##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI BbrPI BbsI BfrI BglII
BpuAI BsaBI BsiWI Bsp1407I BspDI
BspHI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI Eco47III
EcoRI EcoRV EcoT22I FseI HindIII
HpaI MamI MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 2195 GGTACC G^GTACC
AocI 1216 CCTGAGG CC^T[ACGT]AGG
Asp718I 2195 GGTACC G^GTACC
AspI 1134 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3138 CCTAGG C^CTAGG
BclI 2681 TGATCA T^GATCA
BlnI 3138 CCTAGG C^CTAGG
BsaAI 2341 TACGTG [CT]AC^GT[GA]
BsmI 1857 GAATGC GAATGC(1/-1)
BspLU11I 2354 ACATGT A^CATGT
BstEII 225 GGTGACC G^GT[ACGT]ACC
BstPI 225 GGTGACC G^GT[ACGT]ACC
Bsu36I 1216 CCTGAGG CC^T[ACGT]AGG
DraI 3927 TTTAAA TTT^AAA
DraIII 1070 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 417 GACGGTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1216 CCTGAGG CC^T[ACGT]AGG
EcoO65I 225 GGTGACC G^GT[ACGT]ACC
FbaI 2681 TGATCA T^GATCA
KpnI 2195 GGTACC GGTAC^C
KspI 13 CCGCGG CCGC^GG
NheI 4051 GCTAGC G^CTAGC
PaeR7I 4046 CTCGAG C^TCGAG
PflFI 1134 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 488 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 13 CCGCGG CCGC^GG
ScaI 1385 AGTACT AGT^ACT
SexAI 221 ACCTGGT A^CC[AT]GGT
SfiI 3612 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 1656 AGGCCT AGG^CCT
TfiI 676 GAATC G^A[AT]TC
Tth111I 1134 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 488 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3910 TCTAGA T^CTAGA
XhoI 4046 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccIII 2557 TCCGGA T^CCGGA
3002 TCCGGA
AflIII 1196 ACGTGT A^C[GA][CT]GT
2354 ACATGT
AviII 563 TGCGCA TGC^GCA
3035 TGCGCA
BamHI 611 GGATCC G^GATCC
2531 GGATCC
BcgI 1590 CGAGAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1851 CGAGGTGAATGC
BciVI 2560 GGATAC GTATCC(6/5)
1490 GTATCC
BlpI 2034 GCTCAGC GC^T[ACGT]AGC
3815 GCTGAGC
Bpu1102I 2034 GCTCAGC GC^T[ACGT]AGC
3815 GCTGAGC
BseAI 2557 TCCGGA T^CCGGA
3002 TCCGGA
BsgI 472 GTGCAG GTGCAG(16/14)
3220 GTGCAG
BspEI 2557 TCCGGA T^CCGGA
3002 TCCGGA
BstAPI 3222 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3570 GCAGGTCCTGC
CelII 2034 GCTCAGC GC^T[ACGT]AGC
3815 GCTGAGC
FspI 563 TGCGCA TGC^GCA
3035 TGCGCA
HincII 1885 GTCAAC GT[CT]^[GA]AC
3502 GTCAAC
HindII 1885 GTCAAC GT[CT]^[GA]AC
3502 GTCAAC
MroI 2557 TCCGGA T^CCGGA
3002 TCCGGA
NspI 2081 ACATGC [GA]CATG^[CT]
2354 ACATGT
SapI 2437 GAAGAGC GCTCTTC(1/4)
2091 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI ApaI
ApoI Asp700I AspEI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI XmnI