##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AclI AflII Aor51HI AscI
AseI AsnI Asp718I AspI AvrII
BfrI BlnI BlpI Bpu1102I BsaBI
BsiWI BspHI BssHII Bst1107I BstBI
BstZ17I CelII CpoI DrdI Eco47III
EcoNI EcoRV FseI HindIII HpaI
KpnI KspI MamI MluI NdeI
NheI NruI NspV PacI PaeR7I
PflFI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 703 GACGTC GACGT^C
AflIII 1513 ACATGT A^C[GA][CT]GT
AgeI 1982 ACCGGT A^CCGGT
AhdI 182 GACTAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1920 GTGCAC G^TGCAC
ApaLI 1920 GTGCAC G^TGCAC
Asp700I 3471 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 182 GACTAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 299 GGCGCC GGCGC^C
BbrPI 580 CACGTG CAC^GTG
BglI 2803 GCCAACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1781 AGATCT A^GATCT
BsaAI 580 CACGTG [CT]AC^GT[GA]
Bsp1407I 665 TGTACA T^GTACA
BspDI 2989 ATCGAT AT^CGAT
BspLU11I 1513 ACATGT A^CATGT
BsrGI 665 TGTACA T^GTACA
BstEII 1658 GGTCACC G^GT[ACGT]ACC
BstPI 1658 GGTCACC G^GT[ACGT]ACC
ClaI 2989 ATCGAT AT^CGAT
DraI 1459 TTTAAA TTT^AAA
Eam1105I 182 GACTAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1658 GGTCACC G^GT[ACGT]ACC
EcoRI 1358 GAATTC G^AATTC
EcoT22I 202 ATGCAT ATGCA^T
HaeII 299 GGCGCC [GA]GCGC^[CT]
KasI 299 GGCGCC G^GCGCC
NaeI 1837 GCCGGC GCC^GGC
NarI 299 GGCGCC GG^CGCC
NgoMIV 1837 GCCGGC G^CCGGC
NotI 1370 GCGGCCGC GC^GGCCGC
NsiI 202 ATGCAT ATGCA^T
PinAI 1982 ACCGGT A^CCGGT
PmaCI 580 CACGTG CAC^GTG
PmlI 580 CACGTG CAC^GTG
SapI 624 GAAGAGC GCTCTTC(1/4)
SfoI 299 GGCGCC GGC^GCC
SgrAI 1981 CACCGGTG C[GA]^CCGG[CT]G
SmaI 464 CCCGGG CCC^GGG
SspBI 665 TGTACA T^GTACA
XmaCI 464 CCCGGG C^CCGGG
XmaI 464 CCCGGG C^CCGGG
XmnI 3471 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 2611 GTCTAC GT^[AC][GT]AC
3602 GTAGAC
AccIII 52 TCCGGA T^CCGGA
2688 TCCGGA
AcsI 1358 GAATTC [GA]^AATT[CT]
2402 AAATTC
AocI 444 CCTGAGG CC^T[ACGT]AGG
843 CCTCAGG
ApaI 315 GGGCCC GGGCC^C
656 GGGCCC
ApoI 1358 GAATTC [GA]^AATT[CT]
2402 AAATTC
AvaI 464 CCCGGG C^[CT]CG[GA]G
3339 CTCGGG
AviII 1025 TGCGCA TGC^GCA
3434 TGCGCA
BamHI 136 GGATCC G^GATCC
1538 GGATCC
BbvCI 552 CCTCAGC CCTCAGC(-5/-2)
1098 GCTGAGG
BcgI 3222 CGATGACTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3447 GCATGACCCTCG
BciVI 3334 GGATAC GTATCC(6/5)
2579 GTATCC
BclI 1529 TGATCA T^GATCA
3703 TGATCA
BseAI 52 TCCGGA T^CCGGA
2688 TCCGGA
BsiEI 111 CGGCCG CG[GA][CT]^CG
1371 CGGCCG
BsoBI 464 CCCGGG C^[CT]CG[GA]G
3339 CTCGGG
BspEI 52 TCCGGA T^CCGGA
2688 TCCGGA
BsrDI 1352 GCAATG GCAATG(2/0)
2216 GCAATG
BssSI 3321 CACGAG CACGAG(-5/-1)
2899 CTCGTG
BstXI 164 CCATGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
728 CCAGAGACCTGG
Bsu36I 444 CCTGAGG CC^T[ACGT]AGG
843 CCTCAGG
EagI 111 CGGCCG C^GGCCG
1371 CGGCCG
EarI 624 GAAGAG CTCTTC(1/4)
1072 GAAGAG
EclXI 111 CGGCCG C^GGCCG
1371 CGGCCG
Eco52I 111 CGGCCG C^GGCCG
1371 CGGCCG
Eco81I 444 CCTGAGG CC^T[ACGT]AGG
843 CCTCAGG
FbaI 1529 TGATCA T^GATCA
3703 TGATCA
FspI 1025 TGCGCA TGC^GCA
3434 TGCGCA
HincII 3013 GTTGAC GT[CT]^[GA]AC
3133 GTCAAC
HindII 3013 GTTGAC GT[CT]^[GA]AC
3133 GTCAAC
Ksp632I 624 GAAGAG CTCTTC(1/4)
1072 GAAGAG
MfeI 1805 CAATTG C^AATTG
2667 CAATTG
MroI 52 TCCGGA T^CCGGA
2688 TCCGGA
MseI 1460 TTAA T^TAA
3173 TTAA
MunI 1805 CAATTG C^AATTG
2667 CAATTG
SexAI 395 ACCTGGT A^CC[AT]GGT
2270 ACCTGGT
TfiI 3041 GATTC G^A[AT]TC
3051 GATTC
Tru9I 1460 TTAA T^TAA
3173 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII