##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AccIII AgeI AhdI Alw44I ApaI
ApaLI AscI AspEI AviII AvrII
BbeI BbrPI BciVI BcnI BglII
BlnI BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI Bsp1407I BspEI BspLU11I
BspMI BsrBI BsrGI BssHII CelII
CpoI DraIII EagI Eam1105I EclXI
Eco52I EcoNI EcoRI EcoRV FauI
FseI FspI KasI KspI MfeI
MluI MroI MunI NaeI NarI
NciI NgoMIV NheI NotI NruI
PacI PinAI PmaCI PmeI PmlI
PpuMI PvuI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 74 enzymes) #
###################################
AatII 538 GACGTC GACGT^C
Acc65I 844 GGTACC G^GTACC
AclI 3869 AACGTT AA^CGTT
AflII 2150 CTTAAG C^TTAAG
AflIII 2854 ACGTGT A^C[GA][CT]GT
Aor51HI 1011 AGCGCT AGC^GCT
Asp700I 1239 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 844 GGTACC G^GTACC
AspHI 765 GAGCTC G[AT]GC[AT]^C
AspI 4078 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 138 CTCGAG C^[CT]CG[GA]G
BalI 153 TGGCCA TGG^CCA
BamHI 1124 GGATCC G^GATCC
BanI 844 GGTACC G^G[CT][GA]CC
BbvCI 4171 CCTCAGC CCTCAGC(-5/-2)
BcgI 4114 GCAGTGCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2150 CTTAAG C^TTAAG
BglI 1294 GCCTTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2853 TACGTG [CT]AC^GT[GA]
BsaBI 1966 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 492 GGTCTC GGTCTC(1/5)
BsgI 3200 CTGCAC GTGCAG(16/14)
BsiHKAI 765 GAGCTC G[AT]GC[AT]^C
BsoBI 138 CTCGAG C^[CT]CG[GA]G
BspDI 215 ATCGAT AT^CGAT
BsrFI 379 GCCGGT [GA]^CCGG[CT]
BssSI 4178 CTCGTG CACGAG(-5/-1)
Bst1107I 3784 GTATAC GTA^TAC
BstAPI 3867 GCAACGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1788 TTCGAA TT^CGAA
BstEII 859 GGTGACC G^GT[ACGT]ACC
BstPI 859 GGTGACC G^GT[ACGT]ACC
BstXI 1860 CCAAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3784 GTATAC GTA^TAC
CfoI 1012 GCGC GCG^C
Cfr10I 379 GCCGGT [GA]^CCGG[CT]
ClaI 215 ATCGAT AT^CGAT
DraII 1394 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 91 GACCAGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1799 CCATGG C^C[GA][CT]GG
EaeI 153 TGGCCA [CT]^GGCC[GA]
Eco47III 1011 AGCGCT AGC^GCT
EcoO109I 1394 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 859 GGTGACC G^GT[ACGT]ACC
HaeII 1011 AGCGCT [GA]GCGC^[CT]
HapII 380 CCGG C^CGG
HhaI 1012 GCGC GCG^C
HinP1I 1012 GCGC G^CGC
HincII 4276 GTTAAC GT[CT]^[GA]AC
HindII 4276 GTTAAC GT[CT]^[GA]AC
HindIII 2995 AAGCTT A^AGCTT
HpaI 4276 GTTAAC GTT^AAC
HpaII 380 CCGG C^CGG
KpnI 844 GGTACC GGTAC^C
MamI 1966 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 153 TGGCCA TGG^CCA
MscI 153 TGGCCA TGG^CCA
MspI 380 CCGG C^CGG
NcoI 1799 CCATGG C^CATGG
NspI 4203 GCATGC [GA]CATG^[CT]
NspV 1788 TTCGAA TT^CGAA
PaeR7I 138 CTCGAG C^TCGAG
PflFI 4078 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2053 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3869 AACGTT AA^CGTT
PvuII 1544 CAGCTG CAG^CTG
SacI 765 GAGCTC GAGCT^C
SexAI 2464 ACCAGGT A^CC[AT]GGT
SfuI 1788 TTCGAA TT^CGAA
SphI 4203 GCATGC GCATG^C
StuI 1113 AGGCCT AGG^CCT
Tth111I 4078 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 138 CTCGAG C^TCGAG
XmnI 1239 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 452 CGCG CG^CG
4124 CGCG
AcyI 538 GACGTC G[GA]^CG[CT]C
696 GACGCC
AlwNI 1264 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
2684 CAGAAGCTG
AocI 688 CCTGAGG CC^T[ACGT]AGG
1753 CCTCAGG
BanII 765 GAGCTC G[GA]GC[CT]^C
4151 GGGCTC
BclI 3354 TGATCA T^GATCA
3923 TGATCA
BmyI 765 GAGCTC G[AGT]GC[ACT]^C
4151 GGGCTC
BsaHI 538 GACGTC G[GA]^CG[CT]C
696 GACGCC
BseRI 2406 CTCCTC GAGGAG(10/8)
691 GAGGAG
BsmBI 487 GAGACG CGTCTC(1/5)
694 GAGACG
Bsp1286I 765 GAGCTC G[AGT]GC[ACT]^C
4151 GGGCTC
BsrDI 3387 CATTGC GCAATG(2/0)
1465 GCAATG
BstF5I 1359 CATCC GGATG(2/0)
240 GGATG
BstUI 452 CGCG CG^CG
4124 CGCG
BstYI 414 AGATCC [GA]^GATC[CT]
1124 GGATCC
Bsu36I 688 CCTGAGG CC^T[ACGT]AGG
1753 CCTCAGG
Eco81I 688 CCTGAGG CC^T[ACGT]AGG
1753 CCTCAGG
EcoT22I 1499 ATGCAT ATGCA^T
2882 ATGCAT
FbaI 3354 TGATCA T^GATCA
3923 TGATCA
FokI 1359 CATCC GGATG(9/13)
240 GGATG
HgaI 450 GACGC GACGC(5/10)
696 GACGC
Hin1I 538 GACGTC G[GA]^CG[CT]C
696 GACGCC
MflI 414 AGATCC [GA]^GATC[CT]
1124 GGATCC
MslI 1953 CACTTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3358 CACTTAAATG
MspA1I 81 CAGCGG C[AC]G^C[GT]G
1544 CAGCTG
MvnI 452 CGCG CG^CG
4124 CGCG
NdeI 2277 CATATG CA^TATG
3848 CATATG
NsiI 1499 ATGCAT ATGCA^T
2882 ATGCAT
PflMI 29 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
51 CCAGGGAGTGG
PstI 2036 CTGCAG CTGCA^G
4133 CTGCAG
SapI 786 GAAGAGC GCTCTTC(1/4)
1242 GCTCTTC
ScaI 3056 AGTACT AGT^ACT
3231 AGTACT
Van91I 29 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
51 CCAGGGAGTGG
XcmI 185 CCATCAAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1984 CCAAATGCTGAGTGG
XhoII 414 AGATCC [GA]^GATC[CT]
1124 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AvaII
BbsI BbvI BfaI BpmI BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
BsmI BspHI BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I Fnu4HI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI PshBI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I