##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccIII AflII AgeI ApaI
AscI Asp718I AvrII BciVI BfrI
BglI BlnI BsaBI BseAI BsiEI
BsiWI Bsp1407I BspEI BspHI BspMI
BsrBI BsrFI BsrGI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Cfr10I
CpoI DraIII EagI EclXI Eco52I
EcoNI EcoO65I EcoRI FauI FseI
HindIII KpnI KspI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PinAI PmeI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AclI 1333 AACGTT AA^CGTT
AhdI 2417 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1278 GTGCAC G^TGCAC
AocI 415 CCTGAGG CC^T[ACGT]AGG
ApaLI 1278 GTGCAC G^TGCAC
Asp700I 551 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2417 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1278 GTGCAC G[AT]GC[AT]^C
AspI 1922 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 979 CTCGAG C^[CT]CG[GA]G
AviII 3045 TGCGCA TGC^GCA
BalI 597 TGGCCA TGG^CCA
BbeI 92 GGCGCC GGCGC^C
BbrPI 2318 CACGTG CAC^GTG
BclI 1393 TGATCA T^GATCA
BlpI 1981 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1981 GCTCAGC GC^T[ACGT]AGC
BsaAI 2318 CACGTG [CT]AC^GT[GA]
BsaI 10 GGTCTC GGTCTC(1/5)
BsaWI 1287 ACCGGA [AT]^CCGG[AT]
BseRI 1316 GAGGAG GAGGAG(10/8)
BsiHKAI 1278 GTGCAC G[AT]GC[AT]^C
BsmBI 1507 CGTCTC CGTCTC(1/5)
BsmI 2206 GAATGC GAATGC(1/-1)
BsoBI 979 CTCGAG C^[CT]CG[GA]G
BspDI 285 ATCGAT AT^CGAT
BspLU11I 1953 ACATGT A^CATGT
BssHII 116 GCGCGC G^CGCGC
BssSI 2171 CACGAG CACGAG(-5/-1)
Bsu36I 415 CCTGAGG CC^T[ACGT]AGG
CelII 1981 GCTCAGC GC^T[ACGT]AGC
ClaI 285 ATCGAT AT^CGAT
Eam1105I 2417 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 415 CCTGAGG CC^T[ACGT]AGG
EcoRV 2661 GATATC GAT^ATC
FbaI 1393 TGATCA T^GATCA
FspI 3045 TGCGCA TGC^GCA
HpaI 1670 GTTAAC GTT^AAC
KasI 92 GGCGCC G^GCGCC
MluNI 597 TGGCCA TGG^CCA
MscI 597 TGGCCA TGG^CCA
NarI 92 GGCGCC GG^CGCC
PacI 327 TTAATTAA TTAAT^TAA
PaeR7I 979 CTCGAG C^TCGAG
PflFI 1922 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2318 CACGTG CAC^GTG
PmlI 2318 CACGTG CAC^GTG
PshAI 1182 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1333 AACGTT AA^CGTT
SfoI 92 GGCGCC GGC^GCC
SspI 3075 AATATT AAT^ATT
Tth111I 1922 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 979 CTCGAG C^TCGAG
XmnI 551 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 617 GACGTC GACGT^C
2540 GACGTC
AccI 2345 GTAGAC GT^[AC][GT]AC
3436 GTCTAC
AflIII 1953 ACATGT A^C[GA][CT]GT
2625 ACGTGT
Aor51HI 2215 AGCGCT AGC^GCT
2580 AGCGCT
BamHI 790 GGATCC G^GATCC
1290 GGATCC
BanI 92 GGCGCC G^G[CT][GA]CC
1303 GGCACC
BbvCI 1053 CCTCAGC CCTCAGC(-5/-2)
253 GCTGAGG
BcgI 435 CGAGGCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2608 CGAGCCTAATGC
DraI 1687 TTTAAA TTT^AAA
3562 TTTAAA
DrdI 1330 GACAACGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2348 GACAAACCAGTC
Eco47III 2215 AGCGCT AGC^GCT
2580 AGCGCT
EcoT22I 1719 ATGCAT ATGCA^T
3587 ATGCAT
HgaI 530 GACGC GACGC(5/10)
3151 GCGTC
NsiI 1719 ATGCAT ATGCA^T
3587 ATGCAT
PflMI 123 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1294 CCAGAACCTGG
ScaI 553 AGTACT AGT^ACT
2325 AGTACT
SmlI 13 CTCAAG C^T[CT][GA]AG
979 CTCGAG
SphI 170 GCATGC GCATG^C
1657 GCATGC
StuI 437 AGGCCT AGG^CCT
1131 AGGCCT
Van91I 123 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1294 CCAGAACCTGG
XcmI 166 CCAAGCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
828 CCAACATCAAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI AvaII BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaHI BsaJI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII