##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII BalI BbeI BbrPI BcgI
BciVI BfrI BglI BsaAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI FseI
FspI HaeII KasI KpnI KspI
MfeI MluI MluNI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI SwaI XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 2083 GACGTC GACGT^C
AccII 219 CGCG CG^CG
AccIII 1619 TCCGGA T^CCGGA
AcyI 2083 GACGTC G[GA]^CG[CT]C
Alw44I 542 GTGCAC G^TGCAC
AlwNI 2301 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2065 CCTAAGG CC^T[ACGT]AGG
ApaI 728 GGGCCC GGGCC^C
ApaLI 542 GTGCAC G^TGCAC
AspI 2797 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1607 CCTAGG C^CTAGG
BanI 2678 GGCACC G^G[CT][GA]CC
BclI 281 TGATCA T^GATCA
BglII 2344 AGATCT A^GATCT
BlnI 1607 CCTAGG C^CTAGG
BpmI 2774 CTCCAG CTGGAG(16/14)
BsaBI 3640 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2083 GACGTC G[GA]^CG[CT]C
BsaWI 1619 TCCGGA [AT]^CCGG[AT]
BseAI 1619 TCCGGA T^CCGGA
BspEI 1619 TCCGGA T^CCGGA
BsrFI 488 ACCGGC [GA]^CCGG[CT]
BstEII 450 GGTGACC G^GT[ACGT]ACC
BstPI 450 GGTGACC G^GT[ACGT]ACC
BstUI 219 CGCG CG^CG
Bsu36I 2065 CCTAAGG CC^T[ACGT]AGG
CfoI 220 GCGC GCG^C
Cfr10I 488 ACCGGC [GA]^CCGG[CT]
DraI 1686 TTTAAA TTT^AAA
DrdI 1229 GACCCATAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2065 CCTAAGG CC^T[ACGT]AGG
EcoNI 324 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 450 GGTGACC G^GT[ACGT]ACC
EcoRV 2860 GATATC GAT^ATC
EcoT22I 1567 ATGCAT ATGCA^T
FauI 3441 GCGGG CCCGC(4/6)
FbaI 281 TGATCA T^GATCA
HgaI 301 GACGC GACGC(5/10)
HhaI 220 GCGC GCG^C
Hin1I 2083 GACGTC G[GA]^CG[CT]C
HinP1I 220 GCGC G^CGC
MaeII 2084 ACGT A^CGT
MamI 3640 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1619 TCCGGA T^CCGGA
MvnI 219 CGCG CG^CG
NheI 1924 GCTAGC G^CTAGC
NsiI 1567 ATGCAT ATGCA^T
PflFI 2797 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 243 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 708 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2816 GAAGAGC GCTCTTC(1/4)
ScaI 3765 AGTACT AGT^ACT
SexAI 264 ACCAGGT A^CC[AT]GGT
SpeI 85 ACTAGT A^CTAGT
SspI 2332 AATATT AAT^ATT
Tth111I 2797 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BamHI 186 GGATCC G^GATCC
1895 GGATCC
BbvCI 1951 CCTCAGC CCTCAGC(-5/-2)
2706 GCTGAGG
BlpI 2306 GCTGAGC GC^T[ACGT]AGC
2324 GCTTAGC
Bpu1102I 2306 GCTGAGC GC^T[ACGT]AGC
2324 GCTTAGC
BsaI 902 GGTCTC GGTCTC(1/5)
1237 GGTCTC
BsmFI 299 GGGAC GGGAC(10/14)
2015 GTCCC
BspMI 78 GCAGGT ACCTGC(4/8)
2949 GCAGGT
CelII 2306 GCTGAGC GC^T[ACGT]AGC
2324 GCTTAGC
DraII 243 AGGACCT [GA]G^G[ACGT]CC[CT]
727 AGGGCCC
EcoO109I 243 AGGACCT [GA]G^G[ACGT]CC[CT]
727 AGGGCCC
HindIII 3534 AAGCTT A^AGCTT
3781 AAGCTT
HpaI 3342 GTTAAC GTT^AAC
3434 GTTAAC
TaqI 1555 TCGA T^CGA
2279 TCGA
TthHB8I 1555 TCGA T^CGA
2279 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmI Bsp1286I BspHI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII