##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AvrII BbeI BcgI BfrI BglII
BlnI BlpI Bpu1102I BsaWI BseAI
BsgI BsiWI Bsp1407I BspDI BspEI
BspHI BsrDI BsrFI BsrGI BssHII
Bst1107I BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII Eco47III Eco81I EcoO65I EcoRV
EcoT22I FseI HaeII HpaI KasI
KspI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PinAI PmeI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 217 GACGTC GACGT^C
Acc65I 1100 GGTACC G^GTACC
AccII 1 CGCG CG^CG
AcyI 217 GACGTC G[GA]^CG[CT]C
AhdI 2376 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 281 GGGCCC GGGCC^C
Asp718I 1100 GGTACC G^GTACC
AspEI 2376 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3817 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 890 TGCGCA TGC^GCA
BalI 3901 TGGCCA TGG^CCA
BbvCI 1059 CCTCAGC CCTCAGC(-5/-2)
BclI 2069 TGATCA T^GATCA
BglI 1503 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 3949 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 217 GACGTC G[GA]^CG[CT]C
BsiEI 204 CGGCCG CG[GA][CT]^CG
BsmI 367 GAATGC GAATGC(1/-1)
BspLU11I 3895 ACATGT A^CATGT
BsrBI 1575 GAGCGG CCGCTC(-3/-3)
BssSI 3227 CACGAG CACGAG(-5/-1)
BstAPI 1074 GCAATACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2312 TTCGAA TT^CGAA
BstUI 1 CGCG CG^CG
DraI 3662 TTTAAA TTT^AAA
DrdI 1727 GACAATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 204 CGGCCG C^GGCCG
Eam1105I 2376 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 204 CGGCCG C^GGCCG
Eco52I 204 CGGCCG C^GGCCG
EcoRI 3696 GAATTC G^AATTC
FbaI 2069 TGATCA T^GATCA
FspI 890 TGCGCA TGC^GCA
HgaI 3011 GACGC GACGC(5/10)
Hin1I 217 GACGTC G[GA]^CG[CT]C
HindIII 2781 AAGCTT A^AGCTT
KpnI 1100 GGTACC GGTAC^C
MamI 3949 GATCTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2236 CAATTG C^AATTG
MluNI 3901 TGGCCA TGG^CCA
MscI 3901 TGGCCA TGG^CCA
MunI 2236 CAATTG C^AATTG
MvnI 1 CGCG CG^CG
NdeI 3169 CATATG CA^TATG
NspV 2312 TTCGAA TT^CGAA
PflFI 3817 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 165 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2718 AGGACCC [GA]G^G[AT]CC[CT]
PstI 817 CTGCAG CTGCA^G
SacI 66 GAGCTC GAGCT^C
ScaI 3125 AGTACT AGT^ACT
SfuI 2312 TTCGAA TT^CGAA
SmaI 59 CCCGGG CCC^GGG
SmlI 92 CTTGAG C^T[CT][GA]AG
StuI 88 AGGCCT AGG^CCT
Tth111I 3817 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 165 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1915 CCATTGTCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 59 CCCGGG C^CCGGG
XmaI 59 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 474 ACACGT A^C[GA][CT]GT
3895 ACATGT
Asp700I 1940 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3378 GAACAGTTTC
AspHI 66 GAGCTC G[AT]GC[AT]^C
2826 GAGCAC
BamHI 1427 GGATCC G^GATCC
2751 GGATCC
BanI 1100 GGTACC G^G[CT][GA]CC
1714 GGCACC
BbrPI 475 CACGTG CAC^GTG
2876 CACGTG
BciVI 265 GGATAC GTATCC(6/5)
822 GTATCC
BsaI 299 GAGACC GGTCTC(1/5)
3024 GAGACC
BsiHKAI 66 GAGCTC G[AT]GC[AT]^C
2826 GAGCAC
EcoNI 252 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
255 CCTCCAGGAGG
HincII 1653 GTTGAC GT[CT]^[GA]AC
3330 GTCAAC
HindII 1653 GTTGAC GT[CT]^[GA]AC
3330 GTCAAC
MspA1I 2284 CCGCTG C[AC]G^C[GT]G
2738 CCGCTG
NcoI 1548 CCATGG C^CATGG
1879 CCATGG
PmaCI 475 CACGTG CAC^GTG
2876 CACGTG
PmlI 475 CACGTG CAC^GTG
2876 CACGTG
SphI 1129 GCATGC GCATG^C
2210 GCATGC
SspI 3406 AATATT AAT^ATT
3959 AATATT
XmnI 1940 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3378 GAACAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaJI BseRI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PshBI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII