##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspEI
AviII AvrII BalI BamHI BbrPI
BcgI BfrI BlnI BsaAI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspEI
BspLU11I BspMI BsrBI BsrGI BssSI
Bst1107I BstEII BstPI BstZ17I DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FseI
FspI HgaI HpaI KspI MfeI
MluI MluNI MroI MscI MspA1I
MunI NcoI NdeI NotI NruI
PacI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AocI 601 CCTCAGG CC^T[ACGT]AGG
ApaI 205 GGGCCC GGGCC^C
AspHI 2591 GAGCAC G[AT]GC[AT]^C
AspI 691 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 433 GCTGAGG CCTCAGC(-5/-2)
BglI 584 GCCGCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 180 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 180 GCTGAGC GC^T[ACGT]AGC
BsaBI 3352 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2460 GGTCTC GGTCTC(1/5)
BsgI 856 GTGCAG GTGCAG(16/14)
BsiEI 1962 CGATCG CG[GA][CT]^CG
BsiHKAI 2591 GAGCAC G[AT]GC[AT]^C
BsmI 3822 GCATTC GAATGC(1/-1)
BspDI 1920 ATCGAT AT^CGAT
BsrFI 223 GCCGGC [GA]^CCGG[CT]
BssHII 158 GCGCGC G^CGCGC
BstAPI 119 GCAGGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3410 TTCGAA TT^CGAA
Bsu36I 601 CCTCAGG CC^T[ACGT]AGG
CelII 180 GCTGAGC GC^T[ACGT]AGC
Cfr10I 223 GCCGGC [GA]^CCGG[CT]
ClaI 1920 ATCGAT AT^CGAT
CpoI 1318 CGGTCCG CG^G[AT]CCG
DraIII 830 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 601 CCTCAGG CC^T[ACGT]AGG
EcoT22I 3820 ATGCAT ATGCA^T
HincII 802 GTTGAC GT[CT]^[GA]AC
HindII 802 GTTGAC GT[CT]^[GA]AC
HindIII 1705 AAGCTT A^AGCTT
MamI 3352 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 223 GCCGGC GCC^GGC
NgoMIV 223 GCCGGC G^CCGGC
NheI 654 GCTAGC G^CTAGC
NsiI 3820 ATGCAT ATGCA^T
NspI 1394 ACATGC [GA]CATG^[CT]
NspV 3410 TTCGAA TT^CGAA
PaeR7I 238 CTCGAG C^TCGAG
PflFI 691 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 898 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 550 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 809 CTGCAG CTGCA^G
PvuI 1962 CGATCG CGAT^CG
RsrII 1318 CGGTCCG CG^G[AT]CCG
SfuI 3410 TTCGAA TT^CGAA
Tth111I 691 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 898 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 238 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 247 GGCGCC G[GA]^CG[CT]C
302 GGCGCC
AlwNI 1285 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2189 CAGGAACTG
Asp700I 961 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1014 GAAGACTTTC
BbeI 247 GGCGCC GGCGC^C
302 GGCGCC
BciVI 2544 GGATAC GTATCC(6/5)
3377 GTATCC
BglII 1115 AGATCT A^GATCT
2579 AGATCT
BsaHI 247 GGCGCC G[GA]^CG[CT]C
302 GGCGCC
BspHI 2175 TCATGA T^CATGA
3506 TCATGA
BsrDI 3106 GCAATG GCAATG(2/0)
3647 GCAATG
BstXI 756 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3065 CCAATATTGTGG
DraII 204 GGGGCCC [GA]G^G[ACGT]CC[CT]
205 GGGCCCC
EarI 2896 CTCTTC CTCTTC(1/4)
3203 CTCTTC
EcoNI 49 CCTGGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
164 CCTGCGGGAGG
EcoO109I 204 GGGGCCC [GA]G^G[ACGT]CC[CT]
205 GGGCCCC
Hin1I 247 GGCGCC G[GA]^CG[CT]C
302 GGCGCC
KasI 247 GGCGCC G^GCGCC
302 GGCGCC
Ksp632I 2896 CTCTTC CTCTTC(1/4)
3203 CTCTTC
MaeII 1590 ACGT A^CGT
3963 ACGT
NarI 247 GGCGCC GG^CGCC
302 GGCGCC
RcaI 2175 TCATGA T^CATGA
3506 TCATGA
SfcI 809 CTGCAG C^T[GA][CT]AG
1524 CTACAG
SfoI 247 GGCGCC GGC^GCC
302 GGCGCC
SspI 682 AATATT AAT^ATT
3067 AATATT
XmnI 961 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1014 GAAGACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AluI AlwI ApoI Asp718I AvaI
AvaII BanI BanII BbsI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EcoRI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI KpnI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI