##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AspHI AviII BbrPI
BbvCI BcgI BglII BsaAI BsaWI
BseAI BsiEI BsiHKAI BsiWI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoT22I FseI FspI HindIII
KpnI KspI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI SfiI SfuI SgrAI SnaBI
SphI Sse8387I SspBI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflIII 201 ACATGT A^C[GA][CT]GT
AlwNI 1849 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2009 CCTCAGG CC^T[ACGT]AGG
AseI 2618 ATTAAT AT^TAAT
AsnI 2618 ATTAAT AT^TAAT
AspI 528 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2405 CCTAGG C^CTAGG
BamHI 2733 GGATCC G^GATCC
BglI 384 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2405 CCTAGG C^CTAGG
BlpI 1198 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1198 GCTCAGC GC^T[ACGT]AGC
BsaBI 149 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 402 GAGACG CGTCTC(1/5)
BsmI 1188 GAATGC GAATGC(1/-1)
BspLU11I 201 ACATGT A^CATGT
BstAPI 282 GCACAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2350 GGTTACC G^GT[ACGT]ACC
BstPI 2350 GGTTACC G^GT[ACGT]ACC
Bsu36I 2009 CCTCAGG CC^T[ACGT]AGG
CelII 1198 GCTCAGC GC^T[ACGT]AGC
EarI 1221 CTCTTC CTCTTC(1/4)
Eco81I 2009 CCTCAGG CC^T[ACGT]AGG
EcoNI 292 CCTTCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2350 GGTTACC G^GT[ACGT]ACC
EcoRI 3371 GAATTC G^AATTC
EcoRV 2296 GATATC GAT^ATC
HpaI 1883 GTTAAC GTT^AAC
Ksp632I 1221 CTCTTC CTCTTC(1/4)
MamI 149 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 528 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2661 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2663 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2618 ATTAAT AT^TAAT
PstI 3386 CTGCAG CTGCA^G
PvuII 354 CAGCTG CAG^CTG
SapI 1220 GCTCTTC GCTCTTC(1/4)
ScaI 2852 AGTACT AGT^ACT
SexAI 364 ACCTGGT A^CC[AT]GGT
SmaI 1679 CCCGGG CCC^GGG
SpeI 2849 ACTAGT A^CTAGT
SspI 2339 AATATT AAT^ATT
StuI 3363 AGGCCT AGG^CCT
Tth111I 528 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1679 CCCGGG C^CCGGG
XmaI 1679 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 1026 GTCTAC GT^[AC][GT]AC
2305 GTCTAC
AflII 2328 CTTAAG C^TTAAG
2879 CTTAAG
ApaI 759 GGGCCC GGGCC^C
2922 GGGCCC
Asp700I 2078 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2779 GAAAGTGTTC
BalI 52 TGGCCA TGG^CCA
1822 TGGCCA
BciVI 410 GGATAC GTATCC(6/5)
2829 GGATAC
BclI 706 TGATCA T^GATCA
1121 TGATCA
BfrI 2328 CTTAAG C^TTAAG
2879 CTTAAG
BsaI 1081 GAGACC GGTCTC(1/5)
2004 GGTCTC
BsgI 280 CTGCAC GTGCAG(16/14)
348 CTGCAC
FbaI 706 TGATCA T^GATCA
1121 TGATCA
HincII 1883 GTTAAC GT[CT]^[GA]AC
2208 GTTGAC
HindII 1883 GTTAAC GT[CT]^[GA]AC
2208 GTTGAC
MluNI 52 TGGCCA TGG^CCA
1822 TGGCCA
MscI 52 TGGCCA TGG^CCA
1822 TGGCCA
MspA1I 354 CAGCTG C[AC]G^C[GT]G
2000 CAGCGG
NaeI 101 GCCGGC GCC^GGC
316 GCCGGC
NgoMIV 101 GCCGGC G^CCGGC
316 GCCGGC
XmnI 2078 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2779 GAAAGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AvaI AvaII
BanI BanII BbeI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII