##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AviII BlpI Bpu1102I BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrBI BsrGI BssHII Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DraI DrdI Eam1105I EcoRI EcoT22I
FseI FspI HpaI KpnI MfeI
MroI MunI NheI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 2124 GTAGAC GT^[AC][GT]AC
AflII 272 CTTAAG C^TTAAG
Alw44I 1788 GTGCAC G^TGCAC
ApaLI 1788 GTGCAC G^TGCAC
AvaI 821 CCCGGG C^[CT]CG[GA]G
AvrII 131 CCTAGG C^CTAGG
BamHI 811 GGATCC G^GATCC
BbeI 3265 GGCGCC GGCGC^C
BbrPI 2937 CACGTG CAC^GTG
BbvCI 3240 GCTGAGG CCTCAGC(-5/-2)
BcgI 1294 CGAGCACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1627 GTATCC GTATCC(6/5)
BclI 334 TGATCA T^GATCA
BfrI 272 CTTAAG C^TTAAG
BlnI 131 CCTAGG C^CTAGG
BsaAI 2937 CACGTG [CT]AC^GT[GA]
BsiEI 18 CGGCCG CG[GA][CT]^CG
BsoBI 821 CCCGGG C^[CT]CG[GA]G
BspLU11I 563 ACATGT A^CATGT
BssSI 3213 CACGAG CACGAG(-5/-1)
BstEII 639 GGTCACC G^GT[ACGT]ACC
BstPI 639 GGTCACC G^GT[ACGT]ACC
DraIII 366 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 18 CGGCCG C^GGCCG
EclXI 18 CGGCCG C^GGCCG
Eco52I 18 CGGCCG C^GGCCG
EcoO65I 639 GGTCACC G^GT[ACGT]ACC
EcoRV 1326 GATATC GAT^ATC
FbaI 334 TGATCA T^GATCA
HincII 573 GTTGAC GT[CT]^[GA]AC
HindII 573 GTTGAC GT[CT]^[GA]AC
KasI 3265 GGCGCC G^GCGCC
KspI 785 CCGCGG CCGC^GG
NaeI 111 GCCGGC GCC^GGC
NarI 3265 GGCGCC GG^CGCC
NdeI 1377 CATATG CA^TATG
NgoMIV 111 GCCGGC G^CCGGC
NotI 17 GCGGCCGC GC^GGCCGC
PmaCI 2937 CACGTG CAC^GTG
PmlI 2937 CACGTG CAC^GTG
PshAI 2127 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 785 CCGCGG CCGC^GG
SfiI 1763 GGCCTCCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3265 GGCGCC GGC^GCC
SgrAI 110 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 821 CCCGGG CCC^GGG
XbaI 2156 TCTAGA T^CTAGA
XmaCI 821 CCCGGG C^CCGGG
XmaI 821 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 609 GACGTC GACGT^C
1512 GACGTC
AcsI 2085 GAATTT [GA]^AATT[CT]
3528 AAATTC
Aor51HI 550 AGCGCT AGC^GCT
3220 AGCGCT
ApoI 2085 GAATTT [GA]^AATT[CT]
3528 AAATTC
Asp700I 2447 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2799 GAATATATTC
AspI 374 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2979 GACTCAGTC
BglII 280 AGATCT A^GATCT
2500 AGATCT
BsmBI 36 CGTCTC CGTCTC(1/5)
2594 CGTCTC
BsrFI 111 GCCGGC [GA]^CCGG[CT]
1162 ACCGGC
Cfr10I 111 GCCGGC [GA]^CCGG[CT]
1162 ACCGGC
Eco47III 550 AGCGCT AGC^GCT
3220 AGCGCT
HindIII 484 AAGCTT A^AGCTT
2508 AAGCTT
MluI 748 ACGCGT A^CGCGT
2167 ACGCGT
NcoI 124 CCATGG C^CATGG
1345 CCATGG
NspI 563 ACATGT [GA]CATG^[CT]
859 ACATGC
PflFI 374 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2979 GACTCAGTC
PflMI 2231 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2315 CCATATCCTGG
SacI 2001 GAGCTC GAGCT^C
2325 GAGCTC
SapI 2447 GAAGAGC GCTCTTC(1/4)
2055 GCTCTTC
StuI 7 AGGCCT AGG^CCT
268 AGGCCT
Tth111I 374 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2979 GACTCAGTC
Van91I 2231 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2315 CCATATCCTGG
XcmI 760 CCATGCCCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2752 CCAGGAGACTGCTGG
XmnI 2447 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2799 GAATATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaBI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII