##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbeI BbrPI BciVI BfrI
BlnI BsaBI BsaHI BsiWI BspDI
BspLU11I BssHII BstBI BstEII BstPI
ClaI CpoI EcoNI EcoO65I EcoRV
FseI FspI Hin1I HpaI KasI
KpnI MamI MluI NaeI NarI
NdeI NgoMIV NheI NruI NspV
PacI PflFI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SalI
SbfI SfiI SfoI SfuI SmaI
SnaBI SphI Sse8387I SspI Tth111I
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 3693 TCCGGA T^CCGGA
AgeI 325 ACCGGT A^CCGGT
AhdI 314 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3582 AGCGCT AGC^GCT
ApaI 518 GGGCCC GGGCC^C
AspEI 314 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 1022 CCTCAGC CCTCAGC(-5/-2)
BcgI 4713 CGATTCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3339 AGATCT A^GATCT
BsaAI 3596 TACGTG [CT]AC^GT[GA]
BseAI 3693 TCCGGA T^CCGGA
BspEI 3693 TCCGGA T^CCGGA
BspHI 744 TCATGA T^CATGA
BsrBI 241 GAGCGG CCGCTC(-3/-3)
BssSI 1782 CTCGTG CACGAG(-5/-1)
Bst1107I 2939 GTATAC GTA^TAC
BstAPI 385 GCACCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 882 CCAATGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2939 GTATAC GTA^TAC
Eam1105I 314 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3582 AGCGCT AGC^GCT
HaeII 3582 AGCGCT [GA]GCGC^[CT]
KspI 8 CCGCGG CCGC^GG
MfeI 2373 CAATTG C^AATTG
MroI 3693 TCCGGA T^CCGGA
MunI 2373 CAATTG C^AATTG
NotI 4 GCGGCCGC GC^GGCCGC
PaeR7I 4624 CTCGAG C^TCGAG
PflMI 3984 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 325 ACCGGT A^CCGGT
PshAI 4427 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 744 TCATGA T^CATGA
SacII 8 CCGCGG CCGC^GG
SapI 1712 GCTCTTC GCTCTTC(1/4)
ScaI 459 AGTACT AGT^ACT
SgrAI 324 CACCGGTG C[GA]^CCGG[CT]G
StuI 3927 AGGCCT AGG^CCT
SwaI 754 ATTTAAAT ATTT^AAAT
Van91I 3984 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 4624 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaI 187 CTCGGG C^[CT]CG[GA]G
4624 CTCGAG
BalI 910 TGGCCA TGG^CCA
4238 TGGCCA
BamHI 351 GGATCC G^GATCC
3690 GGATCC
BsiEI 5 CGGCCG CG[GA][CT]^CG
244 CGGCCG
BsoBI 187 CTCGGG C^[CT]CG[GA]G
4624 CTCGAG
BsrFI 325 ACCGGT [GA]^CCGG[CT]
1129 GCCGGT
Cfr10I 325 ACCGGT [GA]^CCGG[CT]
1129 GCCGGT
DrdI 433 GACCATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2513 GACACCAAGGTC
EagI 5 CGGCCG C^GGCCG
244 CGGCCG
EclXI 5 CGGCCG C^GGCCG
244 CGGCCG
Eco52I 5 CGGCCG C^GGCCG
244 CGGCCG
HgaI 346 GCGTC GACGC(5/10)
3907 GCGTC
HindIII 2210 AAGCTT A^AGCTT
3488 AAGCTT
MluNI 910 TGGCCA TGG^CCA
4238 TGGCCA
MscI 910 TGGCCA TGG^CCA
4238 TGGCCA
NspI 3955 GCATGT [GA]CATG^[CT]
4392 ACATGC
PpuMI 2520 AGGTCCT [GA]G^G[AT]CC[CT]
4661 GGGACCT
SacI 204 GAGCTC GAGCT^C
502 GAGCTC
SexAI 480 ACCAGGT A^CC[AT]GGT
687 ACCAGGT
SmlI 3418 CTCAAG C^T[CT][GA]AG
4624 CTCGAG
SpeI 1801 ACTAGT A^CTAGT
4821 ACTAGT
XcmI 2241 CCAAATTTCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3759 CCATGGAGAATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BanI BanII BbsI
BbvI BclI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI Eco81I EcoO109I EcoRI EcoRII
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NsiI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII