################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## Acc65I AccI AclI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII BalI BbeI BbrPI BcgI BciVI BfrI BglI BsaAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoRI FseI FspI HaeII KasI KpnI KspI MfeI MluI MluNI MscI MunI NaeI NarI NcoI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 2083 GACGTC GACGT^C AccII 219 CGCG CG^CG AccIII 1619 TCCGGA T^CCGGA AcyI 2083 GACGTC G[GA]^CG[CT]C Alw44I 542 GTGCAC G^TGCAC AlwNI 2301 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2065 CCTAAGG CC^T[ACGT]AGG ApaI 728 GGGCCC GGGCC^C ApaLI 542 GTGCAC G^TGCAC AspI 2797 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1607 CCTAGG C^CTAGG BanI 2678 GGCACC G^G[CT][GA]CC BclI 281 TGATCA T^GATCA BglII 2344 AGATCT A^GATCT BlnI 1607 CCTAGG C^CTAGG BpmI 2774 CTCCAG CTGGAG(16/14) BsaBI 3640 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2083 GACGTC G[GA]^CG[CT]C BsaWI 1619 TCCGGA [AT]^CCGG[AT] BseAI 1619 TCCGGA T^CCGGA BspEI 1619 TCCGGA T^CCGGA BsrFI 488 ACCGGC [GA]^CCGG[CT] BstEII 450 GGTGACC G^GT[ACGT]ACC BstPI 450 GGTGACC G^GT[ACGT]ACC BstUI 219 CGCG CG^CG Bsu36I 2065 CCTAAGG CC^T[ACGT]AGG CfoI 220 GCGC GCG^C Cfr10I 488 ACCGGC [GA]^CCGG[CT] DraI 1686 TTTAAA TTT^AAA DrdI 1229 GACCCATAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2065 CCTAAGG CC^T[ACGT]AGG EcoNI 324 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 450 GGTGACC G^GT[ACGT]ACC EcoRV 2860 GATATC GAT^ATC EcoT22I 1567 ATGCAT ATGCA^T FauI 3441 GCGGG CCCGC(4/6) FbaI 281 TGATCA T^GATCA HgaI 301 GACGC GACGC(5/10) HhaI 220 GCGC GCG^C Hin1I 2083 GACGTC G[GA]^CG[CT]C HinP1I 220 GCGC G^CGC MaeII 2084 ACGT A^CGT MamI 3640 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1619 TCCGGA T^CCGGA MvnI 219 CGCG CG^CG NheI 1924 GCTAGC G^CTAGC NsiI 1567 ATGCAT ATGCA^T PflFI 2797 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 243 AGGACCT [GA]G^G[AT]CC[CT] PshAI 708 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 2816 GAAGAGC GCTCTTC(1/4) ScaI 3765 AGTACT AGT^ACT SexAI 264 ACCAGGT A^CC[AT]GGT SpeI 85 ACTAGT A^CTAGT SspI 2332 AATATT AAT^ATT Tth111I 2797 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BamHI 186 GGATCC G^GATCC 1895 GGATCC BbvCI 1951 CCTCAGC CCTCAGC(-5/-2) 2706 GCTGAGG BlpI 2306 GCTGAGC GC^T[ACGT]AGC 2324 GCTTAGC Bpu1102I 2306 GCTGAGC GC^T[ACGT]AGC 2324 GCTTAGC BsaI 902 GGTCTC GGTCTC(1/5) 1237 GGTCTC BsmFI 299 GGGAC GGGAC(10/14) 2015 GTCCC BspMI 78 GCAGGT ACCTGC(4/8) 2949 GCAGGT CelII 2306 GCTGAGC GC^T[ACGT]AGC 2324 GCTTAGC DraII 243 AGGACCT [GA]G^G[ACGT]CC[CT] 727 AGGGCCC EcoO109I 243 AGGACCT [GA]G^G[ACGT]CC[CT] 727 AGGGCCC HindIII 3534 AAGCTT A^AGCTT 3781 AAGCTT HpaI 3342 GTTAAC GTT^AAC 3434 GTTAAC TaqI 1555 TCGA T^CGA 2279 TCGA TthHB8I 1555 TCGA T^CGA 2279 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmI Bsp1286I BspHI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII