##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AgeI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AviII
AvrII BbeI BbsI BcgI BciVI
BlnI BlpI Bpu1102I BpuAI BsaHI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FseI FspI HgaI Hin1I
KasI KspI MluI MroI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NspV PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 3835 GGTACC G^GTACC
AflII 1528 CTTAAG C^TTAAG
AflIII 1325 ACACGT A^C[GA][CT]GT
AocI 3948 CCTAAGG CC^T[ACGT]AGG
Asp718I 3835 GGTACC G^GTACC
AspI 2323 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 610 CCCGGG C^[CT]CG[GA]G
BamHI 442 GGATCC G^GATCC
BbrPI 2457 CACGTG CAC^GTG
BclI 1981 TGATCA T^GATCA
BfrI 1528 CTTAAG C^TTAAG
BglI 2591 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2457 CACGTG [CT]AC^GT[GA]
BsaBI 1761 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3067 GGTCTC GGTCTC(1/5)
BsoBI 610 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1586 TGTACA T^GTACA
BsrBI 2670 GAGCGG CCGCTC(-3/-3)
BsrGI 1586 TGTACA T^GTACA
BstAPI 4752 GCAATGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3948 CCTAAGG CC^T[ACGT]AGG
CfoI 704 GCGC GCG^C
DraI 2961 TTTAAA TTT^AAA
DraIII 2454 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 867 GACAATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3948 CCTAAGG CC^T[ACGT]AGG
FbaI 1981 TGATCA T^GATCA
HaeII 703 GGCGCT [GA]GCGC^[CT]
HapII 611 CCGG C^CGG
HhaI 704 GCGC GCG^C
HinP1I 704 GCGC G^CGC
HindIII 2614 AAGCTT A^AGCTT
HpaII 611 CCGG C^CGG
KpnI 3835 GGTACC GGTAC^C
MamI 1761 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 5303 CAGCTG C[AC]G^C[GT]G
MspI 611 CCGG C^CGG
PacI 1407 TTAATTAA TTAAT^TAA
PflFI 2323 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 5299 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2457 CACGTG CAC^GTG
PmlI 2457 CACGTG CAC^GTG
PshAI 1166 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 5303 CAGCTG CAG^CTG
SacI 5264 GAGCTC GAGCT^C
ScaI 4560 AGTACT AGT^ACT
SmaI 610 CCCGGG CCC^GGG
SspBI 1586 TGTACA T^GTACA
TaqI 1017 TCGA T^CGA
Tth111I 2323 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1017 TCGA T^CGA
Van91I 5299 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 610 CCCGGG C^CCGGG
XmaI 610 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AhdI 522 GACATTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3322 GACTCTCAGTC
AspEI 522 GACATTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3322 GACTCTCAGTC
BalI 2510 TGGCCA TGG^CCA
3341 TGGCCA
BcnI 610 CCCGG CC^[GC]GG
611 CCGGG
BsmBI 2321 GAGACG CGTCTC(1/5)
2490 GAGACG
BspHI 3863 TCATGA T^CATGA
4034 TCATGA
DsaI 2884 CCATGG C^C[GA][CT]GG
3705 CCATGG
EaeI 2510 TGGCCA [CT]^GGCC[GA]
3341 TGGCCA
Eam1105I 522 GACATTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3322 GACTCTCAGTC
EarI 1537 CTCTTC CTCTTC(1/4)
417 GAAGAG
EcoT22I 92 ATGCAT ATGCA^T
1234 ATGCAT
FauI 1716 GCGGG CCCGC(4/6)
2672 GCGGG
HpaI 227 GTTAAC GTT^AAC
3374 GTTAAC
Ksp632I 1537 CTCTTC CTCTTC(1/4)
417 GAAGAG
MfeI 3171 CAATTG C^AATTG
5159 CAATTG
MluNI 2510 TGGCCA TGG^CCA
3341 TGGCCA
MscI 2510 TGGCCA TGG^CCA
3341 TGGCCA
MunI 3171 CAATTG C^AATTG
5159 CAATTG
NciI 610 CCCGG CC^[GC]GG
611 CCGGG
NcoI 2884 CCATGG C^CATGG
3705 CCATGG
NdeI 1777 CATATG CA^TATG
3789 CATATG
NsiI 92 ATGCAT ATGCA^T
1234 ATGCAT
RcaI 3863 TCATGA T^CATGA
4034 TCATGA
SbfI 3779 CCTGCAGG CCTGCA^GG
4471 CCTGCAGG
SexAI 3911 ACCTGGT A^CC[AT]GGT
3939 ACCAGGT
Sse8387I 3779 CCTGCAGG CCTGCA^GG
4471 CCTGCAGG
SspI 3305 AATATT AAT^ATT
5355 AATATT
XbaI 806 TCTAGA T^CTAGA
1842 TCTAGA
XcmI 391 CCATCAATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4851 CCACCTGAGCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanI BanII BbvCI BbvI BfaI
BglII BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BssSI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI