##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AgeI Aor51HI AscI Asp718I
AspI AvrII BbeI BbrPI BbvCI
BcgI BlnI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BspMI BsrBI BsrDI
BssHII Bst1107I BstBI BstUI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoRV FauI
FseI HaeII KasI KpnI KspI
MluI MroI MvnI NarI NdeI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 1862 GTCTAC GT^[AC][GT]AC
AflII 2697 CTTAAG C^TTAAG
AhdI 707 GACGAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1181 GTGCAC G^TGCAC
AlwNI 3844 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1532 CCTAAGG CC^T[ACGT]AGG
ApaLI 1181 GTGCAC G^TGCAC
AspEI 707 GACGAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1497 TGCGCA TGC^GCA
BalI 492 TGGCCA TGG^CCA
BamHI 195 GGATCC G^GATCC
BclI 1616 TGATCA T^GATCA
BcnI 156 CCCGG CC^[GC]GG
BfrI 2697 CTTAAG C^TTAAG
BglI 292 GCCGATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2141 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 3443 TGTACA T^GTACA
BsrFI 331 GCCGGC [GA]^CCGG[CT]
BsrGI 3443 TGTACA T^GTACA
BssSI 3113 CTCGTG CACGAG(-5/-1)
BstAPI 338 GCATTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 36 GGTAACC G^GT[ACGT]ACC
BstPI 36 GGTAACC G^GT[ACGT]ACC
Bsu36I 1532 CCTAAGG CC^T[ACGT]AGG
CfoI 1498 GCGC GCG^C
Cfr10I 331 GCCGGC [GA]^CCGG[CT]
DraIII 3120 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 695 CCATGG C^C[GA][CT]GG
EaeI 492 TGGCCA [CT]^GGCC[GA]
Eam1105I 707 GACGAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1532 CCTAAGG CC^T[ACGT]AGG
EcoO65I 36 GGTAACC G^GT[ACGT]ACC
EcoRI 943 GAATTC G^AATTC
EcoT22I 1903 ATGCAT ATGCA^T
FbaI 1616 TGATCA T^GATCA
FspI 1497 TGCGCA TGC^GCA
HhaI 1498 GCGC GCG^C
HinP1I 1498 GCGC G^CGC
HpaI 3065 GTTAAC GTT^AAC
MamI 2141 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 557 CAATTG C^AATTG
MluNI 492 TGGCCA TGG^CCA
MscI 492 TGGCCA TGG^CCA
MunI 557 CAATTG C^AATTG
NaeI 331 GCCGGC GCC^GGC
NciI 156 CCCGG CC^[GC]GG
NcoI 695 CCATGG C^CATGG
NgoMIV 331 GCCGGC G^CCGGC
NheI 2461 GCTAGC G^CTAGC
NsiI 1903 ATGCAT ATGCA^T
PmeI 3548 GTTTAAAC GTTT^AAAC
SacI 1000 GAGCTC GAGCT^C
SspBI 3443 TGTACA T^GTACA
StuI 2069 AGGCCT AGG^CCT
XcmI 3715 CCAAAAGTAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 1871 GACGCC G[GA]^CG[CT]C
3477 GGCGTC
AflIII 1378 ACATGT A^C[GA][CT]GT
1695 ACATGT
ApaI 699 GGGCCC GGGCC^C
3134 GGGCCC
AvaI 287 CTCGAG C^[CT]CG[GA]G
2578 CTCGAG
AvaII 1539 GGTCC G^G[AT]CC
2006 GGACC
BciVI 3418 GGATAC GTATCC(6/5)
1644 GTATCC
BglII 2250 AGATCT A^GATCT
2942 AGATCT
BlpI 660 GCTTAGC GC^T[ACGT]AGC
1002 GCTCAGC
Bpu1102I 660 GCTTAGC GC^T[ACGT]AGC
1002 GCTCAGC
BsaAI 2136 TACGTG [CT]AC^GT[GA]
2716 TACGTG
BsaHI 1871 GACGCC G[GA]^CG[CT]C
3477 GGCGTC
BseRI 1415 GAGGAG GAGGAG(10/8)
3103 GAGGAG
BsmFI 372 GGGAC GGGAC(10/14)
2005 GGGAC
BsoBI 287 CTCGAG C^[CT]CG[GA]G
2578 CTCGAG
BspLU11I 1378 ACATGT A^CATGT
1695 ACATGT
BstXI 1724 CCAAGTAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2224 CCATGTATATGG
CelII 660 GCTTAGC GC^T[ACGT]AGC
1002 GCTCAGC
EcoT14I 695 CCATGG C^C[AT][AT]GG
1817 CCAAGG
HapII 157 CCGG C^CGG
332 CCGG
Hin1I 1871 GACGCC G[GA]^CG[CT]C
3477 GGCGTC
HindIII 750 AAGCTT A^AGCTT
1716 AAGCTT
HpaII 157 CCGG C^CGG
332 CCGG
MaeII 2137 ACGT A^CGT
2717 ACGT
MspI 157 CCGG C^CGG
332 CCGG
PaeR7I 287 CTCGAG C^TCGAG
2578 CTCGAG
PpuMI 1538 GGGTCCT [GA]G^G[AT]CC[CT]
2005 GGGACCC
PstI 262 CTGCAG CTGCA^G
3601 CTGCAG
StyI 695 CCATGG C^C[AT][AT]GG
1817 CCAAGG
XhoI 287 CTCGAG C^TCGAG
2578 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AseI AsnI Asp700I AspHI BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HgaI HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PshBI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI