##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI Alw44I ApaI ApaLI AscI
Asp718I AviII AvrII BbeI BbrPI
BcgI BfrI BglI BlnI BlpI
Bpu1102I BsiEI BsiWI BspDI BsrBI
BsrFI BssHII Bst1107I BstBI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DrdI EagI EclXI Eco52I EcoT22I
FseI FspI HpaI KasI KpnI
KspI MluI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I StuI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 2782 TCCGGA T^CCGGA
AflIII 1262 ACATGT A^C[GA][CT]GT
AocI 2397 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2051 AGCGCT AGC^GCT
AseI 2033 ATTAAT AT^TAAT
AsnI 2033 ATTAAT AT^TAAT
AspI 652 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4419 CCCGAG C^[CT]CG[GA]G
BbvCI 1580 GCTGAGG CCTCAGC(-5/-2)
BciVI 582 GTATCC GTATCC(6/5)
BcnI 2931 CCCGG CC^[GC]GG
BsaAI 2872 TACGTG [CT]AC^GT[GA]
BsaI 3456 GAGACC GGTCTC(1/5)
BseAI 2782 TCCGGA T^CCGGA
BsgI 2941 GTGCAG GTGCAG(16/14)
BsoBI 4419 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2060 TGTACA T^GTACA
BspEI 2782 TCCGGA T^CCGGA
BspHI 3296 TCATGA T^CATGA
BspLU11I 1262 ACATGT A^CATGT
BsrGI 2060 TGTACA T^GTACA
BssSI 2994 CTCGTG CACGAG(-5/-1)
BstEII 3830 GGTCACC G^GT[ACGT]ACC
BstPI 3830 GGTCACC G^GT[ACGT]ACC
BstXI 3038 CCACTTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2397 CCTGAGG CC^T[ACGT]AGG
DraIII 761 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2051 AGCGCT AGC^GCT
Eco81I 2397 CCTGAGG CC^T[ACGT]AGG
EcoNI 4393 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3830 GGTCACC G^GT[ACGT]ACC
EcoRV 2070 GATATC GAT^ATC
FauI 4441 CCCGC CCCGC(4/6)
HincII 2773 GTCGAC GT[CT]^[GA]AC
HindII 2773 GTCGAC GT[CT]^[GA]AC
MfeI 574 CAATTG C^AATTG
MroI 2782 TCCGGA T^CCGGA
MunI 574 CAATTG C^AATTG
NciI 2931 CCCGG CC^[GC]GG
NdeI 2064 CATATG CA^TATG
PflFI 652 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2033 ATTAAT AT^TAAT
RcaI 3296 TCATGA T^CATGA
SacI 1768 GAGCTC GAGCT^C
SalI 2773 GTCGAC G^TCGAC
SapI 159 GAAGAGC GCTCTTC(1/4)
SpeI 669 ACTAGT A^CTAGT
SphI 4835 GCATGC GCATG^C
SspBI 2060 TGTACA T^GTACA
Tth111I 652 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 3892 GACGCC G[GA]^CG[CT]C
4575 GGCGTC
AhdI 2716 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4545 GACTCCCAGTC
AspEI 2716 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4545 GACTCCCAGTC
BanI 1832 GGTGCC G^G[CT][GA]CC
3342 GGTGCC
BanII 1768 GAGCTC G[GA]GC[CT]^C
4502 GGGCTC
BsaBI 633 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
807 GATGCTCATC
BsaHI 3892 GACGCC G[GA]^CG[CT]C
4575 GGCGTC
BsaWI 1732 ACCGGA [AT]^CCGG[AT]
2782 TCCGGA
BstAPI 2292 GCAGGTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3636 GCATTTATTGC
DraI 2487 TTTAAA TTT^AAA
3620 TTTAAA
DsaI 1048 CCATGG C^C[GA][CT]GG
4385 CCATGG
Eam1105I 2716 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4545 GACTCCCAGTC
HaeII 690 GGCGCT [GA]GCGC^[CT]
2051 AGCGCT
Hin1I 3892 GACGCC G[GA]^CG[CT]C
4575 GGCGTC
HindIII 187 AAGCTT A^AGCTT
643 AAGCTT
MaeII 2873 ACGT A^CGT
3506 ACGT
MamI 633 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
807 GATGCTCATC
NcoI 1048 CCATGG C^CATGG
4385 CCATGG
PpuMI 2665 AGGTCCT [GA]G^G[AT]CC[CT]
2722 AGGTCCT
SexAI 2350 ACCAGGT A^CC[AT]GGT
3082 ACCAGGT
SspI 626 AATATT AAT^ATT
830 AATATT
TaqI 1766 TCGA T^CGA
2774 TCGA
TthHB8I 1766 TCGA T^CGA
2774 TCGA
XbaI 182 TCTAGA T^CTAGA
942 TCTAGA
XcmI 763 CCATGTGGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1146 CCACTGCTGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BamHI BbsI BbvI
BclI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII XmnI