##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccIII AcsI AflII AgeI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AviII BbeI
BbrPI BfrI BsaAI BseAI BsiEI
BsiWI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
FseI FspI HpaI KasI KpnI
KspI MroI NarI NdeI NotI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PstI
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Van91I
XcmI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 3295 GACGTC GACGT^C
AccI 3313 GTAGAC GT^[AC][GT]AC
AclI 3253 AACGTT AA^CGTT
AhdI 3379 GACGGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3379 GACGGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 801 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1623 CCTAGG C^CTAGG
BamHI 1575 GGATCC G^GATCC
BbvCI 1607 GCTGAGG CCTCAGC(-5/-2)
BcgI 3608 GCATGAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2508 GGATAC GTATCC(6/5)
BclI 712 TGATCA T^GATCA
BglII 3664 AGATCT A^GATCT
BlnI 1623 CCTAGG C^CTAGG
BsaWI 157 TCCGGT [AT]^CCGG[AT]
BspLU11I 916 ACATGT A^CATGT
BsrBI 987 GAGCGG CCGCTC(-3/-3)
BsrFI 23 GCCGGC [GA]^CCGG[CT]
BstAPI 3036 GCACTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 23 GCCGGC [GA]^CCGG[CT]
DrdI 304 GACCTAACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3379 GACGGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 629 GATATC GAT^ATC
EcoT22I 1147 ATGCAT ATGCA^T
FbaI 712 TGATCA T^GATCA
HincII 594 GTCAAC GT[CT]^[GA]AC
HindII 594 GTCAAC GT[CT]^[GA]AC
MfeI 2948 CAATTG C^AATTG
MunI 2948 CAATTG C^AATTG
NaeI 23 GCCGGC GCC^GGC
NgoMIV 23 GCCGGC G^CCGGC
NheI 3436 GCTAGC G^CTAGC
NruI 2895 TCGCGA TCG^CGA
NsiI 1147 ATGCAT ATGCA^T
PaeR7I 3616 CTCGAG C^TCGAG
PflFI 801 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3253 AACGTT AA^CGTT
PvuII 2194 CAGCTG CAG^CTG
SapI 1023 GAAGAGC GCTCTTC(1/4)
SexAI 2512 ACCTGGT A^CC[AT]GGT
SphI 1637 GCATGC GCATG^C
Tth111I 801 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3616 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 564 GACGCC G[GA]^CG[CT]C
3295 GACGTC
AocI 839 CCTGAGG CC^T[ACGT]AGG
1909 CCTCAGG
Asp700I 422 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2899 GAACGATTTC
BlpI 1138 GCTGAGC GC^T[ACGT]AGC
3164 GCTTAGC
Bpu1102I 1138 GCTGAGC GC^T[ACGT]AGC
3164 GCTTAGC
BsaBI 333 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
629 GATATCTATC
BsaHI 564 GACGCC G[GA]^CG[CT]C
3295 GACGTC
BsmBI 3247 CGTCTC CGTCTC(1/5)
1894 GAGACG
Bsp1407I 901 TGTACA T^GTACA
1416 TGTACA
BspHI 715 TCATGA T^CATGA
3722 TCATGA
BspMI 2422 ACCTGC ACCTGC(4/8)
1756 GCAGGT
BsrGI 901 TGTACA T^GTACA
1416 TGTACA
Bsu36I 839 CCTGAGG CC^T[ACGT]AGG
1909 CCTCAGG
CelII 1138 GCTGAGC GC^T[ACGT]AGC
3164 GCTTAGC
Eco81I 839 CCTGAGG CC^T[ACGT]AGG
1909 CCTCAGG
HgaI 564 GACGC GACGC(5/10)
3246 GCGTC
Hin1I 564 GACGCC G[GA]^CG[CT]C
3295 GACGTC
HindIII 3202 AAGCTT A^AGCTT
3933 AAGCTT
MamI 333 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
629 GATATCTATC
MluI 113 ACGCGT A^CGCGT
3244 ACGCGT
MspA1I 2194 CAGCTG C[AC]G^C[GT]G
2523 CAGCGG
NcoI 1463 CCATGG C^CATGG
2059 CCATGG
NspI 916 ACATGT [GA]CATG^[CT]
1637 GCATGC
RcaI 715 TCATGA T^CATGA
3722 TCATGA
SacI 370 GAGCTC GAGCT^C
1026 GAGCTC
SfcI 1426 CTATAG C^T[GA][CT]AG
2012 CTACAG
SspBI 901 TGTACA T^GTACA
1416 TGTACA
XbaI 1347 TCTAGA T^CTAGA
2249 TCTAGA
XmnI 422 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2899 GAACGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI