################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII AclI AhdI AscI AseI AsnI AspEI AvrII BbrPI BbvCI BciVI BclI BlnI BsaAI BsaBI BsiWI BsmBI BspDI BspHI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI DraI DraIII Eam1105I EcoO65I EcoRV EcoT22I FbaI HincII HindII HpaI MamI MfeI MluI MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI SalI SbfI ScaI SfiI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 2103 GTCTAC GT^[AC][GT]AC AflIII 4123 ACATGT A^C[GA][CT]GT AgeI 175 ACCGGT A^CCGGT Alw44I 179 GTGCAC G^TGCAC AocI 3711 CCTCAGG CC^T[ACGT]AGG Aor51HI 433 AGCGCT AGC^GCT ApaLI 179 GTGCAC G^TGCAC Asp700I 1049 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 393 TGCGCA TGC^GCA BamHI 541 GGATCC G^GATCC BcgI 2225 CGACCAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1360 AGATCT A^GATCT BsaI 2849 GGTCTC GGTCTC(1/5) Bsp1407I 1828 TGTACA T^GTACA BspLU11I 4123 ACATGT A^CATGT BsrDI 1656 CATTGC GCAATG(2/0) BsrGI 1828 TGTACA T^GTACA BssSI 1356 CACGAG CACGAG(-5/-1) Bsu36I 3711 CCTCAGG CC^T[ACGT]AGG CpoI 20 CGGACCG CG^G[AT]CCG DrdI 918 GACTTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 433 AGCGCT AGC^GCT Eco81I 3711 CCTCAGG CC^T[ACGT]AGG EcoRI 3575 GAATTC G^AATTC FspI 393 TGCGCA TGC^GCA NheI 636 GCTAGC G^CTAGC PinAI 175 ACCGGT A^CCGGT PshAI 2843 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 20 CGGACCG CG^G[AT]CCG SacI 3229 GAGCTC GAGCT^C SapI 2093 GAAGAGC GCTCTTC(1/4) SexAI 1876 ACCTGGT A^CC[AT]GGT SgrAI 174 CACCGGTG C[GA]^CCGG[CT]G SmaI 2692 CCCGGG CCC^GGG SspBI 1828 TGTACA T^GTACA StuI 1552 AGGCCT AGG^CCT XbaI 3479 TCTAGA T^CTAGA XmaCI 2692 CCCGGG C^CCGGG XmaI 2692 CCCGGG C^CCGGG XmnI 1049 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 1165 GGTACC G^GTACC 2527 GGTACC AccIII 2344 TCCGGA T^CCGGA 2596 TCCGGA AcsI 849 GAATTT [GA]^AATT[CT] 3575 GAATTC AflII 3446 CTTAAG C^TTAAG 3846 CTTAAG ApoI 849 GAATTT [GA]^AATT[CT] 3575 GAATTC Asp718I 1165 GGTACC G^GTACC 2527 GGTACC AspI 1875 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2337 GACAGTGTC BbeI 289 GGCGCC GGCGC^C 492 GGCGCC BfrI 3446 CTTAAG C^TTAAG 3846 CTTAAG BseAI 2344 TCCGGA T^CCGGA 2596 TCCGGA BsiEI 377 CGGCCG CG[GA][CT]^CG 448 CGGCCG BsmI 1337 GAATGC GAATGC(1/-1) 316 GCATTC BspEI 2344 TCCGGA T^CCGGA 2596 TCCGGA BsrBI 2967 GAGCGG CCGCTC(-3/-3) 3109 GAGCGG BssHII 468 GCGCGC G^CGCGC 1861 GCGCGC EagI 377 CGGCCG C^GGCCG 448 CGGCCG EclXI 377 CGGCCG C^GGCCG 448 CGGCCG Eco52I 377 CGGCCG C^GGCCG 448 CGGCCG EcoNI 2363 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3073 CCTCCAGGAGG FseI 378 GGCCGGCC GGCCGG^CC 2789 GGCCGGCC KasI 289 GGCGCC G^GCGCC 492 GGCGCC KpnI 1165 GGTACC GGTAC^C 2527 GGTACC KspI 451 CCGCGG CCGC^GG 2801 CCGCGG MaeII 1123 ACGT A^CGT 2101 ACGT MroI 2344 TCCGGA T^CCGGA 2596 TCCGGA NarI 289 GGCGCC GG^CGCC 492 GGCGCC PflFI 1875 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2337 GACAGTGTC PpuMI 2575 GGGACCC [GA]G^G[AT]CC[CT] 3058 AGGACCT PvuII 3000 CAGCTG CAG^CTG 3551 CAGCTG SacII 451 CCGCGG CCGC^GG 2801 CCGCGG SfoI 289 GGCGCC GGC^GCC 492 GGCGCC Tth111I 1875 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2337 GACAGTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII