##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI Alw44I
ApaLI AscI Asp718I AspHI AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BglI BlnI
BlpI Bpu1102I BsaI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BspDI
BspEI BspLU11I BssHII Bst1107I BstAPI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraII DrdI EaeI
EagI EclXI Eco52I EcoO109I EcoO65I
EcoRV FseI FspI HgaI KpnI
KspI MluI MluNI MroI MscI
NdeI NheI NotI NruI PacI
PflFI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SmaI SnaBI SphI Sse8387I
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AcyI 191 GGCGCC G[GA]^CG[CT]C
AflII 1352 CTTAAG C^TTAAG
AgeI 1270 ACCGGT A^CCGGT
AhdI 1675 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2288 CCTGAGG CC^T[ACGT]AGG
Aor51HI 120 AGCGCT AGC^GCT
ApaI 4 GGGCCC GGGCC^C
AseI 2820 ATTAAT AT^TAAT
AsnI 2820 ATTAAT AT^TAAT
AspEI 1675 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 249 CTCGAG C^[CT]CG[GA]G
BanI 191 GGCGCC G^G[CT][GA]CC
BanII 4 GGGCCC G[GA]GC[CT]^C
BbeI 191 GGCGCC GGCGC^C
BfrI 1352 CTTAAG C^TTAAG
BglII 263 AGATCT A^GATCT
BmyI 4 GGGCCC G[AGT]GC[ACT]^C
BpmI 1395 CTGGAG CTGGAG(16/14)
BsaBI 2454 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 191 GGCGCC G[GA]^CG[CT]C
BsaWI 1270 ACCGGT [AT]^CCGG[AT]
BsoBI 249 CTCGAG C^[CT]CG[GA]G
Bsp1286I 4 GGGCCC G[AGT]GC[ACT]^C
BspMI 836 ACCTGC ACCTGC(4/8)
BsrBI 1000 CCGCTC CCGCTC(-3/-3)
BssSI 46 CACGAG CACGAG(-5/-1)
Bsu36I 2288 CCTGAGG CC^T[ACGT]AGG
DraIII 572 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 183 CCATGG C^C[GA][CT]GG
Eam1105I 1675 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 95 CTCTTC CTCTTC(1/4)
Eco47III 120 AGCGCT AGC^GCT
Eco81I 2288 CCTGAGG CC^T[ACGT]AGG
Hin1I 191 GGCGCC G[GA]^CG[CT]C
HindIII 679 AAGCTT A^AGCTT
HpaI 2657 GTTAAC GTT^AAC
KasI 191 GGCGCC G^GCGCC
Ksp632I 95 CTCTTC CTCTTC(1/4)
MamI 2454 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3123 CAATTG C^AATTG
MunI 3123 CAATTG C^AATTG
NaeI 194 GCCGGC GCC^GGC
NarI 191 GGCGCC GG^CGCC
NcoI 183 CCATGG C^CATGG
NgoMIV 194 GCCGGC G^CCGGC
NspI 2519 GCATGT [GA]CATG^[CT]
PaeR7I 249 CTCGAG C^TCGAG
PflMI 1388 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1270 ACCGGT A^CCGGT
PshBI 2820 ATTAAT AT^TAAT
PstI 2621 CTGCAG CTGCA^G
PvuII 2564 CAGCTG CAG^CTG
SalI 915 GTCGAC G^TCGAC
SfoI 191 GGCGCC GGC^GCC
SgrAI 193 CGCCGGCG C[GA]^CCGG[CT]G
StuI 351 AGGCCT AGG^CCT
SwaI 3296 ATTTAAAT ATTT^AAAT
Van91I 1388 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3344 TCTAGA T^CTAGA
XcmI 802 CCAACCTCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 249 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 915 GTCGAC GT^[AC][GT]AC
2523 GTAGAC
AflIII 2250 ACGTGT A^C[GA][CT]GT
2443 ACGTGT
Asp700I 857 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3169 GAATATATTC
BclI 1036 TGATCA T^GATCA
3036 TGATCA
BsaAI 779 CACGTA [CT]AC^GT[GA]
3110 TACGTG
BsmFI 171 GGGAC GGGAC(10/14)
1728 GTCCC
BsmI 2326 GAATGC GAATGC(1/-1)
3564 GCATTC
Bsp1407I 3738 TGTACA T^GTACA
3745 TGTACA
BspHI 1057 TCATGA T^CATGA
3033 TCATGA
BsrFI 194 GCCGGC [GA]^CCGG[CT]
1270 ACCGGT
BsrGI 3738 TGTACA T^GTACA
3745 TGTACA
BstBI 1569 TTCGAA TT^CGAA
2974 TTCGAA
BstYI 263 AGATCT [GA]^GATC[CT]
3781 GGATCT
Cfr10I 194 GCCGGC [GA]^CCGG[CT]
1270 ACCGGT
EcoNI 2597 CCTATACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2617 CCTACTGCAGG
EcoRI 1168 GAATTC G^AATTC
1772 GAATTC
EcoT14I 183 CCATGG C^C[AT][AT]GG
468 CCTTGG
EcoT22I 3335 ATGCAT ATGCA^T
3562 ATGCAT
FbaI 1036 TGATCA T^GATCA
3036 TGATCA
HaeII 120 AGCGCT [GA]GCGC^[CT]
191 GGCGCC
HincII 915 GTCGAC GT[CT]^[GA]AC
2657 GTTAAC
HindII 915 GTCGAC GT[CT]^[GA]AC
2657 GTTAAC
MflI 263 AGATCT [GA]^GATC[CT]
3781 GGATCT
NsiI 3335 ATGCAT ATGCA^T
3562 ATGCAT
NspV 1569 TTCGAA TT^CGAA
2974 TTCGAA
RcaI 1057 TCATGA T^CATGA
3033 TCATGA
SfuI 1569 TTCGAA TT^CGAA
2974 TTCGAA
SpeI 2138 ACTAGT A^CTAGT
3191 ACTAGT
SspBI 3738 TGTACA T^GTACA
3745 TGTACA
StyI 183 CCATGG C^C[AT][AT]GG
468 CCTTGG
XhoII 263 AGATCT [GA]^GATC[CT]
3781 GGATCT
XmnI 857 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3169 GAATATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BbsI
BbvI BcnI BfaI BpuAI BsaJI
BsgI BsiYI BslI BsmAI BsrDI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I