##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AflIII AgeI AhdI
AocI AscI Asp718I AspEI AspI
AvaI AviII BamHI BbrPI BcgI
BlpI Bpu1102I BsiWI BsoBI BspLU11I
BspMI BssHII BssSI BstEII BstPI
Bsu36I CelII CpoI Eam1105I Eco81I
EcoO65I FseI FspI KpnI KspI
MluI NheI NotI NruI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacII SalI
SbfI SexAI SfiI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 607 TCCGGA T^CCGGA
AclI 5349 AACGTT AA^CGTT
Aor51HI 133 AGCGCT AGC^GCT
ApaI 240 GGGCCC GGGCC^C
BalI 2390 TGGCCA TGG^CCA
BglII 1308 AGATCT A^GATCT
BsaI 2140 GGTCTC GGTCTC(1/5)
BsaWI 607 TCCGGA [AT]^CCGG[AT]
BseAI 607 TCCGGA T^CCGGA
BseRI 3643 GAGGAG GAGGAG(10/8)
BsmI 112 GAATGC GAATGC(1/-1)
BspDI 2059 ATCGAT AT^CGAT
BspEI 607 TCCGGA T^CCGGA
BspHI 767 TCATGA T^CATGA
BsrBI 438 GAGCGG CCGCTC(-3/-3)
BsrFI 262 GCCGGC [GA]^CCGG[CT]
Bst1107I 4976 GTATAC GTA^TAC
BstBI 1960 TTCGAA TT^CGAA
BstXI 1077 CCAGATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4976 GTATAC GTA^TAC
Cfr10I 262 GCCGGC [GA]^CCGG[CT]
ClaI 2059 ATCGAT AT^CGAT
DraIII 3714 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 133 AGCGCT AGC^GCT
EcoNI 645 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2032 ATGCAT ATGCA^T
MfeI 4370 CAATTG C^AATTG
MluNI 2390 TGGCCA TGG^CCA
MroI 607 TCCGGA T^CCGGA
MscI 2390 TGGCCA TGG^CCA
MunI 4370 CAATTG C^AATTG
NaeI 262 GCCGGC GCC^GGC
NgoMIV 262 GCCGGC G^CCGGC
NsiI 2032 ATGCAT ATGCA^T
NspV 1960 TTCGAA TT^CGAA
PpuMI 2996 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 5349 AACGTT AA^CGTT
RcaI 767 TCATGA T^CATGA
SacI 1240 GAGCTC GAGCT^C
SapI 2922 GCTCTTC GCTCTTC(1/4)
ScaI 5025 AGTACT AGT^ACT
SfuI 1960 TTCGAA TT^CGAA
StuI 5012 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 3357 GTAGAC GT^[AC][GT]AC
4976 GTATAC
AccII 123 CGCG CG^CG
470 CGCG
AflII 480 CTTAAG C^TTAAG
4930 CTTAAG
Alw44I 1966 GTGCAC G^TGCAC
4909 GTGCAC
ApaLI 1966 GTGCAC G^TGCAC
4909 GTGCAC
AvrII 1782 CCTAGG C^CTAGG
4566 CCTAGG
BbeI 327 GGCGCC GGCGC^C
2176 GGCGCC
BciVI 1808 GGATAC GTATCC(6/5)
1592 GTATCC
BfrI 480 CTTAAG C^TTAAG
4930 CTTAAG
BglI 257 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5014 GCCTTGTTGGC
BlnI 1782 CCTAGG C^CTAGG
4566 CCTAGG
BsaAI 540 CACGTA [CT]AC^GT[GA]
3460 TACGTG
BsiEI 37 CGGCCG CG[GA][CT]^CG
250 CGGCCG
BsmBI 92 CGTCTC CGTCTC(1/5)
450 CGTCTC
Bsp1407I 365 TGTACA T^GTACA
3327 TGTACA
BsrGI 365 TGTACA T^GTACA
3327 TGTACA
BstAPI 1728 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1752 GCAGTGTCTGC
BstUI 123 CGCG CG^CG
470 CGCG
DrdI 393 GACTCCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2666 GACTGATTTGTC
EagI 37 CGGCCG C^GGCCG
250 CGGCCG
EarI 2923 CTCTTC CTCTTC(1/4)
3021 GAAGAG
EclXI 37 CGGCCG C^GGCCG
250 CGGCCG
Eco52I 37 CGGCCG C^GGCCG
250 CGGCCG
HindIII 612 AAGCTT A^AGCTT
5405 AAGCTT
HpaI 2514 GTTAAC GTT^AAC
5352 GTTAAC
KasI 327 GGCGCC G^GCGCC
2176 GGCGCC
Ksp632I 2923 CTCTTC CTCTTC(1/4)
3021 GAAGAG
MvnI 123 CGCG CG^CG
470 CGCG
NarI 327 GGCGCC GG^CGCC
2176 GGCGCC
PacI 2790 TTAATTAA TTAAT^TAA
5393 TTAATTAA
PflMI 2406 CCAGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3183 CCACTTACTGG
SfoI 327 GGCGCC GGC^GCC
2176 GGCGCC
SpeI 2738 ACTAGT A^CTAGT
3687 ACTAGT
SphI 2087 GCATGC GCATG^C
4527 GCATGC
SspBI 365 TGTACA T^GTACA
3327 TGTACA
Van91I 2406 CCAGCGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3183 CCACTTACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BanI BanII
BbsI BbvCI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaBI
BsaHI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EcoO109I
EcoRI EcoRII EcoRV EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI