##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AvaI AviII BamHI BbeI BbrPI
BcnI BfrI BlpI Bpu1102I BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsoBI BspDI BspEI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I EcoO65I
FseI FspI HaeII HapII Hin1I
HindIII HpaI HpaII KasI KpnI
KspI MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AhdI 1386 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 588 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3915 CCTCAGG CC^T[ACGT]AGG
AspEI 1386 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 28 GGTCC G^G[AT]CC
BanI 2931 GGTGCC G^G[CT][GA]CC
BbsI 997 GTCTTC GAAGAC(2/6)
BbvCI 3798 CCTCAGC CCTCAGC(-5/-2)
BclI 1361 TGATCA T^GATCA
BglII 1233 AGATCT A^GATCT
BpuAI 997 GTCTTC GAAGAC(2/6)
BsaAI 2448 TACGTA [CT]AC^GT[GA]
BsaBI 780 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI 1 GAGCGG CCGCTC(-3/-3)
Bsu36I 3915 CCTCAGG CC^T[ACGT]AGG
CfoI 3832 GCGC GCG^C
DraII 27 GGGTCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1386 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3915 CCTCAGG CC^T[ACGT]AGG
EcoNI 4046 CCTAAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 27 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 284 GATATC GAT^ATC
EcoT22I 2752 ATGCAT ATGCA^T
FauI 18 GCGGG CCCGC(4/6)
FbaI 1361 TGATCA T^GATCA
HgaI 206 GCGTC GACGC(5/10)
HhaI 3832 GCGC GCG^C
HinP1I 3832 GCGC G^CGC
MamI 780 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 2752 ATGCAT ATGCA^T
PpuMI 27 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 111 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 193 CAGCTG CAG^CTG
SacI 3301 GAGCTC GAGCT^C
SapI 4549 GAAGAGC GCTCTTC(1/4)
ScaI 3818 AGTACT AGT^ACT
SnaBI 2448 TACGTA TAC^GTA
SpeI 492 ACTAGT A^CTAGT
SphI 3828 GCATGC GCATG^C
XcmI 930 CCATTTCAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AspHI 124 GTGCTC G[AT]GC[AT]^C
3301 GAGCTC
AvrII 728 CCTAGG C^CTAGG
844 CCTAGG
BalI 2554 TGGCCA TGG^CCA
3889 TGGCCA
BcgI 550 CGATACCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2189 GCAATCCTCTCG
BlnI 728 CCTAGG C^CTAGG
844 CCTAGG
BsiHKAI 124 GTGCTC G[AT]GC[AT]^C
3301 GAGCTC
BsmFI 4448 GGGAC GGGAC(10/14)
2298 GTCCC
BsmI 2750 GAATGC GAATGC(1/-1)
1417 GCATTC
Bsp1407I 60 TGTACA T^GTACA
1980 TGTACA
BspHI 3008 TCATGA T^CATGA
3297 TCATGA
BsrDI 1271 CATTGC GCAATG(2/0)
992 GCAATG
BsrGI 60 TGTACA T^GTACA
1980 TGTACA
BstF5I 2746 GGATG GGATG(2/0)
2915 GGATG
BstXI 892 CCAGAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3943 CCAAAGTGTTGG
EaeI 2554 TGGCCA [CT]^GGCC[GA]
3889 TGGCCA
FokI 2746 GGATG GGATG(9/13)
2915 GGATG
HincII 1383 GTTGAC GT[CT]^[GA]AC
2487 GTCAAC
HindII 1383 GTTGAC GT[CT]^[GA]AC
2487 GTCAAC
MluNI 2554 TGGCCA TGG^CCA
3889 TGGCCA
MscI 2554 TGGCCA TGG^CCA
3889 TGGCCA
MspA1I 16 CAGCGG C[AC]G^C[GT]G
193 CAGCTG
NdeI 2285 CATATG CA^TATG
3414 CATATG
NlaIV 27 GGGTCC GG[ACGT]^[ACGT]CC
2931 GGTGCC
PflMI 1127 CCAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2281 CCAGCATATGG
PstI 713 CTGCAG CTGCA^G
833 CTGCAG
RcaI 3008 TCATGA T^CATGA
3297 TCATGA
SspBI 60 TGTACA T^GTACA
1980 TGTACA
StuI 1592 AGGCCT AGG^CCT
3307 AGGCCT
TaqI 119 TCGA T^CGA
2171 TCGA
TthHB8I 119 TCGA T^CGA
2171 TCGA
Van91I 1127 CCAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2281 CCAGCATATGG
XbaI 1602 TCTAGA T^CTAGA
2601 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
AspI BanII BbvI BciVI BfaI
BglI BmyI BpmI BsaI BsaJI
BsgI BsiYI BslI BsmAI BsmBI
Bsp1286I BspLU11I BspMI BsrI BssKI
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRI EcoRII
EcoT14I Fnu4HI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NspI PflFI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
XhoII XmnI