##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AspEI AspI AviII AvrII
BalI BbeI BbrPI BglI BlnI
BlpI Bpu1102I BsaHI BseAI BsiEI
BsiWI Bsp1407I BspEI BsrGI BssHII
Bsu36I CelII CpoI EagI Eam1105I
EclXI Eco52I Eco81I FseI FspI
Hin1I KasI KspI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NgoMIV NotI NruI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI Sse8387I SspBI
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 359 GGTACC G^GTACC
Aor51HI 2053 AGCGCT AGC^GCT
Asp700I 3852 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 359 GGTACC G^GTACC
BbvCI 3054 GCTGAGG CCTCAGC(-5/-2)
BcgI 2351 CGATGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3938 TGATCA T^GATCA
BglII 756 AGATCT A^GATCT
BsaAI 3973 TACGTA [CT]AC^GT[GA]
BsaI 3219 GAGACC GGTCTC(1/5)
BsaWI 2962 TCCGGT [AT]^CCGG[AT]
BsmBI 1591 CGTCTC CGTCTC(1/5)
BspDI 1084 ATCGAT AT^CGAT
BsrBI 2192 CCGCTC CCGCTC(-3/-3)
BssSI 2026 CTCGTG CACGAG(-5/-1)
Bst1107I 3457 GTATAC GTA^TAC
BstBI 2436 TTCGAA TT^CGAA
BstEII 4472 GGTTACC G^GT[ACGT]ACC
BstPI 4472 GGTTACC G^GT[ACGT]ACC
BstXI 1052 CCAGAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3457 GTATAC GTA^TAC
ClaI 1084 ATCGAT AT^CGAT
DraIII 3231 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4226 GACATTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2801 TGGCCG [CT]^GGCC[GA]
Eco47III 2053 AGCGCT AGC^GCT
EcoNI 1519 CCTATGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4472 GGTTACC G^GT[ACGT]ACC
EcoRV 2149 GATATC GAT^ATC
EcoT22I 3955 ATGCAT ATGCA^T
FbaI 3938 TGATCA T^GATCA
HaeII 2053 AGCGCT [GA]GCGC^[CT]
HindIII 1467 AAGCTT A^AGCTT
HpaI 66 GTTAAC GTT^AAC
KpnI 359 GGTACC GGTAC^C
NheI 17 GCTAGC G^CTAGC
NsiI 3955 ATGCAT ATGCA^T
NspV 2436 TTCGAA TT^CGAA
PaeR7I 541 CTCGAG C^TCGAG
SacI 2604 GAGCTC GAGCT^C
SapI 802 GAAGAGC GCTCTTC(1/4)
SfuI 2436 TTCGAA TT^CGAA
SnaBI 3973 TACGTA TAC^GTA
SpeI 4142 ACTAGT A^CTAGT
SphI 2710 GCATGC GCATG^C
XhoI 541 CTCGAG C^TCGAG
XmnI 3852 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 8 CGCG CG^CG
160 CGCG
AflII 1637 CTTAAG C^TTAAG
3178 CTTAAG
AvaI 541 CTCGAG C^[CT]CG[GA]G
2812 CCCGAG
BamHI 2368 GGATCC G^GATCC
2882 GGATCC
BanII 1453 GGGCTC G[GA]GC[CT]^C
2604 GAGCTC
BciVI 3285 GTATCC GTATCC(6/5)
5334 GTATCC
BfrI 1637 CTTAAG C^TTAAG
3178 CTTAAG
BpmI 760 CTGGAG CTGGAG(16/14)
3216 CTGGAG
BsaBI 1196 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5511 GATGCTCATC
BsoBI 541 CTCGAG C^[CT]CG[GA]G
2812 CCCGAG
BspHI 4985 TCATGA T^CATGA
5793 TCATGA
BspLU11I 70 ACATGT A^CATGT
4523 ACATGT
BsrFI 2454 ACCGGC [GA]^CCGG[CT]
2803 GCCGGT
BstUI 8 CGCG CG^CG
160 CGCG
CfoI 9 GCGC GCG^C
2054 GCGC
Cfr10I 2454 ACCGGC [GA]^CCGG[CT]
2803 GCCGGT
DraII 3297 AGGACCT [GA]G^G[ACGT]CC[CT]
5003 AGGACCC
EcoO109I 3297 AGGACCT [GA]G^G[ACGT]CC[CT]
5003 AGGACCC
FauI 161 GCGGG CCCGC(4/6)
1574 GCGGG
HhaI 9 GCGC GCG^C
2054 GCGC
HinP1I 9 GCGC G^CGC
2054 GCGC
HincII 66 GTTAAC GT[CT]^[GA]AC
4948 GTCAAC
HindII 66 GTTAAC GT[CT]^[GA]AC
4948 GTCAAC
MamI 1196 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5511 GATGCTCATC
MvnI 8 CGCG CG^CG
160 CGCG
NcoI 3163 CCATGG C^CATGG
5297 CCATGG
NdeI 1096 CATATG CA^TATG
4786 CATATG
PpuMI 3297 AGGACCT [GA]G^G[AT]CC[CT]
5003 AGGACCC
PvuII 699 CAGCTG CAG^CTG
3000 CAGCTG
RcaI 4985 TCATGA T^CATGA
5793 TCATGA
SspI 3863 AATATT AAT^ATT
4925 AATATT
StuI 3268 AGGCCT AGG^CCT
4482 AGGCCT
SwaI 404 ATTTAAAT ATTT^AAAT
4920 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaII BanI BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII