################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AviII AvrII BbeI BbsI BcgI BciVI BlnI BlpI Bpu1102I BpuAI BsaHI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FseI FspI HgaI Hin1I KasI KspI MluI MroI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SapI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SwaI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 3835 GGTACC G^GTACC AflII 1528 CTTAAG C^TTAAG AflIII 1325 ACACGT A^C[GA][CT]GT AocI 3948 CCTAAGG CC^T[ACGT]AGG Asp718I 3835 GGTACC G^GTACC AspI 2323 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 610 CCCGGG C^[CT]CG[GA]G BamHI 442 GGATCC G^GATCC BbrPI 2457 CACGTG CAC^GTG BclI 1981 TGATCA T^GATCA BfrI 1528 CTTAAG C^TTAAG BglI 2591 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 2457 CACGTG [CT]AC^GT[GA] BsaBI 1761 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 3067 GGTCTC GGTCTC(1/5) BsoBI 610 CCCGGG C^[CT]CG[GA]G Bsp1407I 1586 TGTACA T^GTACA BsrBI 2670 GAGCGG CCGCTC(-3/-3) BsrGI 1586 TGTACA T^GTACA BstAPI 4752 GCAATGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 3948 CCTAAGG CC^T[ACGT]AGG CfoI 704 GCGC GCG^C DraI 2961 TTTAAA TTT^AAA DraIII 2454 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 867 GACAATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3948 CCTAAGG CC^T[ACGT]AGG FbaI 1981 TGATCA T^GATCA HaeII 703 GGCGCT [GA]GCGC^[CT] HapII 611 CCGG C^CGG HhaI 704 GCGC GCG^C HinP1I 704 GCGC G^CGC HindIII 2614 AAGCTT A^AGCTT HpaII 611 CCGG C^CGG KpnI 3835 GGTACC GGTAC^C MamI 1761 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 5303 CAGCTG C[AC]G^C[GT]G MspI 611 CCGG C^CGG PacI 1407 TTAATTAA TTAAT^TAA PflFI 2323 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 5299 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2457 CACGTG CAC^GTG PmlI 2457 CACGTG CAC^GTG PshAI 1166 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 5303 CAGCTG CAG^CTG SacI 5264 GAGCTC GAGCT^C ScaI 4560 AGTACT AGT^ACT SmaI 610 CCCGGG CCC^GGG SspBI 1586 TGTACA T^GTACA TaqI 1017 TCGA T^CGA Tth111I 2323 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1017 TCGA T^CGA Van91I 5299 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 610 CCCGGG C^CCGGG XmaI 610 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AhdI 522 GACATTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3322 GACTCTCAGTC AspEI 522 GACATTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3322 GACTCTCAGTC BalI 2510 TGGCCA TGG^CCA 3341 TGGCCA BcnI 610 CCCGG CC^[GC]GG 611 CCGGG BsmBI 2321 GAGACG CGTCTC(1/5) 2490 GAGACG BspHI 3863 TCATGA T^CATGA 4034 TCATGA DsaI 2884 CCATGG C^C[GA][CT]GG 3705 CCATGG EaeI 2510 TGGCCA [CT]^GGCC[GA] 3341 TGGCCA Eam1105I 522 GACATTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3322 GACTCTCAGTC EarI 1537 CTCTTC CTCTTC(1/4) 417 GAAGAG EcoT22I 92 ATGCAT ATGCA^T 1234 ATGCAT FauI 1716 GCGGG CCCGC(4/6) 2672 GCGGG HpaI 227 GTTAAC GTT^AAC 3374 GTTAAC Ksp632I 1537 CTCTTC CTCTTC(1/4) 417 GAAGAG MfeI 3171 CAATTG C^AATTG 5159 CAATTG MluNI 2510 TGGCCA TGG^CCA 3341 TGGCCA MscI 2510 TGGCCA TGG^CCA 3341 TGGCCA MunI 3171 CAATTG C^AATTG 5159 CAATTG NciI 610 CCCGG CC^[GC]GG 611 CCGGG NcoI 2884 CCATGG C^CATGG 3705 CCATGG NdeI 1777 CATATG CA^TATG 3789 CATATG NsiI 92 ATGCAT ATGCA^T 1234 ATGCAT RcaI 3863 TCATGA T^CATGA 4034 TCATGA SbfI 3779 CCTGCAGG CCTGCA^GG 4471 CCTGCAGG SexAI 3911 ACCTGGT A^CC[AT]GGT 3939 ACCAGGT Sse8387I 3779 CCTGCAGG CCTGCA^GG 4471 CCTGCAGG SspI 3305 AATATT AAT^ATT 5355 AATATT XbaI 806 TCTAGA T^CTAGA 1842 TCTAGA XcmI 391 CCATCAATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4851 CCACCTGAGCTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BanI BanII BbvCI BbvI BfaI BglII BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI