##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AhdI AscI AseI
AsnI AspEI AvrII BbrPI BbvCI
BciVI BclI BlnI BsaAI BsaBI
BsiWI BsmBI BspDI BspHI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI DraI DraIII Eam1105I EcoO65I
EcoRV EcoT22I FbaI HincII HindII
HpaI MamI MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI SalI
SbfI ScaI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 2103 GTCTAC GT^[AC][GT]AC
AflIII 4123 ACATGT A^C[GA][CT]GT
AgeI 175 ACCGGT A^CCGGT
Alw44I 179 GTGCAC G^TGCAC
AocI 3711 CCTCAGG CC^T[ACGT]AGG
Aor51HI 433 AGCGCT AGC^GCT
ApaLI 179 GTGCAC G^TGCAC
Asp700I 1049 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 393 TGCGCA TGC^GCA
BamHI 541 GGATCC G^GATCC
BcgI 2225 CGACCAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1360 AGATCT A^GATCT
BsaI 2849 GGTCTC GGTCTC(1/5)
Bsp1407I 1828 TGTACA T^GTACA
BspLU11I 4123 ACATGT A^CATGT
BsrDI 1656 CATTGC GCAATG(2/0)
BsrGI 1828 TGTACA T^GTACA
BssSI 1356 CACGAG CACGAG(-5/-1)
Bsu36I 3711 CCTCAGG CC^T[ACGT]AGG
CpoI 20 CGGACCG CG^G[AT]CCG
DrdI 918 GACTTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 433 AGCGCT AGC^GCT
Eco81I 3711 CCTCAGG CC^T[ACGT]AGG
EcoRI 3575 GAATTC G^AATTC
FspI 393 TGCGCA TGC^GCA
NheI 636 GCTAGC G^CTAGC
PinAI 175 ACCGGT A^CCGGT
PshAI 2843 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 20 CGGACCG CG^G[AT]CCG
SacI 3229 GAGCTC GAGCT^C
SapI 2093 GAAGAGC GCTCTTC(1/4)
SexAI 1876 ACCTGGT A^CC[AT]GGT
SgrAI 174 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2692 CCCGGG CCC^GGG
SspBI 1828 TGTACA T^GTACA
StuI 1552 AGGCCT AGG^CCT
XbaI 3479 TCTAGA T^CTAGA
XmaCI 2692 CCCGGG C^CCGGG
XmaI 2692 CCCGGG C^CCGGG
XmnI 1049 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1165 GGTACC G^GTACC
2527 GGTACC
AccIII 2344 TCCGGA T^CCGGA
2596 TCCGGA
AcsI 849 GAATTT [GA]^AATT[CT]
3575 GAATTC
AflII 3446 CTTAAG C^TTAAG
3846 CTTAAG
ApoI 849 GAATTT [GA]^AATT[CT]
3575 GAATTC
Asp718I 1165 GGTACC G^GTACC
2527 GGTACC
AspI 1875 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2337 GACAGTGTC
BbeI 289 GGCGCC GGCGC^C
492 GGCGCC
BfrI 3446 CTTAAG C^TTAAG
3846 CTTAAG
BseAI 2344 TCCGGA T^CCGGA
2596 TCCGGA
BsiEI 377 CGGCCG CG[GA][CT]^CG
448 CGGCCG
BsmI 1337 GAATGC GAATGC(1/-1)
316 GCATTC
BspEI 2344 TCCGGA T^CCGGA
2596 TCCGGA
BsrBI 2967 GAGCGG CCGCTC(-3/-3)
3109 GAGCGG
BssHII 468 GCGCGC G^CGCGC
1861 GCGCGC
EagI 377 CGGCCG C^GGCCG
448 CGGCCG
EclXI 377 CGGCCG C^GGCCG
448 CGGCCG
Eco52I 377 CGGCCG C^GGCCG
448 CGGCCG
EcoNI 2363 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3073 CCTCCAGGAGG
FseI 378 GGCCGGCC GGCCGG^CC
2789 GGCCGGCC
KasI 289 GGCGCC G^GCGCC
492 GGCGCC
KpnI 1165 GGTACC GGTAC^C
2527 GGTACC
KspI 451 CCGCGG CCGC^GG
2801 CCGCGG
MaeII 1123 ACGT A^CGT
2101 ACGT
MroI 2344 TCCGGA T^CCGGA
2596 TCCGGA
NarI 289 GGCGCC GG^CGCC
492 GGCGCC
PflFI 1875 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2337 GACAGTGTC
PpuMI 2575 GGGACCC [GA]G^G[AT]CC[CT]
3058 AGGACCT
PvuII 3000 CAGCTG CAG^CTG
3551 CAGCTG
SacII 451 CCGCGG CCGC^GG
2801 CCGCGG
SfoI 289 GGCGCC GGC^GCC
492 GGCGCC
Tth111I 1875 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2337 GACAGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII