC[CT]
             2789  GGATCC              
DraIII       1596  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4163  CACATAGTG           
EagI         3645  CGGCCG               C^GGCCG
             4923  CGGCCG              
EclXI        3645  CGGCCG               C^GGCCG
             4923  CGGCCG              
Eco52I       3645  CGGCCG               C^GGCCG
             4923  CGGCCG              
EcoRV        1338  GATATC               GAT^ATC
             4047  GATATC              
EcoT22I       972  ATGCAT               ATGCA^T
             2352  ATGCAT              
FauI          655  GCGGG                CCCGC(4/6)
             4887  GCGGG               
HincII       2834  GTTGAC               GT[CT]^[GA]AC
             4173  GTTGAC              
HindII       2834  GTTGAC               GT[CT]^[GA]AC
             4173  GTTGAC              
MflI         1362  AGATCT               [GA]^GATC[CT]
             2789  GGATCC              
MroI          919  TCCGGA               T^CCGGA
             2659  TCCGGA              
NaeI          663  GCCGGC               GCC^GGC
             5113  GCCGGC              
NdeI         3521  CATATG               CA^TATG
             4431  CATATG              
NgoMIV        663  GCCGGC               G^CCGGC
             5113  GCCGGC              
NsiI          972  ATGCAT               ATGCA^T
             2352  ATGCAT              
PflFI        3734  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3902  GACGAGGTC           
PshBI        2732  ATTAAT               AT^TAAT
             3220  ATTAAT              
PstI         1881  CTGCAG               CTGCA^G
             4009  CTGCAG              
SacI          401  GAGCTC               GAGCT^C
             4918  GAGCTC              
ScaI          160  AGTACT               AGT^ACT
             1509  AGTACT              
SexAI         149  ACCTGGT              A^CC[AT]GGT
             1129  ACCAGGT             
Tth111I      3734  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3902  GACGAGGTC           
XhoII        1362  AGATCT               [GA]^GATC[CT]
             2789  GGATCC              
XmnI          130  GAACCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1441  GAAACTATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaII       
BalI        BanII       BbvI        BfaI        BmyI        
BpmI        BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstXI       Cac8I       Cfr10I      Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HgaI        HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XbaI        XcmI