##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
Asp718I AspEI AviII AvrII BamHI
BanII BbrPI BbvCI BcgI BglI
BlnI BsaAI BsaI BseRI BsiWI
BsmBI BspDI BsrDI BsrFI BssHII
BstEII BstPI Bsu36I Cfr10I ClaI
CpoI DrdI DsaI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV FseI
FspI HpaI KpnI KspI MaeII
MluI NaeI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SbfI SfiI
SgrAI SnaBI Sse8387I StuI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 128 CGCG CG^CG
AccIII 276 TCCGGA T^CCGGA
AcyI 101 GGCGCC G[GA]^CG[CT]C
AflIII 4071 ACATGT A^C[GA][CT]GT
Alw44I 3296 GTGCAC G^TGCAC
ApaLI 3296 GTGCAC G^TGCAC
AspI 254 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 101 GGCGCC GGCGC^C
BglII 2259 AGATCT A^GATCT
BlpI 1529 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1529 GCTGAGC GC^T[ACGT]AGC
BsaBI 3241 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 101 GGCGCC G[GA]^CG[CT]C
BseAI 276 TCCGGA T^CCGGA
BsiEI 886 CGGCCG CG[GA][CT]^CG
BsmI 2523 GAATGC GAATGC(1/-1)
BspEI 276 TCCGGA T^CCGGA
BspLU11I 4071 ACATGT A^CATGT
BsrBI 883 GAGCGG CCGCTC(-3/-3)
BssSI 82 CTCGTG CACGAG(-5/-1)
Bst1107I 3286 GTATAC GTA^TAC
BstAPI 779 GCAGGTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 244 TTCGAA TT^CGAA
BstUI 128 CGCG CG^CG
BstZ17I 3286 GTATAC GTA^TAC
CelII 1529 GCTGAGC GC^T[ACGT]AGC
DraIII 1793 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 886 CGGCCG C^GGCCG
EclXI 886 CGGCCG C^GGCCG
Eco52I 886 CGGCCG C^GGCCG
FauI 220 GCGGG CCCGC(4/6)
HgaI 40 GACGC GACGC(5/10)
Hin1I 101 GGCGCC G[GA]^CG[CT]C
KasI 101 GGCGCC G^GCGCC
MamI 3241 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 276 TCCGGA T^CCGGA
MvnI 128 CGCG CG^CG
NarI 101 GGCGCC GG^CGCC
NdeI 492 CATATG CA^TATG
NspV 244 TTCGAA TT^CGAA
PflFI 254 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1792 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2999 GACATAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1862 GAAGAGC GCTCTTC(1/4)
SexAI 1125 ACCTGGT A^CC[AT]GGT
SfoI 101 GGCGCC GGC^GCC
SfuI 244 TTCGAA TT^CGAA
SpeI 4195 ACTAGT A^CTAGT
Tth111I 254 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1792 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AflII 2531 CTTAAG C^TTAAG
3059 CTTAAG
AseI 1049 ATTAAT AT^TAAT
2686 ATTAAT
AsnI 1049 ATTAAT AT^TAAT
2686 ATTAAT
Asp700I 3461 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3787 GAAGAGTTTC
AspHI 77 GTGCTC G[AT]GC[AT]^C
3296 GTGCAC
AvaI 23 CCCGGG C^[CT]CG[GA]G
139 CCCGGG
BalI 772 TGGCCA TGG^CCA
1197 TGGCCA
BanI 101 GGCGCC G^G[CT][GA]CC
2161 GGCACC
BciVI 279 GGATAC GTATCC(6/5)
3479 GGATAC
BclI 1825 TGATCA T^GATCA
3861 TGATCA
BfrI 2531 CTTAAG C^TTAAG
3059 CTTAAG
BsaWI 276 TCCGGA [AT]^CCGG[AT]
714 ACCGGA
BsiHKAI 77 GTGCTC G[AT]GC[AT]^C
3296 GTGCAC
BsoBI 23 CCCGGG C^[CT]CG[GA]G
139 CCCGGG
BspHI 1828 TCATGA T^CATGA
3858 TCATGA
DraII 142 GGGACCC [GA]G^G[ACGT]CC[CT]
201 AGGACCT
EcoNI 501 CCTTTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1056 CCTTCAAGAGG
EcoO109I 142 GGGACCC [GA]G^G[ACGT]CC[CT]
201 AGGACCT
FbaI 1825 TGATCA T^GATCA
3861 TGATCA
HaeII 101 GGCGCC [GA]GCGC^[CT]
1833 AGCGCC
MfeI 3519 CAATTG C^AATTG
4012 CAATTG
MluNI 772 TGGCCA TGG^CCA
1197 TGGCCA
MscI 772 TGGCCA TGG^CCA
1197 TGGCCA
MspA1I 686 CAGCTG C[AC]G^C[GT]G
3291 CAGCTG
MunI 3519 CAATTG C^AATTG
4012 CAATTG
PpuMI 142 GGGACCC [GA]G^G[AT]CC[CT]
201 AGGACCT
PshBI 1049 ATTAAT AT^TAAT
2686 ATTAAT
PvuII 686 CAGCTG CAG^CTG
3291 CAGCTG
RcaI 1828 TCATGA T^CATGA
3858 TCATGA
SalI 465 GTCGAC G^TCGAC
1018 GTCGAC
ScaI 2324 AGTACT AGT^ACT
3994 AGTACT
SmaI 23 CCCGGG CCC^GGG
139 CCCGGG
XbaI 2392 TCTAGA T^CTAGA
4055 TCTAGA
XcmI 512 CCAAAGGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1155 CCACTGCTTGTGTGG
XmaCI 23 CCCGGG C^CCGGG
139 CCCGGG
XmaI 23 CCCGGG C^CCGGG
139 CCCGGG
XmnI 3461 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3787 GAAGAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AvaII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII