##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AcyI AflII Alw44I Aor51HI
ApaLI AscI AspI AviII BamHI
BbeI BcgI BciVI BfrI BsaHI
BsiWI Bsp1407I BspDI BspHI BsrGI
Bst1107I BstAPI BstZ17I ClaI CpoI
DraIII Eco47III EcoNI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI MfeI MluI MunI NarI
NcoI NruI PacI PflFI PflMI
PmeI PshAI PvuI RcaI RsrII
SacI SalI SbfI ScaI SfiI
SfoI SgrAI Sse8387I SspBI Tth111I
Van91I XbaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 776 GGTACC G^GTACC
AccIII 836 TCCGGA T^CCGGA
AclI 2448 AACGTT AA^CGTT
AgeI 56 ACCGGT A^CCGGT
AhdI 3493 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 225 GGGCCC GGGCC^C
AseI 3162 ATTAAT AT^TAAT
AsnI 3162 ATTAAT AT^TAAT
Asp718I 776 GGTACC G^GTACC
AspEI 3493 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 487 TGGCCA TGG^CCA
BclI 3179 TGATCA T^GATCA
BglII 1502 AGATCT A^GATCT
BlpI 161 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 161 GCTGAGC GC^T[ACGT]AGC
BsaBI 1116 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 836 TCCGGA T^CCGGA
BsgI 825 GTGCAG GTGCAG(16/14)
BsmBI 2751 GAGACG CGTCTC(1/5)
BspEI 836 TCCGGA T^CCGGA
BspMI 4070 ACCTGC ACCTGC(4/8)
BsrBI 253 CCGCTC CCGCTC(-3/-3)
BssHII 303 GCGCGC G^CGCGC
BssSI 2464 CACGAG CACGAG(-5/-1)
BstBI 2419 TTCGAA TT^CGAA
BstXI 3070 CCAGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 161 GCTGAGC GC^T[ACGT]AGC
Eam1105I 3493 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 3654 GATATC GAT^ATC
FbaI 3179 TGATCA T^GATCA
HaeII 158 GGCGCT [GA]GCGC^[CT]
KpnI 776 GGTACC GGTAC^C
MamI 1116 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 487 TGGCCA TGG^CCA
MroI 836 TCCGGA T^CCGGA
MscI 487 TGGCCA TGG^CCA
NdeI 787 CATATG CA^TATG
NheI 4034 GCTAGC G^CTAGC
NspV 2419 TTCGAA TT^CGAA
PaeR7I 465 CTCGAG C^TCGAG
PinAI 56 ACCGGT A^CCGGT
PshBI 3162 ATTAAT AT^TAAT
Psp1406I 2448 AACGTT AA^CGTT
SapI 3352 GAAGAGC GCTCTTC(1/4)
SexAI 2181 ACCTGGT A^CC[AT]GGT
SfuI 2419 TTCGAA TT^CGAA
SmaI 3047 CCCGGG CCC^GGG
SnaBI 1399 TACGTA TAC^GTA
SpeI 2258 ACTAGT A^CTAGT
SphI 3040 GCATGC GCATG^C
StuI 2488 AGGCCT AGG^CCT
SwaI 3757 ATTTAAAT ATTT^AAAT
XhoI 465 CTCGAG C^TCGAG
XmaCI 3047 CCCGGG C^CCGGG
XmaI 3047 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 3311 GTAGAC GT^[AC][GT]AC
3453 GTCTAC
AlwNI 982 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
3685 CAGCTTCTG
AocI 2640 CCTGAGG CC^T[ACGT]AGG
4166 CCTGAGG
AspHI 1766 GAGCAC G[AT]GC[AT]^C
2768 GAGCAC
AvrII 3326 CCTAGG C^CTAGG
4238 CCTAGG
BanII 225 GGGCCC G[GA]GC[CT]^C
2783 GAGCCC
BbrPI 1642 CACGTG CAC^GTG
3305 CACGTG
BbvCI 268 CCTCAGC CCTCAGC(-5/-2)
2410 CCTCAGC
BglI 176 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
501 GCCGAAGTGGC
BlnI 3326 CCTAGG C^CTAGG
4238 CCTAGG
BsiHKAI 1766 GAGCAC G[AT]GC[AT]^C
2768 GAGCAC
BspLU11I 1208 ACATGT A^CATGT
4252 ACATGT
BstEII 115 GGTCACC G^GT[ACGT]ACC
2372 GGTCACC
BstPI 115 GGTCACC G^GT[ACGT]ACC
2372 GGTCACC
Bsu36I 2640 CCTGAGG CC^T[ACGT]AGG
4166 CCTGAGG
DraI 852 TTTAAA TTT^AAA
3758 TTTAAA
DrdI 534 GACGTGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2329 GACCACGAAGTC
Eco81I 2640 CCTGAGG CC^T[ACGT]AGG
4166 CCTGAGG
EcoO65I 115 GGTCACC G^GT[ACGT]ACC
2372 GGTCACC
EcoT22I 645 ATGCAT ATGCA^T
3991 ATGCAT
HindIII 754 AAGCTT A^AGCTT
2308 AAGCTT
NaeI 4107 GCCGGC GCC^GGC
4135 GCCGGC
NgoMIV 4107 GCCGGC G^CCGGC
4135 GCCGGC
NsiI 645 ATGCAT ATGCA^T
3991 ATGCAT
PmaCI 1642 CACGTG CAC^GTG
3305 CACGTG
PmlI 1642 CACGTG CAC^GTG
3305 CACGTG
PpuMI 1231 AGGACCC [GA]G^G[AT]CC[CT]
4067 GGGACCT
PstI 637 CTGCAG CTGCA^G
3691 CTGCAG
SspI 610 AATATT AAT^ATT
4349 AATATT
XcmI 496 CCAAAGCCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3297 CCAGACCCCACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AvaI
AvaII BanI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsiEI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NotI NspI
PleI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI