##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AccIII AclI AflIII AgeI Alw44I
Aor51HI ApaLI AscI AspI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BspHI BspLU11I BsrBI BssHII BstBI
BstEII BstPI BstXI CelII ClaI
CpoI DraIII DrdI Eco47III EcoO65I
EcoT22I FseI FspI HpaI KspI
MamI MfeI MluI MroI MunI
NdeI NheI NruI NsiI NspV
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfuI
SpeI Sse8387I SspI SwaI Tth111I
Van91I XcmI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 314 GACGTC GACGT^C
Acc65I 603 GGTACC G^GTACC
AhdI 3143 GACCTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 581 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2950 CCTCAGG CC^T[ACGT]AGG
ApaI 3443 GGGCCC GGGCC^C
Asp718I 603 GGTACC G^GTACC
AspEI 3143 GACCTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1275 GTGCTC G[AT]GC[AT]^C
BalI 1431 TGGCCA TGG^CCA
BclI 3288 TGATCA T^GATCA
BglI 866 GCCTTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3364 AGATCT A^GATCT
BsaAI 3620 TACGTA [CT]AC^GT[GA]
BsaI 683 GAGACC GGTCTC(1/5)
BsgI 710 GTGCAG GTGCAG(16/14)
BsiHKAI 1275 GTGCTC G[AT]GC[AT]^C
BsmBI 725 GAGACG CGTCTC(1/5)
BsmI 1510 GAATGC GAATGC(1/-1)
BssSI 722 CACGAG CACGAG(-5/-1)
BstAPI 1058 GCAGGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2950 CCTCAGG CC^T[ACGT]AGG
Eam1105I 3143 GACCTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2950 CCTCAGG CC^T[ACGT]AGG
EcoRV 1752 GATATC GAT^ATC
FbaI 3288 TGATCA T^GATCA
HincII 1125 GTTGAC GT[CT]^[GA]AC
HindII 1125 GTTGAC GT[CT]^[GA]AC
KpnI 603 GGTACC GGTAC^C
MluNI 1431 TGGCCA TGG^CCA
MscI 1431 TGGCCA TGG^CCA
MslI 3420 CATAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI 283 GCGGCCGC GC^GGCCGC
PacI 3351 TTAATTAA TTAAT^TAA
PaeR7I 792 CTCGAG C^TCGAG
ScaI 1566 AGTACT AGT^ACT
SmaI 142 CCCGGG CCC^GGG
SnaBI 3620 TACGTA TAC^GTA
SphI 741 GCATGC GCATG^C
StuI 174 AGGCCT AGG^CCT
XbaI 1950 TCTAGA T^CTAGA
XhoI 792 CTCGAG C^TCGAG
XmaCI 142 CCCGGG C^CCGGG
XmaI 142 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 1612 GTATAC GT^[AC][GT]AC
1958 GTATAC
AflII 1765 CTTAAG C^TTAAG
3360 CTTAAG
Asp700I 1580 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2699 GAACTCTTTC
BfrI 1765 CTTAAG C^TTAAG
3360 CTTAAG
Bsp1407I 1571 TGTACA T^GTACA
3756 TGTACA
BsrDI 50 GCAATG GCAATG(2/0)
2932 GCAATG
BsrGI 1571 TGTACA T^GTACA
3756 TGTACA
Bst1107I 1612 GTATAC GTA^TAC
1958 GTATAC
BstYI 3364 AGATCT [GA]^GATC[CT]
3491 GGATCT
BstZ17I 1612 GTATAC GTA^TAC
1958 GTATAC
DsaI 2435 CCATGG C^C[GA][CT]GG
2832 CCATGG
EcoNI 2063 CCTTAGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3638 CCTCCAAAAGG
EcoRI 1471 GAATTC G^AATTC
2267 GAATTC
HgaI 401 GACGC GACGC(5/10)
538 GACGC
MflI 3364 AGATCT [GA]^GATC[CT]
3491 GGATCT
NcoI 2435 CCATGG C^CATGG
2832 CCATGG
NspI 741 GCATGC [GA]CATG^[CT]
3733 ACATGC
PvuII 2309 CAGCTG CAG^CTG
3797 CAGCTG
SgrAI 232 CGCCGGCG C[GA]^CCGG[CT]G
622 CACCGGCG
SspBI 1571 TGTACA T^GTACA
3756 TGTACA
XhoII 3364 AGATCT [GA]^GATC[CT]
3491 GGATCT
XmnI 1580 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2699 GAACTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AseI AsnI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I