##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI Alw44I ApaI ApaLI AscI
Asp718I AspEI AspI AviII BanI
BbeI BcgI BglI BglII BsaBI
BsaHI BsiEI BsiWI Bsp1407I BspDI
BspHI BsrFI BsrGI BssSI Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoRI EcoRV
FseI FspI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NaeI NarI NgoMIV NheI
NotI NruI PacI PflFI PflMI
PinAI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SnaBI
Sse8387I SspBI SwaI Tth111I Van91I
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccI 2045 GTATAC GT^[AC][GT]AC
AccIII 1088 TCCGGA T^CCGGA
AflII 2316 CTTAAG C^TTAAG
AocI 3316 CCTTAGG CC^T[ACGT]AGG
AseI 733 ATTAAT AT^TAAT
AsnI 733 ATTAAT AT^TAAT
Asp700I 1102 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1198 GAGCTC G[AT]GC[AT]^C
AvrII 2841 CCTAGG C^CTAGG
BbrPI 2521 CACGTG CAC^GTG
BbsI 2605 GTCTTC GAAGAC(2/6)
BbvCI 3448 CCTCAGC CCTCAGC(-5/-2)
BclI 1518 TGATCA T^GATCA
BfrI 2316 CTTAAG C^TTAAG
BlnI 2841 CCTAGG C^CTAGG
BpuAI 2605 GTCTTC GAAGAC(2/6)
BsaAI 2521 CACGTG [CT]AC^GT[GA]
BsaWI 1088 TCCGGA [AT]^CCGG[AT]
BseAI 1088 TCCGGA T^CCGGA
BsgI 2575 GTGCAG GTGCAG(16/14)
BsiHKAI 1198 GAGCTC G[AT]GC[AT]^C
BsmBI 77 CGTCTC CGTCTC(1/5)
BspEI 1088 TCCGGA T^CCGGA
BspLU11I 2415 ACATGT A^CATGT
BsrBI 125 GAGCGG CCGCTC(-3/-3)
BsrDI 1259 GCAATG GCAATG(2/0)
BssHII 84 GCGCGC G^CGCGC
Bst1107I 2045 GTATAC GTA^TAC
BstAPI 482 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1214 TTCGAA TT^CGAA
BstEII 2848 GGTCACC G^GT[ACGT]ACC
BstPI 2848 GGTCACC G^GT[ACGT]ACC
BstXI 111 CCAGCGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2045 GTATAC GTA^TAC
Bsu36I 3316 CCTTAGG CC^T[ACGT]AGG
DraII 3220 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 671 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3316 CCTTAGG CC^T[ACGT]AGG
EcoO109I 3220 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2848 GGTCACC G^GT[ACGT]ACC
FbaI 1518 TGATCA T^GATCA
HgaI 711 GCGTC GACGC(5/10)
HincII 532 GTTGAC GT[CT]^[GA]AC
HindII 532 GTTGAC GT[CT]^[GA]AC
HindIII 3082 AAGCTT A^AGCTT
MroI 1088 TCCGGA T^CCGGA
NcoI 278 CCATGG C^CATGG
NdeI 2600 CATATG CA^TATG
NspV 1214 TTCGAA TT^CGAA
PaeR7I 2225 CTCGAG C^TCGAG
PmaCI 2521 CACGTG CAC^GTG
PmeI 2377 GTTTAAAC GTTT^AAAC
PmlI 2521 CACGTG CAC^GTG
PpuMI 3220 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 733 ATTAAT AT^TAAT
SacI 1198 GAGCTC GAGCT^C
SfuI 1214 TTCGAA TT^CGAA
SmaI 1366 CCCGGG CCC^GGG
SphI 1382 GCATGC GCATG^C
SspI 736 AATATT AAT^ATT
XhoI 2225 CTCGAG C^TCGAG
XmaCI 1366 CCCGGG C^CCGGG
XmaI 1366 CCCGGG C^CCGGG
XmnI 1102 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 85 CGCG CG^CG
178 CGCG
AflIII 1970 ACACGT A^C[GA][CT]GT
2415 ACATGT
Aor51HI 295 AGCGCT AGC^GCT
1415 AGCGCT
AvaI 1366 CCCGGG C^[CT]CG[GA]G
2225 CTCGAG
AvaII 1086 GGTCC G^G[AT]CC
3221 GGACC
BalI 275 TGGCCA TGG^CCA
281 TGGCCA
BanII 150 GAGCCC G[GA]GC[CT]^C
1198 GAGCTC
BciVI 2526 GGATAC GTATCC(6/5)
3147 GTATCC
BlpI 122 GCTGAGC GC^T[ACGT]AGC
1411 GCTCAGC
Bpu1102I 122 GCTGAGC GC^T[ACGT]AGC
1411 GCTCAGC
BseRI 364 GAGGAG GAGGAG(10/8)
1318 GAGGAG
BsmI 418 GAATGC GAATGC(1/-1)
1295 GAATGC
BsoBI 1366 CCCGGG C^[CT]CG[GA]G
2225 CTCGAG
BspMI 514 GCAGGT ACCTGC(4/8)
2925 GCAGGT
BstUI 85 CGCG CG^CG
178 CGCG
CelII 122 GCTGAGC GC^T[ACGT]AGC
1411 GCTCAGC
Eco47III 295 AGCGCT AGC^GCT
1415 AGCGCT
EcoT22I 1297 ATGCAT ATGCA^T
1642 ATGCAT
MluNI 275 TGGCCA TGG^CCA
281 TGGCCA
MscI 275 TGGCCA TGG^CCA
281 TGGCCA
MspA1I 237 CAGCTG C[AC]G^C[GT]G
3451 CAGCTG
MvnI 85 CGCG CG^CG
178 CGCG
NsiI 1297 ATGCAT ATGCA^T
1642 ATGCAT
PstI 2923 CTGCAG CTGCA^G
3230 CTGCAG
PvuII 237 CAGCTG CAG^CTG
3451 CAGCTG
SpeI 1890 ACTAGT A^CTAGT
3074 ACTAGT
StuI 1998 AGGCCT AGG^CCT
3019 AGGCCT
XcmI 350 CCAGCTCATTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
425 CCATCTGCTTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BamHI BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII