##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Aor51HI ApaI AscI Asp718I AspEI
AvaI AviII AvrII BamHI BcgI
BlnI BlpI Bpu1102I BsaBI BsiEI
BsiWI BsmBI BsoBI BspDI BspMI
BsrBI BsrFI BssHII Bst1107I BstBI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV FseI
FspI HpaI KpnI KspI MamI
MluI NaeI NcoI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 1370 GTCTAC GT^[AC][GT]AC
AccII 1484 CGCG CG^CG
AccIII 58 TCCGGA T^CCGGA
Alw44I 3071 GTGCAC G^TGCAC
AlwNI 29 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 3071 GTGCAC G^TGCAC
Asp700I 1906 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 109 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 488 GAGCTC G[GA]GC[CT]^C
BbrPI 1502 CACGTG CAC^GTG
BglI 3334 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 675 AGATCT A^GATCT
BsaWI 58 TCCGGA [AT]^CCGG[AT]
BseAI 58 TCCGGA T^CCGGA
BspEI 58 TCCGGA T^CCGGA
BspHI 4003 TCATGA T^CATGA
BspLU11I 1955 ACATGT A^CATGT
BsrDI 3042 CATTGC GCAATG(2/0)
BssSI 2653 CTCGTG CACGAG(-5/-1)
BstAPI 40 GCATCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4099 GGTAACC G^GT[ACGT]ACC
BstPI 4099 GGTAACC G^GT[ACGT]ACC
BstUI 1484 CGCG CG^CG
BstXI 1525 CCACAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 390 CCTGACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4099 GGTAACC G^GT[ACGT]ACC
EcoT22I 4048 ATGCAT ATGCA^T
HincII 3318 GTTGAC GT[CT]^[GA]AC
HindII 3318 GTTGAC GT[CT]^[GA]AC
MroI 58 TCCGGA T^CCGGA
MspA1I 50 CAGCTG C[AC]G^C[GT]G
MvnI 1484 CGCG CG^CG
NdeI 4945 CATATG CA^TATG
NheI 4730 GCTAGC G^CTAGC
NsiI 4048 ATGCAT ATGCA^T
PflFI 109 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2791 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1502 CACGTG CAC^GTG
PmlI 1502 CACGTG CAC^GTG
PpuMI 2417 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 4501 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 50 CAGCTG CAG^CTG
RcaI 4003 TCATGA T^CATGA
SacI 488 GAGCTC GAGCT^C
SexAI 3123 ACCTGGT A^CC[AT]GGT
SpeI 4402 ACTAGT A^CTAGT
Tth111I 109 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2791 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1313 CCAATGTGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1906 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 197 GGCGCC G[GA]^CG[CT]C
2306 GGCGCC
AflII 3780 CTTAAG C^TTAAG
4470 CTTAAG
AocI 148 CCTGAGG CC^T[ACGT]AGG
4066 CCTGAGG
AseI 1543 ATTAAT AT^TAAT
2257 ATTAAT
AsnI 1543 ATTAAT AT^TAAT
2257 ATTAAT
BalI 689 TGGCCA TGG^CCA
3341 TGGCCA
BbeI 197 GGCGCC GGCGC^C
2306 GGCGCC
BbsI 860 GAAGAC GAAGAC(2/6)
2482 GAAGAC
BbvCI 2402 CCTCAGC CCTCAGC(-5/-2)
34 GCTGAGG
BciVI 1234 GGATAC GTATCC(6/5)
1723 GGATAC
BclI 570 TGATCA T^GATCA
1424 TGATCA
BfrI 3780 CTTAAG C^TTAAG
4470 CTTAAG
BpuAI 860 GAAGAC GAAGAC(2/6)
2482 GAAGAC
BsaAI 1502 CACGTG [CT]AC^GT[GA]
2463 TACGTG
BsaHI 197 GGCGCC G[GA]^CG[CT]C
2306 GGCGCC
BsaI 364 GAGACC GGTCTC(1/5)
3127 GGTCTC
BseRI 2546 CTCCTC GAGGAG(10/8)
1336 GAGGAG
BsgI 23 GTGCAG GTGCAG(16/14)
47 GTGCAG
BsiYI 390 CCTGACAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2791 CCAGCCTTTGG
BslI 390 CCTGACAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2791 CCAGCCTTTGG
Bsp1407I 1360 TGTACA T^GTACA
4480 TGTACA
BsrGI 1360 TGTACA T^GTACA
4480 TGTACA
Bsu36I 148 CCTGAGG CC^T[ACGT]AGG
4066 CCTGAGG
EaeI 689 TGGCCA [CT]^GGCC[GA]
3341 TGGCCA
EarI 2096 CTCTTC CTCTTC(1/4)
4167 GAAGAG
Eco81I 148 CCTGAGG CC^T[ACGT]AGG
4066 CCTGAGG
FauI 226 GCGGG CCCGC(4/6)
230 GCGGG
FbaI 570 TGATCA T^GATCA
1424 TGATCA
HaeII 197 GGCGCC [GA]GCGC^[CT]
2306 GGCGCC
Hin1I 197 GGCGCC G[GA]^CG[CT]C
2306 GGCGCC
KasI 197 GGCGCC G^GCGCC
2306 GGCGCC
Ksp632I 2096 CTCTTC CTCTTC(1/4)
4167 GAAGAG
MluNI 689 TGGCCA TGG^CCA
3341 TGGCCA
MscI 689 TGGCCA TGG^CCA
3341 TGGCCA
NarI 197 GGCGCC GG^CGCC
2306 GGCGCC
NspI 1366 GCATGT [GA]CATG^[CT]
1955 ACATGT
PshBI 1543 ATTAAT AT^TAAT
2257 ATTAAT
PstI 4 CTGCAG CTGCA^G
773 CTGCAG
ScaI 3436 AGTACT AGT^ACT
4328 AGTACT
SfoI 197 GGCGCC GGC^GCC
2306 GGCGCC
SspBI 1360 TGTACA T^GTACA
4480 TGTACA
SspI 4008 AATATT AAT^ATT
4605 AATATT
StuI 152 AGGCCT AGG^CCT
4587 AGGCCT
SwaI 3787 ATTTAAAT ATTT^AAAT
4137 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BanI
BbvI BcnI BfaI BmyI BpmI
BsaJI BsiHKAI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspI
MunI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII