##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AspEI AviII
AvrII BbrPI BbvCI BcgI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraIII
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HpaI
MamI MroI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SalI
SbfI SfiI SfuI SmaI SnaBI
SphI Sse8387I SwaI TaqI TthHB8I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 727 GGTACC G^GTACC
AclI 292 AACGTT AA^CGTT
AlwNI 2302 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 842 CCTTAGG CC^T[ACGT]AGG
ApaI 89 GGGCCC GGGCC^C
AseI 2480 ATTAAT AT^TAAT
AsnI 2480 ATTAAT AT^TAAT
Asp718I 727 GGTACC G^GTACC
AspI 2664 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 232 CCCGAG C^[CT]CG[GA]G
BalI 663 TGGCCA TGG^CCA
BsaI 391 GAGACC GGTCTC(1/5)
BseRI 3555 GAGGAG GAGGAG(10/8)
BsiEI 7 CGGCCG CG[GA][CT]^CG
BsoBI 232 CCCGAG C^[CT]CG[GA]G
BspLU11I 1137 ACATGT A^CATGT
BsrDI 642 CATTGC GCAATG(2/0)
BsrFI 50 GCCGGT [GA]^CCGG[CT]
BstAPI 1576 GCAGCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 842 CCTTAGG CC^T[ACGT]AGG
Cfr10I 50 GCCGGT [GA]^CCGG[CT]
EagI 7 CGGCCG C^GGCCG
EclXI 7 CGGCCG C^GGCCG
Eco52I 7 CGGCCG C^GGCCG
Eco81I 842 CCTTAGG CC^T[ACGT]AGG
HindIII 1246 AAGCTT A^AGCTT
KpnI 727 GGTACC GGTAC^C
MaeII 293 ACGT A^CGT
MfeI 1724 CAATTG C^AATTG
MluI 193 ACGCGT A^CGCGT
MluNI 663 TGGCCA TGG^CCA
MscI 663 TGGCCA TGG^CCA
MslI 741 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1724 CAATTG C^AATTG
NcoI 3324 CCATGG C^CATGG
NspI 1137 ACATGT [GA]CATG^[CT]
PflFI 2664 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1769 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 75 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 2480 ATTAAT AT^TAAT
Psp1406I 292 AACGTT AA^CGTT
PvuII 311 CAGCTG CAG^CTG
SacI 3197 GAGCTC GAGCT^C
SapI 3199 GCTCTTC GCTCTTC(1/4)
SexAI 3640 ACCAGGT A^CC[AT]GGT
SgrAI 49 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 1570 ACTAGT A^CTAGT
StuI 1851 AGGCCT AGG^CCT
Tth111I 2664 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1769 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2547 CCAGTTCCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1000 GTCTAC GT^[AC][GT]AC
2865 GTAGAC
AcyI 154 GGCGCC G[GA]^CG[CT]C
204 GGCGCC
AflII 1249 CTTAAG C^TTAAG
3049 CTTAAG
AflIII 193 ACGCGT A^C[GA][CT]GT
1137 ACATGT
Asp700I 848 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2377 GAAAACCTTC
BbeI 154 GGCGCC GGCGC^C
204 GGCGCC
BciVI 1133 GGATAC GTATCC(6/5)
1794 GGATAC
BclI 399 TGATCA T^GATCA
1718 TGATCA
BfrI 1249 CTTAAG C^TTAAG
3049 CTTAAG
BpmI 257 CTCCAG CTGGAG(16/14)
950 CTGGAG
BsaHI 154 GGCGCC G[GA]^CG[CT]C
204 GGCGCC
BsmFI 130 GGGAC GGGAC(10/14)
77 GTCCC
Bsp1407I 686 TGTACA T^GTACA
3409 TGTACA
BspHI 3525 TCATGA T^CATGA
3546 TCATGA
BsrGI 686 TGTACA T^GTACA
3409 TGTACA
DraI 2047 TTTAAA TTT^AAA
3394 TTTAAA
DraII 75 AGGTCCC [GA]G^G[ACGT]CC[CT]
89 GGGCCCC
EaeI 7 CGGCCG [CT]^GGCC[GA]
663 TGGCCA
EcoO109I 75 AGGTCCC [GA]G^G[ACGT]CC[CT]
89 GGGCCCC
FbaI 399 TGATCA T^GATCA
1718 TGATCA
HaeII 154 GGCGCC [GA]GCGC^[CT]
204 GGCGCC
Hin1I 154 GGCGCC G[GA]^CG[CT]C
204 GGCGCC
HincII 790 GTTGAC GT[CT]^[GA]AC
2074 GTCAAC
HindII 790 GTTGAC GT[CT]^[GA]AC
2074 GTCAAC
KasI 154 GGCGCC G^GCGCC
204 GGCGCC
NarI 154 GGCGCC GG^CGCC
204 GGCGCC
RcaI 3525 TCATGA T^CATGA
3546 TCATGA
SfoI 154 GGCGCC GGC^GCC
204 GGCGCC
SmlI 1249 CTTAAG C^T[CT][GA]AG
3049 CTTAAG
SspBI 686 TGTACA T^GTACA
3409 TGTACA
SspI 2357 AATATT AAT^ATT
3418 AATATT
XmnI 848 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2377 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BamHI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EarI
EcoNI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI SacII Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII