##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AflIII AgeI AhdI
Alw44I AocI ApaLI AseI AsnI
AspEI BbrPI BcgI BciVI BsaBI
BsaWI BseAI BspDI BspEI BspLU11I
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraIII
Eam1105I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI HindIII HpaI KspI
MamI MfeI MluI MroI MunI
NheI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SalI
SapI SbfI ScaI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AccI 3526 GTAGAC GT^[AC][GT]AC
AscI 1235 GGCGCGCC GG^CGCGCC
Asp700I 3549 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2310 TGCGCA TGC^GCA
BclI 1987 TGATCA T^GATCA
BglII 4195 AGATCT A^GATCT
BsaAI 4549 TACGTG [CT]AC^GT[GA]
BsiWI 2289 CGTACG C^GTACG
BspHI 3518 TCATGA T^CATGA
BstXI 4730 CCAACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2719 TTTAAA TTT^AAA
DrdI 1644 GACGGCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1960 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1987 TGATCA T^GATCA
FspI 2310 TGCGCA TGC^GCA
HincII 4628 GTCAAC GT[CT]^[GA]AC
HindII 4628 GTCAAC GT[CT]^[GA]AC
NcoI 2869 CCATGG C^CATGG
NdeI 2561 CATATG CA^TATG
NspI 2222 GCATGT [GA]CATG^[CT]
PpuMI 1188 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 3518 TCATGA T^CATGA
SacI 1040 GAGCTC GAGCT^C
SexAI 504 ACCTGGT A^CC[AT]GGT
XbaI 2006 TCTAGA T^CTAGA
XmnI 3549 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1860 GGTACC G^GTACC
4701 GGTACC
AclI 3258 AACGTT AA^CGTT
4631 AACGTT
AflII 2833 CTTAAG C^TTAAG
2915 CTTAAG
Aor51HI 179 AGCGCT AGC^GCT
2478 AGCGCT
Asp718I 1860 GGTACC G^GTACC
4701 GGTACC
AspI 2123 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3747 GACGGTGTC
BamHI 1730 GGATCC G^GATCC
2495 GGATCC
BbeI 195 GGCGCC GGCGC^C
620 GGCGCC
BbvCI 832 CCTCAGC CCTCAGC(-5/-2)
3839 CCTCAGC
BfrI 2833 CTTAAG C^TTAAG
2915 CTTAAG
BlpI 172 GCTGAGC GC^T[ACGT]AGC
1081 GCTCAGC
Bpu1102I 172 GCTGAGC GC^T[ACGT]AGC
1081 GCTCAGC
BsmBI 533 GAGACG CGTCTC(1/5)
3745 GAGACG
BspMI 2855 ACCTGC ACCTGC(4/8)
1477 GCAGGT
BstAPI 1258 GCAAGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1784 GCAGTCTCTGC
CelII 172 GCTGAGC GC^T[ACGT]AGC
1081 GCTCAGC
DsaI 2869 CCATGG C^C[GA][CT]GG
3338 CCGTGG
Eco47III 179 AGCGCT AGC^GCT
2478 AGCGCT
KasI 195 GGCGCC G^GCGCC
620 GGCGCC
KpnI 1860 GGTACC GGTAC^C
4701 GGTACC
NaeI 192 GCCGGC GCC^GGC
1397 GCCGGC
NarI 195 GGCGCC GG^CGCC
620 GGCGCC
NgoMIV 192 GCCGGC G^CCGGC
1397 GCCGGC
NotI 424 GCGGCCGC GC^GGCCGC
545 GCGGCCGC
PaeR7I 1951 CTCGAG C^TCGAG
3152 CTCGAG
PflFI 2123 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3747 GACGGTGTC
Psp1406I 3258 AACGTT AA^CGTT
4631 AACGTT
PvuII 773 CAGCTG CAG^CTG
1282 CAGCTG
SfiI 484 GGCCAGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1828 GGCCTGTGTGGCC
SfoI 195 GGCGCC GGC^GCC
620 GGCGCC
Tth111I 2123 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3747 GACGGTGTC
XhoI 1951 CTCGAG C^TCGAG
3152 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BlnI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI