##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI Aor51HI ApaI AscI Asp718I
AspI AviII AvrII BalI BanI
BbeI BglI BlnI BsaBI BsaHI
BseAI BsiWI BspEI BspHI BsrBI
BssHII BssSI Bst1107I BstUI BstXI
BstZ17I CfoI CpoI EaeI EagI
EclXI Eco47III Eco52I EcoNI EcoT22I
FseI FspI HaeII HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflFI PmeI Psp1406I RcaI
RsrII SacI SacII SalI SbfI
SfiI SfoI SgrAI SmaI SphI
Sse8387I StuI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccI 1737 GTCTAC GT^[AC][GT]AC
AgeI 820 ACCGGT A^CCGGT
AhdI 2689 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 365 GTGCAC G^TGCAC
AlwNI 1697 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 365 GTGCAC G^TGCAC
AseI 1246 ATTAAT AT^TAAT
AsnI 1246 ATTAAT AT^TAAT
Asp700I 3973 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2689 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 272 CTCGGG C^[CT]CG[GA]G
BamHI 826 GGATCC G^GATCC
BbrPI 3261 CACGTG CAC^GTG
BcgI 1437 GCAAGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 919 GGATAC GTATCC(6/5)
BcnI 654 CCGGG CC^[GC]GG
BlpI 1018 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1018 GCTGAGC GC^T[ACGT]AGC
BsaI 4706 GAGACC GGTCTC(1/5)
BsaWI 820 ACCGGT [AT]^CCGG[AT]
BsgI 3324 CTGCAC GTGCAG(16/14)
BsiEI 3061 CGATCG CG[GA][CT]^CG
BsmBI 1833 CGTCTC CGTCTC(1/5)
BsoBI 272 CTCGGG C^[CT]CG[GA]G
BspDI 3059 ATCGAT AT^CGAT
BsrFI 820 ACCGGT [GA]^CCGG[CT]
BstBI 703 TTCGAA TT^CGAA
BstEII 1045 GGTGACC G^GT[ACGT]ACC
BstPI 1045 GGTGACC G^GT[ACGT]ACC
CelII 1018 GCTGAGC GC^T[ACGT]AGC
Cfr10I 820 ACCGGT [GA]^CCGG[CT]
ClaI 3059 ATCGAT AT^CGAT
DraIII 1483 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 772 GACAACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2689 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1045 GGTGACC G^GT[ACGT]ACC
EcoRI 3512 GAATTC G^AATTC
FauI 4454 CCCGC CCCGC(4/6)
HgaI 2712 GACGC GACGC(5/10)
HincII 938 GTCAAC GT[CT]^[GA]AC
HindII 938 GTCAAC GT[CT]^[GA]AC
NciI 654 CCGGG CC^[GC]GG
NcoI 3395 CCATGG C^CATGG
NdeI 2090 CATATG CA^TATG
NheI 460 GCTAGC G^CTAGC
NspV 703 TTCGAA TT^CGAA
PflMI 3493 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 820 ACCGGT A^CCGGT
PmaCI 3261 CACGTG CAC^GTG
PmlI 3261 CACGTG CAC^GTG
PshAI 2460 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1246 ATTAAT AT^TAAT
PvuI 3061 CGATCG CGAT^CG
ScaI 2356 AGTACT AGT^ACT
SfuI 703 TTCGAA TT^CGAA
SnaBI 3420 TACGTA TAC^GTA
SpeI 2143 ACTAGT A^CTAGT
SwaI 4202 ATTTAAAT ATTT^AAAT
Van91I 3493 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 758 CCACAGTGATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3973 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflII 1918 CTTAAG C^TTAAG
3139 CTTAAG
AocI 187 CCTGAGG CC^T[ACGT]AGG
3214 CCTGAGG
BclI 1074 TGATCA T^GATCA
4271 TGATCA
BfrI 1918 CTTAAG C^TTAAG
3139 CTTAAG
BglII 841 AGATCT A^GATCT
4524 AGATCT
BsaAI 3261 CACGTG [CT]AC^GT[GA]
3420 TACGTA
BsmFI 57 GTCCC GGGAC(10/14)
790 GTCCC
Bsp1407I 738 TGTACA T^GTACA
4409 TGTACA
BspMI 369 ACCTGC ACCTGC(4/8)
2518 GCAGGT
BsrGI 738 TGTACA T^GTACA
4409 TGTACA
BstAPI 255 GCAAATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3403 GCATTTTTTGC
Bsu36I 187 CCTGAGG CC^T[ACGT]AGG
3214 CCTGAGG
DraII 804 AGGACCT [GA]G^G[ACGT]CC[CT]
1352 AGGACCT
DsaI 634 CCACGG C^C[GA][CT]GG
3395 CCATGG
Eco81I 187 CCTGAGG CC^T[ACGT]AGG
3214 CCTGAGG
EcoO109I 804 AGGACCT [GA]G^G[ACGT]CC[CT]
1352 AGGACCT
EcoRV 852 GATATC GAT^ATC
4917 GATATC
FbaI 1074 TGATCA T^GATCA
4271 TGATCA
HindIII 3334 AAGCTT A^AGCTT
3604 AAGCTT
MspA1I 998 CAGCTG C[AC]G^C[GT]G
1697 CAGCTG
NlaIV 826 GGATCC GG[ACGT]^[ACGT]CC
3000 GGAACC
PpuMI 804 AGGACCT [GA]G^G[AT]CC[CT]
1352 AGGACCT
PstI 1111 CTGCAG CTGCA^G
3297 CTGCAG
PvuII 998 CAGCTG CAG^CTG
1697 CAGCTG
SapI 1323 GCTCTTC GCTCTTC(1/4)
2776 GCTCTTC
SspBI 738 TGTACA T^GTACA
4409 TGTACA
XbaI 1572 TCTAGA T^CTAGA
4189 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BanII
BbsI BbvCI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmI Bsp1286I
BspLU11I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NspI PleI
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII